LOCUS BBa_M1357 9359 bp DNA linear 28-May-2024 DEFINITION 1-butanol production pathway in integration plasmid ACCESSION BBa_M1357 VERSION BBa_M1357.1 FEATURES Location/Qualifiers oriT 1..720 /label=BBa_M1355 CDS 835..1494 /label=CmR misc_feature 729..1520 /label=BBa_P1000 promoter 1532..1573 /label=sigA promoter RBS 1574..1583 /label=putative rbs misc_feature 1574..1574 /label=+1 Transcription Start Point misc_feature 1537..1542 /label=-35 box misc_feature 1558..1563 /label=-10 box promoter 1529..1589 /label=BBa_K390018 misc_feature 1596..1598 /label=Start Codon misc_feature 2270..2270 /label=EcoRI site- changed t to c CDS 1596..2609 /label=Coding Sequence misc_feature 2610..2612 /label=Stop Codon CDS 1596..2612 /label=BBa_M1302 misc_feature 1529..2612 /label=BBa_M1303 stem_loop 2628..2647 /label=T7 TE misc_feature 2654..2654 /label=stop polyA_site 2648..2661 /label=polya misc_feature 2621..2661 /label=BBa_B0012 terminator 2621..2661 /label=BBa_B0012 stem_loop 2682..2704 /label=stem_loop misc_feature 2670..2715 /label=BBa_B0011 terminator 2670..2715 /label=BBa_B0011 terminator 2621..2715 /label=BBa_B0014 promoter 2727..2768 /label=sigA promoter RBS 2769..2778 /label=putative rbs misc_feature 2769..2769 /label=+1 Transcription Start Point misc_feature 2732..2737 /label=-35 box misc_feature 2753..2758 /label=-10 box promoter 2724..2784 /label=BBa_K390018 misc_feature 2791..2793 /label=Start misc_feature 3574..3576 /label=Stop CDS 2791..3576 /label=ORF CDS 2791..3576 /label=BBa_M1312 misc_feature 2724..3576 /label=BBa_M1328 stem_loop 3592..3611 /label=T7 TE misc_feature 3618..3618 /label=stop polyA_site 3612..3625 /label=polya misc_feature 3585..3625 /label=BBa_B0012 terminator 3585..3625 /label=BBa_B0012 stem_loop 3646..3668 /label=stem_loop misc_feature 3634..3679 /label=BBa_B0011 terminator 3634..3679 /label=BBa_B0011 terminator 3585..3679 /label=BBa_B0014 promoter 3691..3732 /label=sigA promoter RBS 3733..3742 /label=putative rbs misc_feature 3733..3733 /label=+1 Transcription Start Point misc_feature 3696..3701 /label=-35 box misc_feature 3717..3722 /label=-10 box promoter 3688..3748 /label=BBa_K390018 misc_feature 3755..3757 /label=start misc_feature 4889..4891 /label=stop misc_feature 3807..3807 /label=point mutation CDS 3755..4891 /label=BBa_M1311 misc_feature 3688..4891 /label=BBa_M1313 stem_loop 4907..4926 /label=T7 TE misc_feature 4933..4933 /label=stop polyA_site 4927..4940 /label=polya misc_feature 4900..4940 /label=BBa_B0012 terminator 4900..4940 /label=BBa_B0012 stem_loop 4961..4983 /label=stem_loop misc_feature 4949..4994 /label=BBa_B0011 terminator 4949..4994 /label=BBa_B0011 terminator 4900..4994 /label=BBa_B0014 promoter 5006..5047 /label=sigA promoter RBS 5048..5057 /label=putative rbs misc_feature 5048..5048 /label=+1 Transcription Start Point misc_feature 5011..5016 /label=-35 box misc_feature 5032..5037 /label=-10 box promoter 5003..5063 /label=BBa_K390018 misc_feature 5070..5072 /label=start CDS 5070..5917 /label=cds CDS 5070..5918 /label=BBa_M1350 misc_feature 5003..5918 /label=BBa_M1352 stem_loop 5934..5953 /label=T7 TE misc_feature 5960..5960 /label=stop polyA_site 5954..5967 /label=polya misc_feature 5927..5967 /label=BBa_B0012 terminator 5927..5967 /label=BBa_B0012 stem_loop 5988..6010 /label=stem_loop misc_feature 5976..6021 /label=BBa_B0011 terminator 5976..6021 /label=BBa_B0011 terminator 5927..6021 /label=BBa_B0014 promoter 6033..6074 /label=sigA promoter RBS 6075..6084 /label=putative rbs misc_feature 6075..6075 /label=+1 Transcription Start Point misc_feature 6038..6043 /label=-35 box misc_feature 6059..6064 /label=-10 box promoter 6030..6090 /label=BBa_K390018 CDS 6099..7275 /label=BBa_M1351 misc_feature 6030..7275 /label=BBa_M1353 stem_loop 7291..7310 /label=T7 TE misc_feature 7317..7317 /label=stop polyA_site 7311..7324 /label=polya misc_feature 7284..7324 /label=BBa_B0012 terminator 7284..7324 /label=BBa_B0012 stem_loop 7345..7367 /label=stem_loop misc_feature 7333..7378 /label=BBa_B0011 terminator 7333..7378 /label=BBa_B0011 terminator 7284..7378 /label=BBa_B0014 promoter 7390..7431 /label=sigA promoter RBS 7432..7441 /label=putative rbs misc_feature 7432..7432 /label=+1 Transcription Start Point misc_feature 7395..7400 /label=-35 box misc_feature 7416..7421 /label=-10 box promoter 7387..7447 /label=BBa_K390018 misc_feature 7454..7456 /label=Start misc_feature 8537..8539 /label=Stop CDS 7454..8536 /label=AA sequence CDS 7454..8539 /label=BBa_M1397 misc_feature 7387..8539 /label=BBa_M1396 stem_loop 8555..8574 /label=T7 TE misc_feature 8581..8581 /label=stop polyA_site 8575..8588 /label=polya misc_feature 8548..8588 /label=BBa_B0012 terminator 8548..8588 /label=BBa_B0012 stem_loop 8609..8631 /label=stem_loop misc_feature 8597..8642 /label=BBa_B0011 terminator 8597..8642 /label=BBa_B0011 terminator 8548..8642 /label=BBa_B0014 misc_feature 1529..8642 /label=BBa_M1354 oriT 8651..9359 /label=BBa_M1356 ORIGIN 1 taagtctagt cttgctcttg aaggcggcgg cacccaaaca gcaactccag gacaaacctt 61 caatccggca ccagttgttt ttaacaatac cttccagaac ccatcaagca acgctggaga 121 actagacaca gttcgtctac ttccccttgc cccgaataat actgccgttc cagccacaac 181 tcccgtgcaa gaagcgacag atattcctga tggcactcgt gtaacgatta cagtgggttc 241 cagtagtgct gcttatgatt atgacaatgc cactggttac acatatgtcg ccagctctgg 301 gactgggcca aactttgatg ggacagcaaa caccctaaaa tttgacaata tcagtcctgg 361 agtttccatc aactacacag tcaccattga tctaccggca ggttcccaat tatctgcgga 421 tcgctttagt gcaaacggtg taagtcgtgc cggttattct gtcccagttg ttgcttttgt 481 tgataacaat aattctggcg catttgagtt ctcaacaacg ccttcgtcaa ataatgacaa 541 cgttttcaac atcaagatcg accgcttgta ccttggtcac ctcgctctct taaaagagca 601 acgggtatta aatccgaatg aaacggttga tactgattgg acaactaacc caattgctga 661 tgttgaaccc gggcagttca tcgagtaccg catttcctat gtgaacttct caacaccctc 721 tactagaggt tgatcgggca cgtaagaggt tccaactttc accataatga aataagatca 781 ctaccgggcg tattttttga gttatcgaga ttttcaggag ctaaggaagc taaaatggag 841 aaaaaaatca ctggatatac caccgttgat atatcccaat ggcatcgtaa agaacatttt 901 gaggcatttc agtcagttgc tcaatgtacc tataaccaga ccgttcagct ggatattacg 961 gcctttttaa agaccgtaaa gaaaaataag cacaagtttt atccggcctt tattcacatt 1021 cttgcccgcc tgatgaatgc tcatccggaa tttcgtatgg caatgaaaga cggtgagctg 1081 gtgatatggg atagtgttca cccttgttac accgttttcc atgagcaaac tgaaacgttt 1141 tcatcgctct ggagtgaata ccacgacgat ttccggcagt ttctacacat atattcgcaa 1201 gatgtggcgt gttacggtga aaacctggcc tatttcccta aagggtttat tgagaatatg 1261 tttttcgtct cagccaatcc ctgggtgagt ttcaccagtt ttgatttaaa cgtggccaat 1321 atggacaact tcttcgcccc cgttttcacc atgggcaaat attatacgca aggcgacaag 1381 gtgctgatgc cgctggcgat tcaggttcat catgccgttt gtgatggctt ccatgtcggc 1441 agaatgctta atgaattaca acagtactgc gatgagtggc agggcggggc gtaataattt 1501 gatatcgtgc tcgcttggac tactagagcc tcaactttga ctgaccagct aattttgtac 1561 acgacttagg agttaacgag gaaaacggct actagatgga tttggccgaa tataagggta 1621 tctatgtcat tgcggaacag tttgaaggta aactccggga tgtttctttc gaactcctcg 1681 gtcaagcccg gattctggcg gacaccattg gcgatgaggt aggagccatc ttgatcggca 1741 aggatgttaa gcccttggcg caagagttaa ttgcgcacgg ggcccataaa gtgtacgtgt 1801 acgatgatcc gcagcttgaa cactataata ctaccgctta tgcgaaggtc atttgcgact 1861 tttttcacga agagaagccc aatgtctttt tggtgggcgc gaccaatatc ggtagggacc 1921 tgggtccacg ggtggccaat tccctgaaaa ccggtctgac cgcggattgt acacaattag 1981 gcgttgacga cgacaaaaaa accattgttt ggacccgacc cgcgctgggg ggcaatatca 2041 tggccgaaat tatttgtccc gataatcggc cacagatggg aacagttcgt ccccacgtgt 2101 ttaagaagcc cgaagctgac cccagcgcta ccggtgaagt catcgagaaa aaagccaact 2161 tgtccgacgc cgactttatg accaaatttg tggaattgat caaactcggt ggggaaggtg 2221 tgaaaattga agacgcagat gttattgtgg ccgggggccg cggtatgaac tctgaggaac 2281 ccttcaaaac ggggatcttg aaagaatgcg ccgatgtgct cggtggtgcg gtgggtgcgt 2341 ctcgcgctgc tgtggatgct ggatggattg atgctctgca tcaagtgggt cagacaggga 2401 aaaccgtggg ccccaagatc tacattgcgt gtgcaatcag tggggctatt cagcccttgg 2461 ccgggatgac tggtagcgac tgtatcatcg cgattaataa agacgaagat gcgcccatct 2521 ttaaggtttg tgattatggg attgttggcg acgtttttaa agtcttgccc cttctcactg 2581 aagccattaa gaaacaaaaa gggattgcgt aatactagag tcacactggc tcaccttcgg 2641 gtgggccttt ctgcgtttat atactagaga gagaatataa aaagccagat tattaatccg 2701 gcttttttat tattttacta gagcctcaac tttgactgac cagctaattt tgtacacgac 2761 ttaggagtta acgaggaaaa cggctactag atggagctta ataatgtgat cttagagaaa 2821 gaaggaaaag tggcggtcgt aactattaac cggcccaaag cccttaatgc gctcaatagc 2881 gacacgctaa aggaaatgga ttatgtgatt ggggagatcg aaaacgatag cgaagtgctc 2941 gcggtcattc tcaccggtgc gggggaaaaa tcatttgtgg caggggcgga tatttccgag 3001 atgaaagaaa tgaataccat tgaaggccgt aaatttggta tcttggggaa taaagtattt 3061 cggcgacttg aattgctgga aaaaccagta atcgcagctg tgaatggttt cgcgcttggg 3121 ggaggctgtg aaatcgccat gtcttgtgat atccgcattg cctcgtcaaa cgcccgtttc 3181 ggtcagcccg aagttggctt aggaattacg cccggctttg gcgggacaca acgcctgagc 3241 cgcctcgtcg gtatgggcat ggccaaacaa ttgattttta ctgcccaaaa catcaaggcg 3301 gatgaagctc tgcgcattgg ccttgttaac aaagtagttg agccatctga gctaatgaat 3361 acggcgaaag aaattgcgaa taagatcgta tcaaatgcgc cggtggcagt gaaactcagt 3421 aagcaggcca ttaatagagg tatgcaatgc gatattgata ctgctttagc ctttgaatct 3481 gaagcgttcg gcgaatgttt tagcaccgaa gatcaaaaag atgcaatgac cgcctttatc 3541 gaaaaacgaa aaatcgaagg ttttaaaaat cgctagtact agagtcacac tggctcacct 3601 tcgggtgggc ctttctgcgt ttatatacta gagagagaat ataaaaagcc agattattaa 3661 tccggctttt ttattatttt actagagcct caactttgac tgaccagcta attttgtaca 3721 cgacttagga gttaacgagg aaaacggcta ctagatggac tttaacctca cacgcgaaca 3781 ggaactggtc cgccaaatgg ttcgggcatt cgctgagaac gaagtgaaac cgatcgctgc 3841 cgaaattgac gaaaccgagc ggtttcccat ggaaaacgtt aaaaaaatgg ggcaatatgg 3901 tatgatgggt atcccctttt ctaaagagta cggaggtgct gggggggatg tgttgtcgta 3961 tatcatcgcc gtcgaagaac tcagcaaagt gtgtggcact acgggggtca tcttatcggc 4021 ccataccagc ctatgtgcaa gcttaatcaa tgaacacggt accgaggagc agaaacagaa 4081 atatttggtg cccttagcca aaggtgaaaa aatcggggcg tacgggttaa ccgaacccaa 4141 tgccgggaca gacagtggcg cccaacagac ggtcgccgtc ctagaaggtg atcactacgt 4201 tattaacggg tctaagattt ttatcaccaa tggtggggta gccgatacct ttgtcatttt 4261 tgcgatgacg gaccgcacta aaggcaccaa gggtattagt gcgtttatta tcgaaaaagg 4321 ctttaaaggc ttttccattg gtaaggtcga acaaaaacta ggtatccgtg catcttcgac 4381 cacagaactc gtgtttgaag atatgatcgt gccagtcgaa aacatgattg gcaaagaggg 4441 gaagggcttc ccgattgcta tgaaaaccct cgacgggggc cgtattggga ttgcagccca 4501 ggctttaggc atcgccgaag gtgcctttaa tgaagctcgc gcgtatatga aagaacggaa 4561 acaattcggc cgcagtctcg ataaatttca aggtctggcc tggatgatgg cggatatgga 4621 cgttgccatt gaaagtgccc gctatctcgt ctataaagcg gcctatctca aacaagcagg 4681 actcccctac acagtagacg cggctcgcgc caaattacac gcggcgaatg tcgctatgga 4741 tgtaaccacc aaagccgtgc aattattcgg tggttacggg tataccaagg attatccggt 4801 ggagcggatg atgagagatg caaagatcac cgaaatctat gaagggacgt cggaggtgca 4861 aaaattggtg atctctggta aaatctttcg gtactagagt cacactggct caccttcggg 4921 tgggcctttc tgcgtttata tactagagag agaatataaa aagccagatt attaatccgg 4981 cttttttatt attttactag agcctcaact ttgactgacc agctaatttt gtacacgact 5041 taggagttaa cgaggaaaac ggctactaga tgaaaaaggt atgtgttata ggtgcaggta 5101 ctatgggttc aggaattgct caggcatttg cagctaaagg atttgaagta gtattaagag 5161 atattaaaga tgaatttgtt gatagaggat tagattttat caataaaaat ctttctaaat 5221 tagttaaaaa aggaaagata gaagaagcta ctaaagttga aatcttaact agaatttccg 5281 gaacagttga ccttaatatg gcagctgatt gcgatttagt tatagaagca gctgttgaaa 5341 gaatggatat taaaaagcag atttttgctg acttagacaa tatatgcaag ccagaaacaa 5401 ttcttgcatc aaatacatca tcactttcaa taacagaagt ggcatcagca actaaaagac 5461 ctgataaggt tataggtatg catttcttta atccagctcc tgttatgaag cttgtagagg 5521 taataagagg aatagctaca tcacaagaaa cttttgatgc agttaaagag acatctatag 5581 caataggaaa agatcctgta gaagtagcag aagcaccagg atttgttgta aatagaatat 5641 taataccaat gattaatgaa gcagttggta tattagcaga aggaatagct tcagtagaag 5701 acatagataa agctatgaaa cttggagcta atcacccaat gggaccatta gaattaggtg 5761 attttatagg tcttgatata tgtcttgcta taatggatgt tttatactca gaaactggag 5821 attctaagta tagaccacat acattactta agaagtatgt aagagcagga tggcttggaa 5881 gaaaatcagg aaaaggtttc tacgattatt caaaataata ctagagtcac actggctcac 5941 cttcgggtgg gcctttctgc gtttatatac tagagagaga atataaaaag ccagattatt 6001 aatccggctt ttttattatt ttactagagc ctcaactttg actgaccagc taattttgta 6061 cacgacttag gagttaacga ggaaaacggc tactagagaa tgcgggacgt cgtgatcgtg 6121 agtgctgtgc gaacggccat cggagcttat gggaaaaccc taaaagatgt ccccgccaca 6181 gagctgggtg ctatcgtcat aaaagaggcc gttcggcggg ccaacattaa ccccaacgag 6241 atcaatgaag tcatttttgg taatgtctta caggcaggac ttggccagaa tccggcccgt 6301 caagcggcag tcaaggcggg cctgcccttg gaaacgcccg cctttaccat caataaagta 6361 tgtggaagtg gattgagatc tattagtttg gcggcccaga ttatcaaagc aggagatgcc 6421 gatacgatcg ttgtgggcgg catggaaaat atgtctcgct cgccctacct tattaacaac 6481 cagcgctggg gtcaacgcat gggcgacagc gaacttgtcg acgaaatgat taaagatggt 6541 ctctgggatg cctttaacgg ttatcacatg ggggttacag cggaaaatat cgctgaacaa 6601 tggaacatta cccgcgagga acaagatgaa ttttccttaa tgtctcaaca aaaagcagaa 6661 aaagccatta agaatgggga gtttaaagac gaaatcgtcc ccgtgctcat taaaactaaa 6721 aaaggtgaaa tcgtctttga tcaagatgga ttcccccggt ttggcaacac catcgaagct 6781 ttgcggaagc tcaaaccgat ttttaaagaa aacgggaccg tcaccgctgg caacgcgtct 6841 ggcttaaatg atggcgccgc ggccttggta attatgtctg ccgataaggc aaacgcgctg 6901 ggtattaaac ccctcgccaa aattaccagt tacggctctt atggtgtcga tccctcgatc 6961 atgggctacg gtgcatttta cgctacaaaa gctgccctcg acaagattaa cttgaagccg 7021 gaagatctcg acctcattga ggcgaatgaa gcatatgcaa gtcagtccat cgctgtcacg 7081 cgggacttga atctggacat gtctaaagtc aatgtgaacg gcggcgccat cgcgttaggt 7141 catcccatcg gggcttctgg cgcgcgtatt ctcgtcacgc tcctttacgc tatgcaaaag 7201 cgcgattcga aaaaagggtt agcgacgctc tgcatcgggg gtggacaggg aaccgctctc 7261 gtcgtcgaac gggactacta gagtcacact ggctcacctt cgggtgggcc tttctgcgtt 7321 tatatactag agagagaata taaaaagcca gattattaat ccggcttttt tattatttta 7381 ctagagcctc aactttgact gaccagctaa ttttgtacac gacttaggag ttaacgagga 7441 aaacggctac tagatgagcc aaacggttcg gggcgtgatt gcacgccaaa aaggtgaacc 7501 tgttgaatta gtcaatattg tcgtccccga tcccggccct ggcgaagccg tcgtcgatgt 7561 gactgcctgc ggggtgtgtc acacagactt aacttatcgc gaagggggta tcaatgacga 7621 gtatcctttc ttgctgggcc acgaggctgc cgggattatt gaggcggtcg gtcccggcgt 7681 gacagctgtt gaacccggtg atttcgtcat cttaaactgg cgtgcggtct gtgggcaatg 7741 ccgtgcttgt aaacgcggcc ggccacgtta ctgttttgat accttcaatg cggaacaaaa 7801 aatgacgctc acggatggaa ccgaactcac tgccgcccta ggcattgggg cctttgcgga 7861 taagaccctc gtgcattccg gccaatgtac caaggttgat cccgcggcag atccggctgt 7921 cgcaggtttg ctcggctgtg gtgtcatggc gggtttgggc gctgcaatta acacaggggg 7981 tgtgacacgg gatgatactg ttgctgtaat tggctgtggt ggagtgggtg atgctgctat 8041 tgcaggggct gcacttgttg gtgccaaacg cattattgcc gtggataccg atgatacgaa 8101 attggattgg gctagaacgt ttggcgcaac ccacactgtc aacgcacgcg aagtagatgt 8161 cgtacaagcc attggaggac ttactgatgg ttttggagct gacgttgtca ttgatgcggt 8221 aggccgaccg gaaacgtatc agcaagcctt ttacgcgagg gacctagccg gtacggtagt 8281 acttgttggg gtcccgactc ccgatatgcg cctcgacatg cctctcgtcg attttttcag 8341 tcatggtggc gcgctcaaaa gcagttggta cggtgattgc cttcctgaga gcgattttcc 8401 cactcttatt gatttgtact tgcagggccg cttacccttg cagcgctttg tgtcggaacg 8461 catcggactt gaagatgtcg aggaagcctt tcataagatg cacggcggta aggtcctccg 8521 gtctgtggtt atgctctgat actagagtca cactggctca ccttcgggtg ggcctttctg 8581 cgtttatata ctagagagag aatataaaaa gccagattat taatccggct tttttattat 8641 tttactagag caccttgccc gatggtcgtg tggaaacccg cccggcccca gcggaacgct 8701 atacccatgt gcgttggact ttccaaaatg ctgtccctgc aagggctagc gtgaatggcc 8761 agtaccaagt ccgcgttcaa taaacgtcaa acattcactg gtctttactt ttttggaggc 8821 ctttgtcggc cagagtcttt ttaatgccta attttcttga ccattcacga ctaatcctca 8881 agcgatcgcc actggcgatc gccctagtta gcgttggctt gaatttaggt atgcctttgg 8941 ttttggccca gtccctggag acagtagaga atatcaccct cttcaaccgg gccgaagcca 9001 gttaccaaat tgaggagaat ggcccagccg caaatatcac cagtaaccaa gtgatccttg 9061 gctctggtaa tccgccaccg ccaccaccac ctattgatcc ctttggccgt atcctcggct 9121 gtgccggaga gcccttggac aattacagcg gttttttggt gggactctat gaagctgatg 9181 gccccttaca aactgaaatt ggcgcgttgc ttaccctcga tccgctcact gggtcagtgc 9241 cgcttcctcc tggggtggtc aatgtcaacg aagagaacgc taatcccttt gaccttggca 9301 tcaccgagac cctaggcgac ccggattttc ggggtcaatt taattttttc ctacaacct //