LOCUS       BBa_M11522               938 bp    DNA     linear       01-Jun-2024
DEFINITION  Zinc Promoter (ZntR regulated) with RFP Reporter
ACCESSION   BBa_M11522
VERSION     BBa_M11522.1  
FEATURES             Location/Qualifiers
     promoter        35..69
                     /label=-35 and -10 region
     misc_feature    32..53
                     /label=Palindromic binding site for ZntR
     promoter        1..69
                     /label=BBa_K190016
     misc_feature    82..85
                     /label=conserved
     RBS             78..89
                     /label=BBa_B0034
     CDS             96..770
                     /label=mrfp1
     misc_feature    777..801
                     /label=Help:Barcodes
     CDS             96..801
                     /label=BBa_E1010
     stem_loop       821..864
                     /label=stem_loop
     misc_feature    810..889
                     /label=BBa_B0010
     terminator      810..889
                     /label=BBa_B0010
     stem_loop       905..924
                     /label=T7 TE
     misc_feature    931..931
                     /label=stop
     polyA_site      925..938
                     /label=polya
     misc_feature    898..938
                     /label=BBa_B0012
     terminator      898..938
                     /label=BBa_B0012
ORIGIN
        1 cgtccgctcg ctgtatctct gataaaactt gactctggag tcgactccag agtgtatcct
       61 tcggttaatt actagagaaa gaggagaaat actagatggc ttcctccgaa gacgttatca
      121 aagagttcat gcgtttcaaa gttcgtatgg aaggttccgt taacggtcac gagttcgaaa
      181 tcgaaggtga aggtgaaggt cgtccgtacg aaggtaccca gaccgctaaa ctgaaagtta
      241 ccaaaggtgg tccgctgccg ttcgcttggg acatcctgtc cccgcagttc cagtacggtt
      301 ccaaagctta cgttaaacac ccggctgaca tcccggacta cctgaaactg tccttcccgg
      361 aaggtttcaa atgggaacgt gttatgaact tcgaagacgg tggtgttgtt accgttaccc
      421 aggactcctc cctgcaagac ggtgagttca tctacaaagt taaactgcgt ggtaccaact
      481 tcccgtccga cggtccggtt atgcagaaaa aaaccatggg ttgggaagct tccaccgaac
      541 gtatgtaccc ggaagacggt gctctgaaag gtgaaatcaa aatgcgtctg aaactgaaag
      601 acggtggtca ctacgacgct gaagttaaaa ccacctacat ggctaaaaaa ccggttcagc
      661 tgccgggtgc ttacaaaacc gacatcaaac tggacatcac ctcccacaac gaagactaca
      721 ccatcgttga acagtacgaa cgtgctgaag gtcgtcactc caccggtgct taataacgct
      781 gatagtgcta gtgtagatcg ctactagagc caggcatcaa ataaaacgaa aggctcagtc
      841 gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca
      901 cactggctca ccttcgggtg ggcctttctg cgtttata
//