LOCUS       BBa_M12069              2646 bp    DNA     linear       26-May-2024
DEFINITION  hin invertase under constitutive promoter pspv from Salmonella
ACCESSION   BBa_M12069
VERSION     BBa_M12069.1  
FEATURES             Location/Qualifiers
     RBS             1..15
                     /label=RBS-1\Strong
     RBS             8..12
                     /label=RBS
     misc_feature    1..15
                     /label=BBa_B0030
     RBS             1..15
                     /label=BBa_B0030
     stem_loop       35..78
                     /label=stem_loop
     misc_feature    24..103
                     /label=BBa_B0010
     terminator      24..103
                     /label=BBa_B0010
     promoter        112..2067
                     /label=BBa_K112707
     misc_feature    2143..2148
                     /label=Former SpeI site
     misc_feature    2145..2145
                     /label=Mutation to delete the SpeI site
     CDS             2074..2646
                     /label=Hin sequence
     primer_bind     2074..2076
                     /label=Fwd primer J31001
     primer_bind     2624..2643
                     /label=Rev primer J31001
     CDS             2074..2646
                     /label=BBa_J31000
ORIGIN
        1 attaaagagg agaaatacta gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga
       61 ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctctactaga gagatcctgt
      121 gatgtttggc gattgatcag atcgcacaat ccgggctgag ttccctttca gtgatctact
      181 attttgcgaa gctatttagt gcacactaat cgatttttca gacaaccttt ctcgcctggc
      241 gtgagttttc gttcgactga acctataaaa aggctctgca ttctgtcctg tagtgcgcca
      301 ggcctaataa cgcctcccgt ttaattccgg cccggtcgta catcggttct gttcatttat
      361 gggatcgcga ctttttcgtt gtaatgcttc aagagcgatt ttttgttctg cggtcaactc
      421 aacattcata gccatcagca tgatcctgat tatttttgaa accaagcatc ttcattgatc
      481 atgggtatac atcgttgtta tccagctata catcataaca ggtcaattaa atccactcag
      541 aaataaagtc agggtatgca taaaatctat cgcccataat cctatccagt aaccccatga
      601 ttagtaagaa ctaatcagtc tgtgcaaaaa caggtcaccg ccatcctgtt tttgcacatc
      661 aaaacatttt ttcaggatta ttctgaaaaa aaaaaggaga tattatggat ttcttgatta
      721 ataaaaaatt aaaaattttc ataacactga tggaaacagg ttccttcagt atcgcaacat
      781 cagtactgta tatcacccga accccgctga gcagggttat ttcagacctg gaaagagagc
      841 tgaaacaaag actctttata cggaagaatg gcactcttat cccaaccgaa tttgcacaaa
      901 ctatttatcg aaaagtaaaa tcccattata ttttcttaca tgcactggag caggaaatcg
      961 gacctacggg taaaacgaaa caactagaaa taatatttga cgaaatttat ccgggaagtt
     1021 taaaaaatct gatcatttca gcactgacca tttccggcca aaaaacaaat ataatgggga
     1081 gagccgttaa cagccaaata atagaagaac tgtgtcagac aaacaactgc attgttattt
     1141 ctgccagaaa ttattttcat cgggaatcgc ttgtctgccg gacatcagtg gagggtgggg
     1201 tcatgttatt tattcctaaa aaattctttc tctgcggcaa acctgatatc aacaggctgg
     1261 ccggaacacc tgtacttttt catgaggggg ctaaaaattt taatctggac accatatacc
     1321 atttttttga acagacacta ggtattacca accctgcatt cagttttgat aacgtcgatt
     1381 tgttcagttc actgtaccgg ttacaacaag ggctggcgat gttactcatc cccgtcagag
     1441 tctgtcgggc tctgggatta tcaacagatc acgcactgca catcaaaggc gtagcgctct
     1501 gtacctcctt gtattacccg accaagaaac gggagacacc agattatcgt aaagctataa
     1561 aactgataca gcaggaactg aaacagtcca ccttctgacc ttatgcagcg taagggccgc
     1621 aacacctgta ttcacggcat ttgccagatt cagattgtca gcaatcccca tcctccatag
     1681 cggtagttca ccgcggagca tggagtaaac cggctggtcg ccgtcaatct gacacagaat
     1741 cagtttgatg ctctggtgga ttacctaaaa catgggcatt aacgcgctgg ctcacgccac
     1801 tttactgaag aaactgaata acggtgacta tgacggcgca gcgaatgaat taattcctga
     1861 aatgggacca cgccagcggt caggttgttc ccggcctgac ccgacgccgg agcgctgaac
     1921 gttgcttatt cctgagttaa tttgttgtgc catctttgca caccgggaac cgcgattccg
     1981 cacagcagaa aaatagcaca taaataaact caatataagc cactcatttt ctggcaatac
     2041 aaaataattc ccctgcaaac attatcgtac tagatggcta ctattgggta tattcgggtg
     2101 tcaacaattg accaaaatat cgatttacag cgtaatgcgc ttaccagtgc aaattgtgac
     2161 cgcatttttg aagaccgtat cagtggcaag attgcaaacc gccccggcct gaaacgggcg
     2221 ttaaagtatg taaataaagg cgatactctt gtcgtctgga aattagacag actgggccgt
     2281 agcgtgaaaa atctggtggc gttaatatca gaattacatg aacgtggagc tcacttccat
     2341 tctttaaccg atagtattga taccagtagc gcgatggggc gattcttttt tcatgtaatg
     2401 tcagcactgg ccgagatgga gcgagaatta atcgtcgagc gaacccttgc cggactggct
     2461 gccgccagag cgcaaggacg actgggaggg cgccctcggg cgatcaacaa acatgaacag
     2521 gaacagatta gtcggctatt agagaaaggc catcctcggc agcaattagc tattattttt
     2581 ggtattggcg tatccacctt atacagatac tttccggcaa gcagtataaa aaaacgaatg
     2641 aattaa
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