LOCUS       BBa_M11521               952 bp    DNA     linear       12-Jun-2024
DEFINITION  Zinc Promoter (ZntR regulated) with GFP Reporter
ACCESSION   BBa_M11521
VERSION     BBa_M11521.1  
FEATURES             Location/Qualifiers
     promoter        35..69
                     /label=-35 and -10 region
     misc_feature    32..53
                     /label=Palindromic binding site for ZntR
     promoter        1..69
                     /label=BBa_K190016
     misc_feature    82..85
                     /label=conserved
     RBS             78..89
                     /label=BBa_B0034
     misc_feature    96..98
                     /label=Start
     CDS             96..815
                     /label=GFP Coding Region
     CDS             96..815
                     /label=BBa_K208000
     stem_loop       835..878
                     /label=stem_loop
     misc_feature    824..903
                     /label=BBa_B0010
     terminator      824..903
                     /label=BBa_B0010
     stem_loop       919..938
                     /label=T7 TE
     misc_feature    945..945
                     /label=stop
     polyA_site      939..952
                     /label=polya
     misc_feature    912..952
                     /label=BBa_B0012
     terminator      912..952
                     /label=BBa_B0012
ORIGIN
        1 cgtccgctcg ctgtatctct gataaaactt gactctggag tcgactccag agtgtatcct
       61 tcggttaatt actagagaaa gaggagaaat actagatggc tagcaaagga gaagaacttt
      121 tcactggagt tgtcccaatt cttgttgaat tagatggtga tgttaatggg cacaaatttt
      181 ctgtcagtgg agagggtgaa ggtgatgcta catacggaaa gcttaccctt aaatttattt
      241 gcactactgg aaaactacct gttccatggc caacacttgt cactactttc tcttatggtg
      301 ttcaatgctt ttcccgttat ccggatcata tgaaacggca tgactttttc aagagtgcca
      361 tgcccgaagg ttatgtacag gaacgcacta tatctttcaa agatgacggg aactacaaga
      421 cgcgtgctga agtcaagttt gaaggtgata cccttgttaa tcgtatcgag ttaaaaggta
      481 ttgattttaa agaagatgga aacattctcg gacacaaact cgagtacaac tataactcac
      541 acaatgtata catcacggca gacaaacaaa agaatggaat caaagctaac ttcaaaattc
      601 gccacaacat tgaagatgga tccgttcaac tagcagacca ttatcaacaa aatactccaa
      661 ttggcgatgg ccctgtcctt ttaccagaca accattacct gtcgacacaa tctgcccttt
      721 cgaaagatcc caacgaaaag cgtgaccaca tggtccttct tgagtttgta actgctgctg
      781 ggattacaca tggcatggat gagctctaca aataatacta gagccaggca tcaaataaaa
      841 cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct
      901 ctctactaga gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta ta
//