LOCUS       BBa_M11532              1368 bp    DNA     linear       07-Jun-2024
DEFINITION  Lead promoter with RFP
ACCESSION   BBa_M11532
VERSION     BBa_M11532.1  
FEATURES             Location/Qualifiers
     promoter        1..94
                     /label=BBa_I721001
     misc_feature    107..110
                     /label=conserved
     RBS             103..114
                     /label=BBa_B0034
     CDS             121..519
                     /label=BBa_I721002
     CDS             526..1200
                     /label=mrfp1
     misc_feature    1207..1231
                     /label=Help:Barcodes
     CDS             526..1231
                     /label=BBa_E1010
     stem_loop       1251..1294
                     /label=stem_loop
     misc_feature    1240..1319
                     /label=BBa_B0010
     terminator      1240..1319
                     /label=BBa_B0010
     stem_loop       1335..1354
                     /label=T7 TE
     misc_feature    1361..1361
                     /label=stop
     polyA_site      1355..1368
                     /label=polya
     misc_feature    1328..1368
                     /label=BBa_B0012
     terminator      1328..1368
                     /label=BBa_B0012
ORIGIN
        1 ggttgcttcc tataaaaaac ttgactctat atctactaga ggttttctaa tgatggcatc
       61 cggggaaaac cttgtcaatg aagagcgatc tatgtactag agaaagagga gaaatactag
      121 atgatgcgga tcggtgaact gggcaagaag gcagattgct tggtgcagac cgtgcgcttt
      181 tacgagtcag aaggcttgct gcccgagcct gcacgtagcg agggcaactt caggctctat
      241 gacgaagtcc atttgcagcg cttgctgttc atccgccgct gccgggcgaa ggacatgacg
      301 ctggatgaga tccgtcaact gctgaactta cgggatcggc cagagttggg ctgcggcgag
      361 gtgaacgcgc tggtcgacgc tcatatcgcg caagtgcgga ccaagatgaa ggaattgcgc
      421 gccttggagc gcgagttaat ggatctgcga cgctcctgcg atagcgcccg aacctcgcgc
      481 gagtgcggca ttctcaacag cttggccgag cccgcctgat actagatggc ttcctccgaa
      541 gacgttatca aagagttcat gcgtttcaaa gttcgtatgg aaggttccgt taacggtcac
      601 gagttcgaaa tcgaaggtga aggtgaaggt cgtccgtacg aaggtaccca gaccgctaaa
      661 ctgaaagtta ccaaaggtgg tccgctgccg ttcgcttggg acatcctgtc cccgcagttc
      721 cagtacggtt ccaaagctta cgttaaacac ccggctgaca tcccggacta cctgaaactg
      781 tccttcccgg aaggtttcaa atgggaacgt gttatgaact tcgaagacgg tggtgttgtt
      841 accgttaccc aggactcctc cctgcaagac ggtgagttca tctacaaagt taaactgcgt
      901 ggtaccaact tcccgtccga cggtccggtt atgcagaaaa aaaccatggg ttgggaagct
      961 tccaccgaac gtatgtaccc ggaagacggt gctctgaaag gtgaaatcaa aatgcgtctg
     1021 aaactgaaag acggtggtca ctacgacgct gaagttaaaa ccacctacat ggctaaaaaa
     1081 ccggttcagc tgccgggtgc ttacaaaacc gacatcaaac tggacatcac ctcccacaac
     1141 gaagactaca ccatcgttga acagtacgaa cgtgctgaag gtcgtcactc caccggtgct
     1201 taataacgct gatagtgcta gtgtagatcg ctactagagc caggcatcaa ataaaacgaa
     1261 aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct
     1321 actagagtca cactggctca ccttcgggtg ggcctttctg cgtttata
//