LOCUS BBa_M1115 5420 bp DNA linear 18-May-2024 DEFINITION Final lignin degradation construct ACCESSION BBa_M1115 VERSION BBa_M1115.1 FEATURES Location/Qualifiers promoter 1..35 /label=BBa_J23110 RBS 44..58 /label=RBS-1\Strong RBS 51..55 /label=RBS misc_feature 44..58 /label=BBa_B0030 RBS 44..58 /label=BBa_B0030 misc_feature 65..67 /label=start codon CDS 65..1640 /label=BBa_M1130 RBS 1649..1663 /label=RBS-1\Strong RBS 1656..1660 /label=RBS misc_feature 1649..1663 /label=BBa_B0030 RBS 1649..1663 /label=BBa_B0030 misc_feature 1672..1674 /label=start codon misc_feature 3046..3048 /label=stop codon misc_feature 1675..3045 /label=coding region CDS 1672..3048 /label=BBa_M1112 RBS 3057..3071 /label=RBS-1\Strong RBS 3064..3068 /label=RBS misc_feature 3057..3071 /label=BBa_B0030 RBS 3057..3071 /label=BBa_B0030 misc_feature 3078..5373 /label=coding region misc_feature 3078..3080 /label=start codon misc_feature 5371..5373 /label=stop codon CDS 3078..5373 /label=BBa_M1113 misc_feature 5382..5420 /label=B1006 stem_loop 5391..5412 /label=modified thr terminator polyA_site 5413..5420 /label=PolyA polyA_site 5382..5390 /label=PolyA terminator 5382..5420 /label=BBa_B1006 ORIGIN 1 tttacggcta gctcagtcct aggtacaatg ctagctacta gagattaaag aggagaaata 61 ctagatggct ttcaactttg ctgcaatcct cgcctttgtc tcactcgccg ccgtgacgag 121 tgctgccccg agcgggacca cctgttccaa tggtgtagtc gttcctgacg cagtgtgctg 181 cgacttcgtc ccagtaaggc attctgctcc tgtcttttta tgcagcacac tgaatcgtcg 241 tacgaaatag cttgcatctg cccttcagag cgaggtcctc atgggcgact gtggtgaaga 301 cggtaggcca agttcctgtc atcctatccc tgaaccgctg tctcacttgc tctccagcgc 361 acgagctcgt ccgtcttatt ttccgtaagt ctggctcgtc aatcgtctcc ggtattgtat 421 tgacactatg ttagacgatg ccattgccat ctcccaaagc atgggtccct cagcgtaagt 481 gaaaactctc gttaaaattg gtttgtgctg atttgactcg gcctacagtg gtggcggagc 541 cgacggctcg atgctcatct tccctacggt cgaaccggcg ttcttcccga accttggtat 601 cgccgacagc gtcaacaact tgatcccctt cctctcacaa ttccccacga tttctgctgg 661 tgatcttgtt cagttcgcag gcgccgtcgc gatcagcaac tgccccgtaa gcagctacag 721 cgctcggtct tctactgaac cgatgctgac acgaattgtg catacagggc gcccctcaac 781 tggagttcct tgccggacgt tccaacgcga ccgctcctgc catcgacggc ctcatccccg 841 agccccagga cgatgtcacc aagattcttg cgcgctttgc ggatgctggc aacttcaccc 901 ctgacgaagt cgttgctctc ctggcttcgc acagtatcgc ccgtgctgat cacgtcgacc 961 cgacgcttga cgccgcgccc ttcgactccg tgagtggtgc ctattgcccg cccctctttc 1021 accggctgac ttcatctacc atcccacaga cgccgttcga cttcgacacc caggtcttcc 1081 ttgaggtgct gctcaagggc accggcttcc ccggcctgga caacaacacc ggcgaggtct 1141 cctccccgct ccccgtcacc gatggcaccg atgtcggcga gctgcgcctc cagtccgact 1201 tcgtgcttgc ccgcgacgag cgcaccgcct gcgcctggca gagcttcgtc aacgaacagc 1261 aggcgatggc tgatgcgttc aaggaggcag tcaagaagct cgccgtgctc ggccacagcc 1321 gcagcgacct cgtcgactgc tccgcggtcg tccctgtccc caagcccgcg actggcacgc 1381 ccgccacgtt ccccgcttcg acgggcccgc aggacctcga gctcacgtgc acgaccgtgc 1441 cgttccccac gctgtctact gcccgtaagt ctcgctgttt tctctcatct atgatctgca 1501 ccgttgctaa tcgcccactc ttcatcacag ccggcgccca gcagactctg atcccgcact 1561 gctccgacgg caccatgact tgcaactccg tgcagttcga cggccccgcc accaacttcg 1621 gtggtgcaga cgactcgtag tactagagat taaagaggag aaatactaga ggtgtcgcag 1681 gcgccccgaa ctccccggac tcgctacgcc aggtgcggcg acctggacat cgcctaccag 1741 gtgatcgggg atcatccgat cgacctgctc gtcataccgg gggcctcgat tccggtcgac 1801 accatcgatt ccgaaccgtc gatgtaccgc tttcaccgcc gtctggcgtc gttttcccgc 1861 ctgatccggt tcgaccatcg cggcgtcggc ctgtcatcgc gggtttcgtc gccggatgcg 1921 ctcggcccga ggttctgggc cgaggatgcg attgcggtga tggacgcggc cggatgccag 1981 gaagccacga ttcttgcctc cgggttcacc gcgacgaccg ccctggtgct ggccgccgac 2041 tatcccgagc gggtgcgcag cctggtgctc atcaacgcct ccgcgcggac actgcatgcg 2101 cccgactacg aactgggcat caggtccaac accgccgaac cattcctcac cctcggaacc 2161 gatcccgacg cggtcgagca ggggttcgac gtgctgcgca tcatggcgcc cagcgtggcc 2221 catgacgacg cgttccgcga ctggtgggat ctggccggaa accgggcggc ctcgcccagc 2281 acggcgcgcg ccttcatcaa cgcggttcaa gcctcggatg cgcgcgactc gctgccccac 2341 atcaccgcgc cgacgctgat cctgcatcgg gtgggcacca agttcgttcc ggtcgagcac 2401 ggccgctatc tggccgagca cattgccggg tcgcgcttgg tcgagcttcc cggttccgat 2461 tccttgtatt gggtcggcga caccgccgcg ttgctcgacg aggtcgagga gttcatcacc 2521 ggcgtccggg gcggcttcgt caccgagcgg gtactgacca cggtcatgtt caccgacatc 2581 gtcggctcga cccagcgcgc agccaccgtg ggtgacctgc gctggcgcga cctgctcgat 2641 aaccacgaca acctggtccg ccacgagatc cagcggttcg gtgggcgcga ggtcaatacc 2701 gcgggcgacg ggttcgtcgc gacgttcagc agcccgagtg cggcaatcaa ctgcgcggac 2761 gccgtcgtcg acgccgtcgc cgtgctcggc atcgaggtcc gggtggggat tcatgccggc 2821 gaggtcgagg tgcggggggc cgacgtcgcc ggcctggccg tacacatcgg tgcccgggtc 2881 tgtgcgctgg ccggctccag tgaggtgctg gtgtcctcga cggtgcgtga catcgtcacc 2941 ggctcgagcc acagattcgc cgagcgcggc gaacacgaac tcaagggcgt gccgggccga 3001 tggcggcttt gtgcgctggt ccgggagcac ctcacgggcc agcggtagta ctagagatta 3061 aagaggagaa atactagatg gacggctatg tgctggagct ccagaactgg acggccggaa 3121 tcctcaactg gcaccgggac tgccgccgtt acggagccgc cgatctggcc cggcgcgcgc 3181 atggcttcgt gccggggcag ttgccgaacc ttttcagccg gaataaggga ttcgtggaac 3241 ccgcgtgggg cgtggtgagt cctgtggggt gaaggcggcc actgaccggc cgcaccacgg 3301 gggacgcaac acggaacacg ggggtgttct caccatgacc gacatcatcg agaggatcac 3361 ggacggcctg gcgggcgcgg cgcgggatgc cgcgcccgcc gcggcggacg gtgaactgac 3421 gccgtacgcg gcgccgttga ccgttcctcc cgtcctgcgt cccgactcca cggacgtcct 3481 gcgggagacc gagatcgcac tgcggccgac gtgggtgcgg ctgcacccgc agctcccgcc 3541 gacactcatg tgggggtacg gcggatcggt gccggggccg accgtcgagg tccggcgcgg 3601 gcagcgcgtc cgcatcgcct ggaccaaccg catcccgaag ggcagcgagt acccggtcac 3661 cgccgtcgag gtgcccgcgg cgaaccccaa cccgagcacc gaaccgggcc gcggcggcgt 3721 cccgcccatc gcggacgtcg ccgccctgcc cgcctggacc gtcacccacc tgcacggcgc 3781 gcagacgggc ggcggcaacg acggctgggc ggacaacgcg gtgggctacg gcgacgccca 3841 gctctccgag tacccgaacg accaccaggc ggtccagtgg tggtaccacg accatgccat 3901 gaacgtgacg cggtggaacg tccacacggg cctctacggc acctatctcg tccgcgacga 3961 cgaggaggac gcgctccaac tcccctccgg gaaaagggag atcccgctcc tcatcgccga 4021 ccgcaacctc gacacggacg aggacggcgc gctcaacggc cggctgctgc acaagaccgt 4081 gatcgtccag gagaagaacc cggagacggg caagccggtc tccgtgccct tcgcgggccc 4141 gtacaccacg gtcaacggcc gtatctggcc ctacgccgag gtggacgcgg cctggcaccg 4201 cttccgcctg gtcaacgcgt cgaacgccag gatctacgac ctcgtcctga tcgacgagga 4261 cgacaacccg gtgccgggcg tcctgcatca gatcggcagc gacggcggcc ttctcccgcg 4321 cccggtgccg gtcgacttcg acgaggcgct gcccacactg accgtcgccc cggccgagcg 4381 catggacctg ctcatcgact tccgcgcgct cgccggacgc cgcctacgcc tcgtcaacaa 4441 gggcgcgggc caggcgcccg gcgtgcccga cccggcgaac aacgtgcgct atccgcacgt 4501 catggagttc cgcgtcggtg agtgcggcac ggacgacccg ttcgagctgc ccgaggtcct 4561 ctcgggctcc ttccgccggc tgacgcacga catcgagcac gggcaccgcc tcatcgtgct 4621 gaccccgccg gccacgaagg gcggcggcgg ccaccccgag atctgggaga tgaccgaggt 4681 cgaggacgcc gaacaggtcg agctgcccgc ggacggggtc atccaactca tgagctccga 4741 aggcgcgttg aagacgtacc gccgcacctc acgcaccttc aacgacggcc tcggcttcac 4801 cgtcgcggag ggcagttacg agcagtggag cttcttgaac ctcgccgtga acccgcccgt 4861 cgtgcacccc atgcacatcc atctcgccga cttccagatc ctcggccgtg acacgtacga 4921 cgtgtcgggg ttcgacgtga cggccggcgg cacgcgcgcg ccgctcgcgc ccgacccggc 4981 cacgccggtc ccgctcccgc cgaacgagcg cggctacaag gacgtcttca gggcgctgcc 5041 aggccagatg ctgcgcgtca tgggacggtt cgacggcgcc tacgggcgct tcatgtacca 5101 ctgccatctg ctggagcacg aggacatggg catgatgcgg ccgttcgtgg tgatgcccgc 5161 cgaggcgatg aagttcgacc acggggcggg ccacggcgga catgggggac acggagggca 5221 cggcggctga ccggacgcca gaaatgcccg gttcagtctc actcgttcgg gaatcgtcgc 5281 gccggacgcg gacgggtaga gctttggccg gaggtgggcc atggaacaga ccgcgttgcg 5341 ccccaagtcg atgcccggag ctccgcgtcg tgatactaga gaaaaaaaaa ccccgcccct 5401 gacagggcgg ggtttttttt //