LOCUS BBa_M1007 4543 bp DNA linear 17-May-2024 DEFINITION Complete Phasin Secretion Construct ACCESSION BBa_M1007 VERSION BBa_M1007.1 FEATURES Location/Qualifiers promoter 1..35 /label=BBa_J23100 RBS 44..57 /label=BBa_B0035 misc_feature 1..57 /label=BBa_M1005 misc_feature 64..66 /label=Start CDS 64..642 /label=Phasin CDS 64..642 /label=BBa_K208001 misc_feature 651..836 /label=HlyA misc_feature 651..653 /label=Start misc_feature 651..836 /label=BBa_K208006 promoter 845..879 /label=BBa_J23100 RBS 888..901 /label=BBa_B0035 misc_feature 845..901 /label=BBa_M1005 misc_feature 908..910 /label=Start Codon misc_feature 3029..3031 /label=Stop Codon CDS 908..3028 /label=HlyB CDS 908..3031 /label=BBa_M1003 RBS 3040..3053 /label=BBa_B0035 misc_feature 3060..3062 /label=Start Codon CDS 3060..4493 /label=HlyD misc_feature 4494..4496 /label=Stop Codon CDS 3060..4496 /label=BBa_M1004 misc_feature 4505..4543 /label=B1006 stem_loop 4514..4535 /label=modified thr terminator polyA_site 4536..4543 /label=PolyA polyA_site 4505..4513 /label=PolyA terminator 4505..4543 /label=BBa_B1006 misc_feature 845..4543 /label=BBa_M1006 ORIGIN 1 ttgacggcta gctcagtcct aggtacagtg ctagctacta gagattaaag aggagaatac 61 tagatgatcc tcaccccgga acaagttgca gcagcgcaaa aggccaacct cgaaacgctg 121 ttcggcctga ccaccaaggc gtttgaaggc gtcgaaaagc tcgtcgagct gaaccttcag 181 gtcgtcaaga cttcgttcgc agaaggcgtt gacaacgcca agaaggcgct gtcggccaag 241 gacgcacagg aactgctggc catccaggcc gcagccgtgc agccggttgc cgaaaagacc 301 ctggcctaca cccgccacct gtatgaaatc gcttcggaaa cccagagcga gttcaccaag 361 gtagccgagg ctcaactggc cgaaggctcg aagaacgtgc aagcgctggt cgagaacctc 421 gccaagaacg ccccggccgg ttcggaatcg accgtggcca tcgtgaagtc ggcgatctcc 481 gctgccaaca acgcctacga gtcggtgcag aaggcgacca agcaagcggt cgaaatcgct 541 gaaaccaact tccaggctgc ggctacggct gccaccaagg ctgcccagca agccagcgcc 601 acggcccgta cggccacggc aaagaagacg acggctgcct gatactagag ttagcctatg 661 gaagtcaggg tgatcttaat ccattaatta atgaaatcag caaaatcatt tcagcagcag 721 gtagcttcga tgttaaagag gaaagaaccg cagcttcttt attgcagttg tccggtaatg 781 ccagtgattt ttcatatgga cggaactcaa taaccctgac cacatcagca taataatact 841 agagttgacg gctagctcag tcctaggtac agtgctagct actagagatt aaagaggaga 901 atactagatg gattcctgtc acaagattga ttacggcctg tatgcactgg aaatcctggc 961 acaatatcat aacgtctccg tcaacccgga ggaaattaaa catcgcttcg atactgacgg 1021 cactggcctg ggtctgacca gctggctgct ggcagcgaag tccctggagc tgaaggtaaa 1081 gcaagtgaaa aagacgattg atcgtctgaa cttcatcagc ctgccggcac tggtttggcg 1141 tgaagatggc cgtcacttca tcctgacgaa agtgtccaaa gaagcgaacc gctacctgat 1201 ctttgatctg gagcagcgca acccgcgcgt actggaacag agcgagttcg aggctctgta 1261 tcagggccac atcattctga ttgcatctcg ttcttctgtt gcaggtaaac tggcgaaatt 1321 cgacttcacc tggttcattc cggctattat caagtaccgt cgtattttca tcgaaaccct 1381 ggttgtatct gttttcctgc aactgttcgc tctgattact ccgctgttct ttcaggttgt 1441 catggacaaa gttctggttc accgtggctt ttccaccctg aacgtcatta ctgttgcgct 1501 gagcgtggtt gttgtgttcg aaatcattct gtccggtctg cgtacctaca tcttcgcgca 1561 ctctacctcc cgcatcgacg ttgaactggg tgcgaagctg ttccgtcacc tgctggcact 1621 gcctatcagc tacttcgaat ctcgtcgtgt gggcgatact gtggcccgtg ttcgtgaact 1681 ggatcagatc cgcaactttc tgaccggcca ggccctgacc agcgttctgg atctgctgtt 1741 cagctttatc ttcttcgcag taatgtggta ctacagcccg aagctgactc tggtgattct 1801 gttctctctg ccgtgttacg cagcttggtc cgtcttcatc tctccaatcc tgcgtcgccg 1861 tctggacgac aaatttagcc gtaatgcgga taatcagtcc ttcctggtcg aatctgtgac 1921 tgctatcaac actatcaaag ccatggcggt ttctccacag atgactaaca tctgggataa 1981 gcagctggca ggttacgttg ctgcgggctt caaagtgacc gttctggcaa ccattggtca 2041 acagggcatt cagctgattc agaaaaccgt gatgatcatt aacctgtggc tgggtgcgca 2101 cctggtcatc agcggtgacc tgtccattgg ccagctgatc gctttcaaca tgctggcggg 2161 tcaaattgtt gccccagtaa tccgtctggc ccagatctgg caggacttcc agcaggtcgg 2221 tatttctgtg acccgcctgg gtgacgtcct gaactctccg accgaaagct accacggtaa 2281 actggctctg ccggagatca acggcgatat tactttccgt aacatccgtt ttcgttacaa 2341 accggattcc ccggtaatcc tggataacat caatctgtct atcaaacagg gcgaagtcat 2401 cggcatcgtg ggccgtagcg gcagcggcaa gtccactctg actaaactga ttcagcgctt 2461 ttacattcca gaaaacggtc aggttctgat tgacggtcac gacctggctc tggcggaccc 2521 gaactggctg cgtcgtcagg tgggcgtagt gctgcaagat aacgtgctgc tgaaccgtag 2581 catcatcgat aacatcagcc tggctaaccc gggcatgtcc gtggaaaaag ttatctatgc 2641 tgcgaaactg gcaggcgcgc acgatttcat ctctgaactg cgtgaaggct acaacaccat 2701 cgtaggcgaa cagggcgctg gcctgtccgg cggtcaacgt cagcgtatcg ctattgcacg 2761 tgcactggtg aacaacccga aaatcctgat cttcgacgag gccacttctg ctctggatta 2821 cgaatctgaa catattatca tgcgtaatat gcacaagatc tgcaaaggtc gcaccgtaat 2881 catcatcgcc caccgtctga gcaccgtaaa gaacgccgac cgcatcatcg tgatggaaaa 2941 aggtaagatc gttgaacagg gtaaacataa agagctgctg tccgaaccgg aatctctgta 3001 ttcttatctg taccagctgc aatctgatta atactagaga ttaaagagga gaatactaga 3061 tgaaaacttg gctgatgggc ttctctgagt tcctgctgcg ttataagctg gtatggagcg 3121 aaacttggaa aatccgtaag caactggata ccccggtacg tgaaaaagac gaaaacgaat 3181 ttctgccagc acatctggaa ctgattgaga cgccggtttc tcgccgtcca cgtctggttg 3241 cttacttcat tatgggcttc ctggttattg ctgtaatcct gtctgttctg ggtcaggtgg 3301 aaatcgttgc gaccgcgaat ggtaaactga ccctgtccgg tcgttctaaa gaaatcaaac 3361 cgatcgaaaa ctccatcgtt aaagagatta tcgttaaaga aggtgaatcc gtacgcaaag 3421 gcgatgttct gctgaaactg accgcactgg gcgcagaagc ggacactctg aaaactcagt 3481 cctctctgct gcaaacccgt ctggaacaga cccgttacca aatcctgagc cgttctattg 3541 aactgaacaa actgccggaa ctgaaactgc cggatgaacc atacttccag aacgtaagcg 3601 aggaggaagt tctgcgcctg acttccctga tcaaagagca gttctctact tggcagaacc 3661 agaaatacca aaaggaactg aacctggaca aaaagcgtgc ggagcgcctg actatcctgg 3721 cccgcatcaa ccgttacgag aacctgtctc gtgttgaaaa atctcgcctg gacgacttcc 3781 gtagcctgct gcacaaacag gccatcgcaa aacacgctgt gctggaacag gaaaataaat 3841 acgtggaagc tgccaacgaa ctgcgtgtat ataaaagcca gctggagcag atcgagagcg 3901 aaatcctgtc tgcgaaggag gagtaccagc tggtgacgca gctgttcaaa aatgaaatcc 3961 tggacaaact gcgtcaaacc acggactcta tcgaactgct gaccctggaa ctggagaaaa 4021 acgaggagcg ccagcaggcc agcgtcatcc gcgctccggt atctggtaaa gttcagcaac 4081 tgaaagttca caccgaaggt ggcgttgtta ccaccgcaga aaccctgatg gttattgtgc 4141 cggaagatga caccctggaa gttaccgctc tggtgcagaa caaagatatc ggtttcatca 4201 acgtcggtca gaacgcgatc atcaaagtag aggctttccc gtacactcgt tacggctacc 4261 tggtgggcaa ggtcaaaaac atcaacctgg acgctatcga agatcagaaa ctgggcctgg 4321 tgttcaacgt gatcgttagc gtagaagaga acgatctgtc taccggtaac aaacacattc 4381 cactgtcctc cggcatggcg gttactgctg aaatcaaaac cggtatgcgt agcgtgatct 4441 cctatctgct gtctccgctg gaagaaagcg tcaccgaatc tctgcacgaa cgctaatact 4501 agagaaaaaa aaaccccgcc cctgacaggg cggggttttt ttt //