BBa_M1112 1 BBa_M1112 Lignin Peroxidase gene from Mycobacterium marinum 2011-04-09T11:00:00Z 2015-05-08T01:13:53Z M. marinum This gene sequence will code for a lignin peroxidase which catalyzes the oxidation of phenolic and non-phenolic compounds and aromatic amines, aromatic ethers and polycyclic aromatic hydrocarbons. false false _768_ 0 8952 9 Not in stock false Secretion tag may need to be incorporated for secretion and proper function. false Joshua Ellis annotation2115812 1 coding region range2115812 1 4 1374 annotation2115810 1 start codon range2115810 1 1 3 annotation2115811 1 stop codon range2115811 1 1375 1377 BBa_B0030 1 BBa_B0030 RBS.1 (strong) -- modified from R. Weiss 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 Strong RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>. false true _44_46_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix (&quot;orig&quot; in figure 4-14 of Ron Weiss thesis). <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation1701 1 RBS-1\Strong range1701 1 1 15 annotation7025 1 BBa_B0030 range7025 1 1 15 annotation1702 1 RBS range1702 1 8 12 BBa_M1130 1 BBa_M1130 Phlebia sp. b19, manganese peroxidase 2011-04-11T11:00:00Z 2015-05-08T01:13:53Z Phlebia sp. b19 Manganese peroxidase false false _768_ 0 9065 9 Not in stock false none false Meredith Halling annotation2115834 1 start codon range2115834 1 1 3 BBa_J23110 1 BBa_J23110 constitutive promoter family member 2006-08-16T11:00:00Z 2015-08-31T04:08:40Z Later Later false true _52_ 0 483 95 In stock true N/A true John Anderson BBa_M1115 1 BBa_M1115 Final lignin degradation construct 2011-04-16T11:00:00Z 2015-05-08T01:13:53Z Promoter, RBS, and terminator parts came from the registry. The coding sequences came from genomic sequences found within the NCBI and JGI databases. This composite part provides all the functions necessary to produce extracellular lignin degrading enzymes. false false _768_ 0 8952 9 Not in stock false None false Joshua Ellis component2116101 1 BBa_B0030 component2116111 1 BBa_M1112 component2116099 1 BBa_J23110 component2116104 1 BBa_M1130 component2116106 1 BBa_B0030 component2116123 1 BBa_B1006 component2116113 1 BBa_B0030 component2116118 1 BBa_M1113 annotation2116106 1 BBa_B0030 range2116106 1 1649 1663 annotation2116104 1 BBa_M1130 range2116104 1 65 1640 annotation2116123 1 BBa_B1006 range2116123 1 5382 5420 annotation2116111 1 BBa_M1112 range2116111 1 1672 3048 annotation2116113 1 BBa_B0030 range2116113 1 3057 3071 annotation2116118 1 BBa_M1113 range2116118 1 3078 5373 annotation2116101 1 BBa_B0030 range2116101 1 44 58 annotation2116099 1 BBa_J23110 range2116099 1 1 35 BBa_M1113 1 BBa_M1113 Laccase gene from Streptomyces lavendulae 2011-04-09T11:00:00Z 2015-05-08T01:13:53Z S. lavendulae Laccase directly oxidizes phenolic subunits only, which comprises ~10% of the total polymer in natural lignin. false false _768_ 0 8952 9 Not in stock false May need to add or change secretion tag to work properly with your host. false Joshua Ellis annotation2115815 1 stop codon range2115815 1 2294 2296 annotation2115814 1 start codon range2115814 1 1 3 annotation2115813 1 coding region range2115813 1 1 2296 BBa_B1006 1 BBa_B1006 Terminator (artificial, large, %T~>90) 2006-08-30T11:00:00Z 2015-08-31T04:07:21Z modified E. coli thr terminator, replaced all A-T pairs in stem with C-G pairs Released HQ 2013 Artificial terminator, estimated %T~>90% *8bp stem, 6nt loop *Bidirectional, estimated reverse %T~>90% false true _41_ 0 745 41 In stock false Bidirectional, with the reverse estimated to be less effective than the forward. Has a polyA tail of 9 residues. true Haiyao Huang annotation1898431 1 PolyA range1898431 1 1 9 annotation1898429 1 modified thr terminator range1898429 1 10 31 annotation1898430 1 PolyA range1898430 1 32 39 annotation1898428 1 B1006 range1898428 1 1 39 BBa_M1130_sequence 1 atggctttcaactttgctgcaatcctcgcctttgtctcactcgccgccgtgacgagtgctgccccgagcgggaccacctgttccaatggtgtagtcgttcctgacgcagtgtgctgcgacttcgtcccagtaaggcattctgctcctgtctttttatgcagcacactgaatcgtcgtacgaaatagcttgcatctgcccttcagagcgaggtcctcatgggcgactgtggtgaagacggtaggccaagttcctgtcatcctatccctgaaccgctgtctcacttgctctccagcgcacgagctcgtccgtcttattttccgtaagtctggctcgtcaatcgtctccggtattgtattgacactatgttagacgatgccattgccatctcccaaagcatgggtccctcagcgtaagtgaaaactctcgttaaaattggtttgtgctgatttgactcggcctacagtggtggcggagccgacggctcgatgctcatcttccctacggtcgaaccggcgttcttcccgaaccttggtatcgccgacagcgtcaacaacttgatccccttcctctcacaattccccacgatttctgctggtgatcttgttcagttcgcaggcgccgtcgcgatcagcaactgccccgtaagcagctacagcgctcggtcttctactgaaccgatgctgacacgaattgtgcatacagggcgcccctcaactggagttccttgccggacgttccaacgcgaccgctcctgccatcgacggcctcatccccgagccccaggacgatgtcaccaagattcttgcgcgctttgcggatgctggcaacttcacccctgacgaagtcgttgctctcctggcttcgcacagtatcgcccgtgctgatcacgtcgacccgacgcttgacgccgcgcccttcgactccgtgagtggtgcctattgcccgcccctctttcaccggctgacttcatctaccatcccacagacgccgttcgacttcgacacccaggtcttccttgaggtgctgctcaagggcaccggcttccccggcctggacaacaacaccggcgaggtctcctccccgctccccgtcaccgatggcaccgatgtcggcgagctgcgcctccagtccgacttcgtgcttgcccgcgacgagcgcaccgcctgcgcctggcagagcttcgtcaacgaacagcaggcgatggctgatgcgttcaaggaggcagtcaagaagctcgccgtgctcggccacagccgcagcgacctcgtcgactgctccgcggtcgtccctgtccccaagcccgcgactggcacgcccgccacgttccccgcttcgacgggcccgcaggacctcgagctcacgtgcacgaccgtgccgttccccacgctgtctactgcccgtaagtctcgctgttttctctcatctatgatctgcaccgttgctaatcgcccactcttcatcacagccggcgcccagcagactctgatcccgcactgctccgacggcaccatgacttgcaactccgtgcagttcgacggccccgccaccaacttcggtggtgcagacgactcgtag BBa_M1112_sequence 1 gtgtcgcaggcgccccgaactccccggactcgctacgccaggtgcggcgacctggacatcgcctaccaggtgatcggggatcatccgatcgacctgctcgtcataccgggggcctcgattccggtcgacaccatcgattccgaaccgtcgatgtaccgctttcaccgccgtctggcgtcgttttcccgcctgatccggttcgaccatcgcggcgtcggcctgtcatcgcgggtttcgtcgccggatgcgctcggcccgaggttctgggccgaggatgcgattgcggtgatggacgcggccggatgccaggaagccacgattcttgcctccgggttcaccgcgacgaccgccctggtgctggccgccgactatcccgagcgggtgcgcagcctggtgctcatcaacgcctccgcgcggacactgcatgcgcccgactacgaactgggcatcaggtccaacaccgccgaaccattcctcaccctcggaaccgatcccgacgcggtcgagcaggggttcgacgtgctgcgcatcatggcgcccagcgtggcccatgacgacgcgttccgcgactggtgggatctggccggaaaccgggcggcctcgcccagcacggcgcgcgccttcatcaacgcggttcaagcctcggatgcgcgcgactcgctgccccacatcaccgcgccgacgctgatcctgcatcgggtgggcaccaagttcgttccggtcgagcacggccgctatctggccgagcacattgccgggtcgcgcttggtcgagcttcccggttccgattccttgtattgggtcggcgacaccgccgcgttgctcgacgaggtcgaggagttcatcaccggcgtccggggcggcttcgtcaccgagcgggtactgaccacggtcatgttcaccgacatcgtcggctcgacccagcgcgcagccaccgtgggtgacctgcgctggcgcgacctgctcgataaccacgacaacctggtccgccacgagatccagcggttcggtgggcgcgaggtcaataccgcgggcgacgggttcgtcgcgacgttcagcagcccgagtgcggcaatcaactgcgcggacgccgtcgtcgacgccgtcgccgtgctcggcatcgaggtccgggtggggattcatgccggcgaggtcgaggtgcggggggccgacgtcgccggcctggccgtacacatcggtgcccgggtctgtgcgctggccggctccagtgaggtgctggtgtcctcgacggtgcgtgacatcgtcaccggctcgagccacagattcgccgagcgcggcgaacacgaactcaagggcgtgccgggccgatggcggctttgtgcgctggtccgggagcacctcacgggccagcggtag BBa_M1115_sequence 1 tttacggctagctcagtcctaggtacaatgctagctactagagattaaagaggagaaatactagatggctttcaactttgctgcaatcctcgcctttgtctcactcgccgccgtgacgagtgctgccccgagcgggaccacctgttccaatggtgtagtcgttcctgacgcagtgtgctgcgacttcgtcccagtaaggcattctgctcctgtctttttatgcagcacactgaatcgtcgtacgaaatagcttgcatctgcccttcagagcgaggtcctcatgggcgactgtggtgaagacggtaggccaagttcctgtcatcctatccctgaaccgctgtctcacttgctctccagcgcacgagctcgtccgtcttattttccgtaagtctggctcgtcaatcgtctccggtattgtattgacactatgttagacgatgccattgccatctcccaaagcatgggtccctcagcgtaagtgaaaactctcgttaaaattggtttgtgctgatttgactcggcctacagtggtggcggagccgacggctcgatgctcatcttccctacggtcgaaccggcgttcttcccgaaccttggtatcgccgacagcgtcaacaacttgatccccttcctctcacaattccccacgatttctgctggtgatcttgttcagttcgcaggcgccgtcgcgatcagcaactgccccgtaagcagctacagcgctcggtcttctactgaaccgatgctgacacgaattgtgcatacagggcgcccctcaactggagttccttgccggacgttccaacgcgaccgctcctgccatcgacggcctcatccccgagccccaggacgatgtcaccaagattcttgcgcgctttgcggatgctggcaacttcacccctgacgaagtcgttgctctcctggcttcgcacagtatcgcccgtgctgatcacgtcgacccgacgcttgacgccgcgcccttcgactccgtgagtggtgcctattgcccgcccctctttcaccggctgacttcatctaccatcccacagacgccgttcgacttcgacacccaggtcttccttgaggtgctgctcaagggcaccggcttccccggcctggacaacaacaccggcgaggtctcctccccgctccccgtcaccgatggcaccgatgtcggcgagctgcgcctccagtccgacttcgtgcttgcccgcgacgagcgcaccgcctgcgcctggcagagcttcgtcaacgaacagcaggcgatggctgatgcgttcaaggaggcagtcaagaagctcgccgtgctcggccacagccgcagcgacctcgtcgactgctccgcggtcgtccctgtccccaagcccgcgactggcacgcccgccacgttccccgcttcgacgggcccgcaggacctcgagctcacgtgcacgaccgtgccgttccccacgctgtctactgcccgtaagtctcgctgttttctctcatctatgatctgcaccgttgctaatcgcccactcttcatcacagccggcgcccagcagactctgatcccgcactgctccgacggcaccatgacttgcaactccgtgcagttcgacggccccgccaccaacttcggtggtgcagacgactcgtagtactagagattaaagaggagaaatactagaggtgtcgcaggcgccccgaactccccggactcgctacgccaggtgcggcgacctggacatcgcctaccaggtgatcggggatcatccgatcgacctgctcgtcataccgggggcctcgattccggtcgacaccatcgattccgaaccgtcgatgtaccgctttcaccgccgtctggcgtcgttttcccgcctgatccggttcgaccatcgcggcgtcggcctgtcatcgcgggtttcgtcgccggatgcgctcggcccgaggttctgggccgaggatgcgattgcggtgatggacgcggccggatgccaggaagccacgattcttgcctccgggttcaccgcgacgaccgccctggtgctggccgccgactatcccgagcgggtgcgcagcctggtgctcatcaacgcctccgcgcggacactgcatgcgcccgactacgaactgggcatcaggtccaacaccgccgaaccattcctcaccctcggaaccgatcccgacgcggtcgagcaggggttcgacgtgctgcgcatcatggcgcccagcgtggcccatgacgacgcgttccgcgactggtgggatctggccggaaaccgggcggcctcgcccagcacggcgcgcgccttcatcaacgcggttcaagcctcggatgcgcgcgactcgctgccccacatcaccgcgccgacgctgatcctgcatcgggtgggcaccaagttcgttccggtcgagcacggccgctatctggccgagcacattgccgggtcgcgcttggtcgagcttcccggttccgattccttgtattgggtcggcgacaccgccgcgttgctcgacgaggtcgaggagttcatcaccggcgtccggggcggcttcgtcaccgagcgggtactgaccacggtcatgttcaccgacatcgtcggctcgacccagcgcgcagccaccgtgggtgacctgcgctggcgcgacctgctcgataaccacgacaacctggtccgccacgagatccagcggttcggtgggcgcgaggtcaataccgcgggcgacgggttcgtcgcgacgttcagcagcccgagtgcggcaatcaactgcgcggacgccgtcgtcgacgccgtcgccgtgctcggcatcgaggtccgggtggggattcatgccggcgaggtcgaggtgcggggggccgacgtcgccggcctggccgtacacatcggtgcccgggtctgtgcgctggccggctccagtgaggtgctggtgtcctcgacggtgcgtgacatcgtcaccggctcgagccacagattcgccgagcgcggcgaacacgaactcaagggcgtgccgggccgatggcggctttgtgcgctggtccgggagcacctcacgggccagcggtagtactagagattaaagaggagaaatactagatggacggctatgtgctggagctccagaactggacggccggaatcctcaactggcaccgggactgccgccgttacggagccgccgatctggcccggcgcgcgcatggcttcgtgccggggcagttgccgaaccttttcagccggaataagggattcgtggaacccgcgtggggcgtggtgagtcctgtggggtgaaggcggccactgaccggccgcaccacgggggacgcaacacggaacacgggggtgttctcaccatgaccgacatcatcgagaggatcacggacggcctggcgggcgcggcgcgggatgccgcgcccgccgcggcggacggtgaactgacgccgtacgcggcgccgttgaccgttcctcccgtcctgcgtcccgactccacggacgtcctgcgggagaccgagatcgcactgcggccgacgtgggtgcggctgcacccgcagctcccgccgacactcatgtgggggtacggcggatcggtgccggggccgaccgtcgaggtccggcgcgggcagcgcgtccgcatcgcctggaccaaccgcatcccgaagggcagcgagtacccggtcaccgccgtcgaggtgcccgcggcgaaccccaacccgagcaccgaaccgggccgcggcggcgtcccgcccatcgcggacgtcgccgccctgcccgcctggaccgtcacccacctgcacggcgcgcagacgggcggcggcaacgacggctgggcggacaacgcggtgggctacggcgacgcccagctctccgagtacccgaacgaccaccaggcggtccagtggtggtaccacgaccatgccatgaacgtgacgcggtggaacgtccacacgggcctctacggcacctatctcgtccgcgacgacgaggaggacgcgctccaactcccctccgggaaaagggagatcccgctcctcatcgccgaccgcaacctcgacacggacgaggacggcgcgctcaacggccggctgctgcacaagaccgtgatcgtccaggagaagaacccggagacgggcaagccggtctccgtgcccttcgcgggcccgtacaccacggtcaacggccgtatctggccctacgccgaggtggacgcggcctggcaccgcttccgcctggtcaacgcgtcgaacgccaggatctacgacctcgtcctgatcgacgaggacgacaacccggtgccgggcgtcctgcatcagatcggcagcgacggcggccttctcccgcgcccggtgccggtcgacttcgacgaggcgctgcccacactgaccgtcgccccggccgagcgcatggacctgctcatcgacttccgcgcgctcgccggacgccgcctacgcctcgtcaacaagggcgcgggccaggcgcccggcgtgcccgacccggcgaacaacgtgcgctatccgcacgtcatggagttccgcgtcggtgagtgcggcacggacgacccgttcgagctgcccgaggtcctctcgggctccttccgccggctgacgcacgacatcgagcacgggcaccgcctcatcgtgctgaccccgccggccacgaagggcggcggcggccaccccgagatctgggagatgaccgaggtcgaggacgccgaacaggtcgagctgcccgcggacggggtcatccaactcatgagctccgaaggcgcgttgaagacgtaccgccgcacctcacgcaccttcaacgacggcctcggcttcaccgtcgcggagggcagttacgagcagtggagcttcttgaacctcgccgtgaacccgcccgtcgtgcaccccatgcacatccatctcgccgacttccagatcctcggccgtgacacgtacgacgtgtcggggttcgacgtgacggccggcggcacgcgcgcgccgctcgcgcccgacccggccacgccggtcccgctcccgccgaacgagcgcggctacaaggacgtcttcagggcgctgccaggccagatgctgcgcgtcatgggacggttcgacggcgcctacgggcgcttcatgtaccactgccatctgctggagcacgaggacatgggcatgatgcggccgttcgtggtgatgcccgccgaggcgatgaagttcgaccacggggcgggccacggcggacatgggggacacggagggcacggcggctgaccggacgccagaaatgcccggttcagtctcactcgttcgggaatcgtcgcgccggacgcggacgggtagagctttggccggaggtgggccatggaacagaccgcgttgcgccccaagtcgatgcccggagctccgcgtcgtgatactagagaaaaaaaaaccccgcccctgacagggcggggtttttttt BBa_M1113_sequence 1 atggacggctatgtgctggagctccagaactggacggccggaatcctcaactggcaccgggactgccgccgttacggagccgccgatctggcccggcgcgcgcatggcttcgtgccggggcagttgccgaaccttttcagccggaataagggattcgtggaacccgcgtggggcgtggtgagtcctgtggggtgaaggcggccactgaccggccgcaccacgggggacgcaacacggaacacgggggtgttctcaccatgaccgacatcatcgagaggatcacggacggcctggcgggcgcggcgcgggatgccgcgcccgccgcggcggacggtgaactgacgccgtacgcggcgccgttgaccgttcctcccgtcctgcgtcccgactccacggacgtcctgcgggagaccgagatcgcactgcggccgacgtgggtgcggctgcacccgcagctcccgccgacactcatgtgggggtacggcggatcggtgccggggccgaccgtcgaggtccggcgcgggcagcgcgtccgcatcgcctggaccaaccgcatcccgaagggcagcgagtacccggtcaccgccgtcgaggtgcccgcggcgaaccccaacccgagcaccgaaccgggccgcggcggcgtcccgcccatcgcggacgtcgccgccctgcccgcctggaccgtcacccacctgcacggcgcgcagacgggcggcggcaacgacggctgggcggacaacgcggtgggctacggcgacgcccagctctccgagtacccgaacgaccaccaggcggtccagtggtggtaccacgaccatgccatgaacgtgacgcggtggaacgtccacacgggcctctacggcacctatctcgtccgcgacgacgaggaggacgcgctccaactcccctccgggaaaagggagatcccgctcctcatcgccgaccgcaacctcgacacggacgaggacggcgcgctcaacggccggctgctgcacaagaccgtgatcgtccaggagaagaacccggagacgggcaagccggtctccgtgcccttcgcgggcccgtacaccacggtcaacggccgtatctggccctacgccgaggtggacgcggcctggcaccgcttccgcctggtcaacgcgtcgaacgccaggatctacgacctcgtcctgatcgacgaggacgacaacccggtgccgggcgtcctgcatcagatcggcagcgacggcggccttctcccgcgcccggtgccggtcgacttcgacgaggcgctgcccacactgaccgtcgccccggccgagcgcatggacctgctcatcgacttccgcgcgctcgccggacgccgcctacgcctcgtcaacaagggcgcgggccaggcgcccggcgtgcccgacccggcgaacaacgtgcgctatccgcacgtcatggagttccgcgtcggtgagtgcggcacggacgacccgttcgagctgcccgaggtcctctcgggctccttccgccggctgacgcacgacatcgagcacgggcaccgcctcatcgtgctgaccccgccggccacgaagggcggcggcggccaccccgagatctgggagatgaccgaggtcgaggacgccgaacaggtcgagctgcccgcggacggggtcatccaactcatgagctccgaaggcgcgttgaagacgtaccgccgcacctcacgcaccttcaacgacggcctcggcttcaccgtcgcggagggcagttacgagcagtggagcttcttgaacctcgccgtgaacccgcccgtcgtgcaccccatgcacatccatctcgccgacttccagatcctcggccgtgacacgtacgacgtgtcggggttcgacgtgacggccggcggcacgcgcgcgccgctcgcgcccgacccggccacgccggtcccgctcccgccgaacgagcgcggctacaaggacgtcttcagggcgctgccaggccagatgctgcgcgtcatgggacggttcgacggcgcctacgggcgcttcatgtaccactgccatctgctggagcacgaggacatgggcatgatgcggccgttcgtggtgatgcccgccgaggcgatgaagttcgaccacggggcgggccacggcggacatgggggacacggagggcacggcggctgaccggacgccagaaatgcccggttcagtctcactcgttcgggaatcgtcgcgccggacgcggacgggtagagctttggccggaggtgggccatggaacagaccgcgttgcgccccaagtcgatgcccggagctccgcgtcgtga BBa_B0030_sequence 1 attaaagaggagaaa BBa_B1006_sequence 1 aaaaaaaaaccccgcccctgacagggcggggtttttttt BBa_J23110_sequence 1 tttacggctagctcagtcctaggtacaatgctagc igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z