LOCUS BBa_M1354 7114 bp DNA linear 28-May-2024 DEFINITION Complete pathway for 1-butanol ACCESSION BBa_M1354 VERSION BBa_M1354.1 FEATURES Location/Qualifiers promoter 4..45 /label=sigA promoter RBS 46..55 /label=putative rbs misc_feature 46..46 /label=+1 Transcription Start Point misc_feature 9..14 /label=-35 box misc_feature 30..35 /label=-10 box promoter 1..61 /label=BBa_K390018 misc_feature 68..70 /label=Start Codon misc_feature 742..742 /label=EcoRI site- changed t to c CDS 68..1081 /label=Coding Sequence misc_feature 1082..1084 /label=Stop Codon CDS 68..1084 /label=BBa_M1302 misc_feature 1..1084 /label=BBa_M1303 stem_loop 1100..1119 /label=T7 TE misc_feature 1126..1126 /label=stop polyA_site 1120..1133 /label=polya misc_feature 1093..1133 /label=BBa_B0012 terminator 1093..1133 /label=BBa_B0012 stem_loop 1154..1176 /label=stem_loop misc_feature 1142..1187 /label=BBa_B0011 terminator 1142..1187 /label=BBa_B0011 terminator 1093..1187 /label=BBa_B0014 promoter 1199..1240 /label=sigA promoter RBS 1241..1250 /label=putative rbs misc_feature 1241..1241 /label=+1 Transcription Start Point misc_feature 1204..1209 /label=-35 box misc_feature 1225..1230 /label=-10 box promoter 1196..1256 /label=BBa_K390018 misc_feature 1263..1265 /label=Start misc_feature 2046..2048 /label=Stop CDS 1263..2048 /label=ORF CDS 1263..2048 /label=BBa_M1312 misc_feature 1196..2048 /label=BBa_M1328 stem_loop 2064..2083 /label=T7 TE misc_feature 2090..2090 /label=stop polyA_site 2084..2097 /label=polya misc_feature 2057..2097 /label=BBa_B0012 terminator 2057..2097 /label=BBa_B0012 stem_loop 2118..2140 /label=stem_loop misc_feature 2106..2151 /label=BBa_B0011 terminator 2106..2151 /label=BBa_B0011 terminator 2057..2151 /label=BBa_B0014 promoter 2163..2204 /label=sigA promoter RBS 2205..2214 /label=putative rbs misc_feature 2205..2205 /label=+1 Transcription Start Point misc_feature 2168..2173 /label=-35 box misc_feature 2189..2194 /label=-10 box promoter 2160..2220 /label=BBa_K390018 misc_feature 2227..2229 /label=start misc_feature 3361..3363 /label=stop misc_feature 2279..2279 /label=point mutation CDS 2227..3363 /label=BBa_M1311 misc_feature 2160..3363 /label=BBa_M1313 stem_loop 3379..3398 /label=T7 TE misc_feature 3405..3405 /label=stop polyA_site 3399..3412 /label=polya misc_feature 3372..3412 /label=BBa_B0012 terminator 3372..3412 /label=BBa_B0012 stem_loop 3433..3455 /label=stem_loop misc_feature 3421..3466 /label=BBa_B0011 terminator 3421..3466 /label=BBa_B0011 terminator 3372..3466 /label=BBa_B0014 promoter 3478..3519 /label=sigA promoter RBS 3520..3529 /label=putative rbs misc_feature 3520..3520 /label=+1 Transcription Start Point misc_feature 3483..3488 /label=-35 box misc_feature 3504..3509 /label=-10 box promoter 3475..3535 /label=BBa_K390018 misc_feature 3542..3544 /label=start CDS 3542..4389 /label=cds CDS 3542..4390 /label=BBa_M1350 misc_feature 3475..4390 /label=BBa_M1352 stem_loop 4406..4425 /label=T7 TE misc_feature 4432..4432 /label=stop polyA_site 4426..4439 /label=polya misc_feature 4399..4439 /label=BBa_B0012 terminator 4399..4439 /label=BBa_B0012 stem_loop 4460..4482 /label=stem_loop misc_feature 4448..4493 /label=BBa_B0011 terminator 4448..4493 /label=BBa_B0011 terminator 4399..4493 /label=BBa_B0014 promoter 4505..4546 /label=sigA promoter RBS 4547..4556 /label=putative rbs misc_feature 4547..4547 /label=+1 Transcription Start Point misc_feature 4510..4515 /label=-35 box misc_feature 4531..4536 /label=-10 box promoter 4502..4562 /label=BBa_K390018 CDS 4571..5747 /label=BBa_M1351 misc_feature 4502..5747 /label=BBa_M1353 stem_loop 5763..5782 /label=T7 TE misc_feature 5789..5789 /label=stop polyA_site 5783..5796 /label=polya misc_feature 5756..5796 /label=BBa_B0012 terminator 5756..5796 /label=BBa_B0012 stem_loop 5817..5839 /label=stem_loop misc_feature 5805..5850 /label=BBa_B0011 terminator 5805..5850 /label=BBa_B0011 terminator 5756..5850 /label=BBa_B0014 promoter 5862..5903 /label=sigA promoter RBS 5904..5913 /label=putative rbs misc_feature 5904..5904 /label=+1 Transcription Start Point misc_feature 5867..5872 /label=-35 box misc_feature 5888..5893 /label=-10 box promoter 5859..5919 /label=BBa_K390018 misc_feature 5926..5928 /label=Start misc_feature 7009..7011 /label=Stop CDS 5926..7008 /label=AA sequence CDS 5926..7011 /label=BBa_M1397 misc_feature 5859..7011 /label=BBa_M1396 stem_loop 7027..7046 /label=T7 TE misc_feature 7053..7053 /label=stop polyA_site 7047..7060 /label=polya misc_feature 7020..7060 /label=BBa_B0012 terminator 7020..7060 /label=BBa_B0012 stem_loop 7081..7103 /label=stem_loop misc_feature 7069..7114 /label=BBa_B0011 terminator 7069..7114 /label=BBa_B0011 terminator 7020..7114 /label=BBa_B0014 ORIGIN 1 cctcaacttt gactgaccag ctaattttgt acacgactta ggagttaacg aggaaaacgg 61 ctactagatg gatttggccg aatataaggg tatctatgtc attgcggaac agtttgaagg 121 taaactccgg gatgtttctt tcgaactcct cggtcaagcc cggattctgg cggacaccat 181 tggcgatgag gtaggagcca tcttgatcgg caaggatgtt aagcccttgg cgcaagagtt 241 aattgcgcac ggggcccata aagtgtacgt gtacgatgat ccgcagcttg aacactataa 301 tactaccgct tatgcgaagg tcatttgcga cttttttcac gaagagaagc ccaatgtctt 361 tttggtgggc gcgaccaata tcggtaggga cctgggtcca cgggtggcca attccctgaa 421 aaccggtctg accgcggatt gtacacaatt aggcgttgac gacgacaaaa aaaccattgt 481 ttggacccga cccgcgctgg ggggcaatat catggccgaa attatttgtc ccgataatcg 541 gccacagatg ggaacagttc gtccccacgt gtttaagaag cccgaagctg accccagcgc 601 taccggtgaa gtcatcgaga aaaaagccaa cttgtccgac gccgacttta tgaccaaatt 661 tgtggaattg atcaaactcg gtggggaagg tgtgaaaatt gaagacgcag atgttattgt 721 ggccgggggc cgcggtatga actctgagga acccttcaaa acggggatct tgaaagaatg 781 cgccgatgtg ctcggtggtg cggtgggtgc gtctcgcgct gctgtggatg ctggatggat 841 tgatgctctg catcaagtgg gtcagacagg gaaaaccgtg ggccccaaga tctacattgc 901 gtgtgcaatc agtggggcta ttcagccctt ggccgggatg actggtagcg actgtatcat 961 cgcgattaat aaagacgaag atgcgcccat ctttaaggtt tgtgattatg ggattgttgg 1021 cgacgttttt aaagtcttgc cccttctcac tgaagccatt aagaaacaaa aagggattgc 1081 gtaatactag agtcacactg gctcaccttc gggtgggcct ttctgcgttt atatactaga 1141 gagagaatat aaaaagccag attattaatc cggctttttt attattttac tagagcctca 1201 actttgactg accagctaat tttgtacacg acttaggagt taacgaggaa aacggctact 1261 agatggagct taataatgtg atcttagaga aagaaggaaa agtggcggtc gtaactatta 1321 accggcccaa agcccttaat gcgctcaata gcgacacgct aaaggaaatg gattatgtga 1381 ttggggagat cgaaaacgat agcgaagtgc tcgcggtcat tctcaccggt gcgggggaaa 1441 aatcatttgt ggcaggggcg gatatttccg agatgaaaga aatgaatacc attgaaggcc 1501 gtaaatttgg tatcttgggg aataaagtat ttcggcgact tgaattgctg gaaaaaccag 1561 taatcgcagc tgtgaatggt ttcgcgcttg ggggaggctg tgaaatcgcc atgtcttgtg 1621 atatccgcat tgcctcgtca aacgcccgtt tcggtcagcc cgaagttggc ttaggaatta 1681 cgcccggctt tggcgggaca caacgcctga gccgcctcgt cggtatgggc atggccaaac 1741 aattgatttt tactgcccaa aacatcaagg cggatgaagc tctgcgcatt ggccttgtta 1801 acaaagtagt tgagccatct gagctaatga atacggcgaa agaaattgcg aataagatcg 1861 tatcaaatgc gccggtggca gtgaaactca gtaagcaggc cattaataga ggtatgcaat 1921 gcgatattga tactgcttta gcctttgaat ctgaagcgtt cggcgaatgt tttagcaccg 1981 aagatcaaaa agatgcaatg accgccttta tcgaaaaacg aaaaatcgaa ggttttaaaa 2041 atcgctagta ctagagtcac actggctcac cttcgggtgg gcctttctgc gtttatatac 2101 tagagagaga atataaaaag ccagattatt aatccggctt ttttattatt ttactagagc 2161 ctcaactttg actgaccagc taattttgta cacgacttag gagttaacga ggaaaacggc 2221 tactagatgg actttaacct cacacgcgaa caggaactgg tccgccaaat ggttcgggca 2281 ttcgctgaga acgaagtgaa accgatcgct gccgaaattg acgaaaccga gcggtttccc 2341 atggaaaacg ttaaaaaaat ggggcaatat ggtatgatgg gtatcccctt ttctaaagag 2401 tacggaggtg ctggggggga tgtgttgtcg tatatcatcg ccgtcgaaga actcagcaaa 2461 gtgtgtggca ctacgggggt catcttatcg gcccatacca gcctatgtgc aagcttaatc 2521 aatgaacacg gtaccgagga gcagaaacag aaatatttgg tgcccttagc caaaggtgaa 2581 aaaatcgggg cgtacgggtt aaccgaaccc aatgccggga cagacagtgg cgcccaacag 2641 acggtcgccg tcctagaagg tgatcactac gttattaacg ggtctaagat ttttatcacc 2701 aatggtgggg tagccgatac ctttgtcatt tttgcgatga cggaccgcac taaaggcacc 2761 aagggtatta gtgcgtttat tatcgaaaaa ggctttaaag gcttttccat tggtaaggtc 2821 gaacaaaaac taggtatccg tgcatcttcg accacagaac tcgtgtttga agatatgatc 2881 gtgccagtcg aaaacatgat tggcaaagag gggaagggct tcccgattgc tatgaaaacc 2941 ctcgacgggg gccgtattgg gattgcagcc caggctttag gcatcgccga aggtgccttt 3001 aatgaagctc gcgcgtatat gaaagaacgg aaacaattcg gccgcagtct cgataaattt 3061 caaggtctgg cctggatgat ggcggatatg gacgttgcca ttgaaagtgc ccgctatctc 3121 gtctataaag cggcctatct caaacaagca ggactcccct acacagtaga cgcggctcgc 3181 gccaaattac acgcggcgaa tgtcgctatg gatgtaacca ccaaagccgt gcaattattc 3241 ggtggttacg ggtataccaa ggattatccg gtggagcgga tgatgagaga tgcaaagatc 3301 accgaaatct atgaagggac gtcggaggtg caaaaattgg tgatctctgg taaaatcttt 3361 cggtactaga gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta tatactagag 3421 agagaatata aaaagccaga ttattaatcc ggctttttta ttattttact agagcctcaa 3481 ctttgactga ccagctaatt ttgtacacga cttaggagtt aacgaggaaa acggctacta 3541 gatgaaaaag gtatgtgtta taggtgcagg tactatgggt tcaggaattg ctcaggcatt 3601 tgcagctaaa ggatttgaag tagtattaag agatattaaa gatgaatttg ttgatagagg 3661 attagatttt atcaataaaa atctttctaa attagttaaa aaaggaaaga tagaagaagc 3721 tactaaagtt gaaatcttaa ctagaatttc cggaacagtt gaccttaata tggcagctga 3781 ttgcgattta gttatagaag cagctgttga aagaatggat attaaaaagc agatttttgc 3841 tgacttagac aatatatgca agccagaaac aattcttgca tcaaatacat catcactttc 3901 aataacagaa gtggcatcag caactaaaag acctgataag gttataggta tgcatttctt 3961 taatccagct cctgttatga agcttgtaga ggtaataaga ggaatagcta catcacaaga 4021 aacttttgat gcagttaaag agacatctat agcaatagga aaagatcctg tagaagtagc 4081 agaagcacca ggatttgttg taaatagaat attaatacca atgattaatg aagcagttgg 4141 tatattagca gaaggaatag cttcagtaga agacatagat aaagctatga aacttggagc 4201 taatcaccca atgggaccat tagaattagg tgattttata ggtcttgata tatgtcttgc 4261 tataatggat gttttatact cagaaactgg agattctaag tatagaccac atacattact 4321 taagaagtat gtaagagcag gatggcttgg aagaaaatca ggaaaaggtt tctacgatta 4381 ttcaaaataa tactagagtc acactggctc accttcgggt gggcctttct gcgtttatat 4441 actagagaga gaatataaaa agccagatta ttaatccggc ttttttatta ttttactaga 4501 gcctcaactt tgactgacca gctaattttg tacacgactt aggagttaac gaggaaaacg 4561 gctactagag aatgcgggac gtcgtgatcg tgagtgctgt gcgaacggcc atcggagctt 4621 atgggaaaac cctaaaagat gtccccgcca cagagctggg tgctatcgtc ataaaagagg 4681 ccgttcggcg ggccaacatt aaccccaacg agatcaatga agtcattttt ggtaatgtct 4741 tacaggcagg acttggccag aatccggccc gtcaagcggc agtcaaggcg ggcctgccct 4801 tggaaacgcc cgcctttacc atcaataaag tatgtggaag tggattgaga tctattagtt 4861 tggcggccca gattatcaaa gcaggagatg ccgatacgat cgttgtgggc ggcatggaaa 4921 atatgtctcg ctcgccctac cttattaaca accagcgctg gggtcaacgc atgggcgaca 4981 gcgaacttgt cgacgaaatg attaaagatg gtctctggga tgcctttaac ggttatcaca 5041 tgggggttac agcggaaaat atcgctgaac aatggaacat tacccgcgag gaacaagatg 5101 aattttcctt aatgtctcaa caaaaagcag aaaaagccat taagaatggg gagtttaaag 5161 acgaaatcgt ccccgtgctc attaaaacta aaaaaggtga aatcgtcttt gatcaagatg 5221 gattcccccg gtttggcaac accatcgaag ctttgcggaa gctcaaaccg atttttaaag 5281 aaaacgggac cgtcaccgct ggcaacgcgt ctggcttaaa tgatggcgcc gcggccttgg 5341 taattatgtc tgccgataag gcaaacgcgc tgggtattaa acccctcgcc aaaattacca 5401 gttacggctc ttatggtgtc gatccctcga tcatgggcta cggtgcattt tacgctacaa 5461 aagctgccct cgacaagatt aacttgaagc cggaagatct cgacctcatt gaggcgaatg 5521 aagcatatgc aagtcagtcc atcgctgtca cgcgggactt gaatctggac atgtctaaag 5581 tcaatgtgaa cggcggcgcc atcgcgttag gtcatcccat cggggcttct ggcgcgcgta 5641 ttctcgtcac gctcctttac gctatgcaaa agcgcgattc gaaaaaaggg ttagcgacgc 5701 tctgcatcgg gggtggacag ggaaccgctc tcgtcgtcga acgggactac tagagtcaca 5761 ctggctcacc ttcgggtggg cctttctgcg tttatatact agagagagaa tataaaaagc 5821 cagattatta atccggcttt tttattattt tactagagcc tcaactttga ctgaccagct 5881 aattttgtac acgacttagg agttaacgag gaaaacggct actagatgag ccaaacggtt 5941 cggggcgtga ttgcacgcca aaaaggtgaa cctgttgaat tagtcaatat tgtcgtcccc 6001 gatcccggcc ctggcgaagc cgtcgtcgat gtgactgcct gcggggtgtg tcacacagac 6061 ttaacttatc gcgaaggggg tatcaatgac gagtatcctt tcttgctggg ccacgaggct 6121 gccgggatta ttgaggcggt cggtcccggc gtgacagctg ttgaacccgg tgatttcgtc 6181 atcttaaact ggcgtgcggt ctgtgggcaa tgccgtgctt gtaaacgcgg ccggccacgt 6241 tactgttttg ataccttcaa tgcggaacaa aaaatgacgc tcacggatgg aaccgaactc 6301 actgccgccc taggcattgg ggcctttgcg gataagaccc tcgtgcattc cggccaatgt 6361 accaaggttg atcccgcggc agatccggct gtcgcaggtt tgctcggctg tggtgtcatg 6421 gcgggtttgg gcgctgcaat taacacaggg ggtgtgacac gggatgatac tgttgctgta 6481 attggctgtg gtggagtggg tgatgctgct attgcagggg ctgcacttgt tggtgccaaa 6541 cgcattattg ccgtggatac cgatgatacg aaattggatt gggctagaac gtttggcgca 6601 acccacactg tcaacgcacg cgaagtagat gtcgtacaag ccattggagg acttactgat 6661 ggttttggag ctgacgttgt cattgatgcg gtaggccgac cggaaacgta tcagcaagcc 6721 ttttacgcga gggacctagc cggtacggta gtacttgttg gggtcccgac tcccgatatg 6781 cgcctcgaca tgcctctcgt cgattttttc agtcatggtg gcgcgctcaa aagcagttgg 6841 tacggtgatt gccttcctga gagcgatttt cccactctta ttgatttgta cttgcagggc 6901 cgcttaccct tgcagcgctt tgtgtcggaa cgcatcggac ttgaagatgt cgaggaagcc 6961 tttcataaga tgcacggcgg taaggtcctc cggtctgtgg ttatgctctg atactagagt 7021 cacactggct caccttcggg tgggcctttc tgcgtttata tactagagag agaatataaa 7081 aagccagatt attaatccgg cttttttatt attt //