LOCUS       BBa_M12071              2646 bp    DNA     linear       19-May-2024
DEFINITION  hin invertase under constitutive promoter pspv from Salmonella
ACCESSION   BBa_M12071
VERSION     BBa_M12071.1  
FEATURES             Location/Qualifiers
     promoter        1..1956
                     /label=BBa_K112707
     RBS             1965..1979
                     /label=RBS-1\Strong
     RBS             1972..1976
                     /label=RBS
     misc_feature    1965..1979
                     /label=BBa_B0030
     RBS             1965..1979
                     /label=BBa_B0030
     misc_feature    2055..2060
                     /label=Former SpeI site
     misc_feature    2057..2057
                     /label=Mutation to delete the SpeI site
     CDS             1986..2558
                     /label=Hin sequence
     primer_bind     1986..1988
                     /label=Fwd primer J31001
     primer_bind     2536..2555
                     /label=Rev primer J31001
     CDS             1986..2558
                     /label=BBa_J31000
     stem_loop       2578..2621
                     /label=stem_loop
     misc_feature    2567..2646
                     /label=BBa_B0010
     terminator      2567..2646
                     /label=BBa_B0010
ORIGIN
        1 agatcctgtg atgtttggcg attgatcaga tcgcacaatc cgggctgagt tccctttcag
       61 tgatctacta ttttgcgaag ctatttagtg cacactaatc gatttttcag acaacctttc
      121 tcgcctggcg tgagttttcg ttcgactgaa cctataaaaa ggctctgcat tctgtcctgt
      181 agtgcgccag gcctaataac gcctcccgtt taattccggc ccggtcgtac atcggttctg
      241 ttcatttatg ggatcgcgac tttttcgttg taatgcttca agagcgattt tttgttctgc
      301 ggtcaactca acattcatag ccatcagcat gatcctgatt atttttgaaa ccaagcatct
      361 tcattgatca tgggtataca tcgttgttat ccagctatac atcataacag gtcaattaaa
      421 tccactcaga aataaagtca gggtatgcat aaaatctatc gcccataatc ctatccagta
      481 accccatgat tagtaagaac taatcagtct gtgcaaaaac aggtcaccgc catcctgttt
      541 ttgcacatca aaacattttt tcaggattat tctgaaaaaa aaaaggagat attatggatt
      601 tcttgattaa taaaaaatta aaaattttca taacactgat ggaaacaggt tccttcagta
      661 tcgcaacatc agtactgtat atcacccgaa ccccgctgag cagggttatt tcagacctgg
      721 aaagagagct gaaacaaaga ctctttatac ggaagaatgg cactcttatc ccaaccgaat
      781 ttgcacaaac tatttatcga aaagtaaaat cccattatat tttcttacat gcactggagc
      841 aggaaatcgg acctacgggt aaaacgaaac aactagaaat aatatttgac gaaatttatc
      901 cgggaagttt aaaaaatctg atcatttcag cactgaccat ttccggccaa aaaacaaata
      961 taatggggag agccgttaac agccaaataa tagaagaact gtgtcagaca aacaactgca
     1021 ttgttatttc tgccagaaat tattttcatc gggaatcgct tgtctgccgg acatcagtgg
     1081 agggtggggt catgttattt attcctaaaa aattctttct ctgcggcaaa cctgatatca
     1141 acaggctggc cggaacacct gtactttttc atgagggggc taaaaatttt aatctggaca
     1201 ccatatacca tttttttgaa cagacactag gtattaccaa ccctgcattc agttttgata
     1261 acgtcgattt gttcagttca ctgtaccggt tacaacaagg gctggcgatg ttactcatcc
     1321 ccgtcagagt ctgtcgggct ctgggattat caacagatca cgcactgcac atcaaaggcg
     1381 tagcgctctg tacctccttg tattacccga ccaagaaacg ggagacacca gattatcgta
     1441 aagctataaa actgatacag caggaactga aacagtccac cttctgacct tatgcagcgt
     1501 aagggccgca acacctgtat tcacggcatt tgccagattc agattgtcag caatccccat
     1561 cctccatagc ggtagttcac cgcggagcat ggagtaaacc ggctggtcgc cgtcaatctg
     1621 acacagaatc agtttgatgc tctggtggat tacctaaaac atgggcatta acgcgctggc
     1681 tcacgccact ttactgaaga aactgaataa cggtgactat gacggcgcag cgaatgaatt
     1741 aattcctgaa atgggaccac gccagcggtc aggttgttcc cggcctgacc cgacgccgga
     1801 gcgctgaacg ttgcttattc ctgagttaat ttgttgtgcc atctttgcac accgggaacc
     1861 gcgattccgc acagcagaaa aatagcacat aaataaactc aatataagcc actcattttc
     1921 tggcaataca aaataattcc cctgcaaaca ttatcgtact agagattaaa gaggagaaat
     1981 actagatggc tactattggg tatattcggg tgtcaacaat tgaccaaaat atcgatttac
     2041 agcgtaatgc gcttaccagt gcaaattgtg accgcatttt tgaagaccgt atcagtggca
     2101 agattgcaaa ccgccccggc ctgaaacggg cgttaaagta tgtaaataaa ggcgatactc
     2161 ttgtcgtctg gaaattagac agactgggcc gtagcgtgaa aaatctggtg gcgttaatat
     2221 cagaattaca tgaacgtgga gctcacttcc attctttaac cgatagtatt gataccagta
     2281 gcgcgatggg gcgattcttt tttcatgtaa tgtcagcact ggccgagatg gagcgagaat
     2341 taatcgtcga gcgaaccctt gccggactgg ctgccgccag agcgcaagga cgactgggag
     2401 ggcgccctcg ggcgatcaac aaacatgaac aggaacagat tagtcggcta ttagagaaag
     2461 gccatcctcg gcagcaatta gctattattt ttggtattgg cgtatccacc ttatacagat
     2521 actttccggc aagcagtata aaaaaacgaa tgaattaata ctagagccag gcatcaaata
     2581 aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac
     2641 gctctc
//