LOCUS       BBa_M1528               3609 bp    DNA     linear       01-Jun-2024
DEFINITION  RBS + HlyB + RBS + HlyD
ACCESSION   BBa_M1528
VERSION     BBa_M1528.1  
FEATURES             Location/Qualifiers
     RBS             1..14
                     /label=BBa_B0035
     misc_feature    21..23
                     /label=Start Codon
     misc_feature    2142..2144
                     /label=Stop Codon
     CDS             21..2141
                     /label=HlyB
     CDS             21..2144
                     /label=BBa_M1003
     RBS             2153..2166
                     /label=BBa_B0035
     misc_feature    2173..2175
                     /label=Start Codon
     CDS             2173..3606
                     /label=HlyD
     misc_feature    3607..3609
                     /label=Stop Codon
     CDS             2173..3609
                     /label=BBa_M1004
ORIGIN
        1 attaaagagg agaatactag atggattcct gtcacaagat tgattacggc ctgtatgcac
       61 tggaaatcct ggcacaatat cataacgtct ccgtcaaccc ggaggaaatt aaacatcgct
      121 tcgatactga cggcactggc ctgggtctga ccagctggct gctggcagcg aagtccctgg
      181 agctgaaggt aaagcaagtg aaaaagacga ttgatcgtct gaacttcatc agcctgccgg
      241 cactggtttg gcgtgaagat ggccgtcact tcatcctgac gaaagtgtcc aaagaagcga
      301 accgctacct gatctttgat ctggagcagc gcaacccgcg cgtactggaa cagagcgagt
      361 tcgaggctct gtatcagggc cacatcattc tgattgcatc tcgttcttct gttgcaggta
      421 aactggcgaa attcgacttc acctggttca ttccggctat tatcaagtac cgtcgtattt
      481 tcatcgaaac cctggttgta tctgttttcc tgcaactgtt cgctctgatt actccgctgt
      541 tctttcaggt tgtcatggac aaagttctgg ttcaccgtgg cttttccacc ctgaacgtca
      601 ttactgttgc gctgagcgtt gttgttgtgt tcgaaatcat tctgtccggt ctgcgtacct
      661 acatcttcgc gcactctacc tcccgcatcg acgttgaact gggtgcgaag ctgttccgtc
      721 acctgctggc actgcctatc agctacttcg aatctcgtcg tgtgggcgat actgtggccc
      781 gtgttcgtga actggatcag atccgcaact ttctgaccgg ccaggccctg accagcgttc
      841 tggatctgct gttcagcttt atcttcttcg cagtaatgtg gtactacagc ccgaagctga
      901 ctctggtgat tctgttctct ctgccgtgtt acgcagcttg gtccgtcttc atctctccaa
      961 tcctgcgtcg ccgtctggac gacaaattta gccgtaatgc ggataatcag tccttcctgg
     1021 tcgaatctgt gactgctatc aacactatca aagccatggc ggtttctcca cagatgacta
     1081 acatctggga taagcagctg gcaggttacg ttgctgcggg cttcaaagtg accgttctgg
     1141 caaccattgg tcaacagggc attcagctga ttcagaaaac cgtgatgatc attaacctgt
     1201 ggctgggtgc gcacctggtc atcagcggtg acctgtccat tggccagctg atcgctttca
     1261 acatgctggc gggtcaaatt gttgccccag taatccgtct ggcccagatc tggcaggact
     1321 tccagcaggt cggtatttct gtgacccgcc tgggtgacgt cctgaactct ccgaccgaaa
     1381 gctaccacgg taaactggct ctgccggaga tcaacggcga tattactttc cgtaacatcc
     1441 gttttcgtta caaaccggat tccccggtaa tcctggataa catcaatctg tctatcaaac
     1501 agggcgaagt catcggcatc gtgggccgta gcggcagcgg caagtccact ctgactaaac
     1561 tgattcagcg cttttacatt ccagaaaacg gtcaggttct gattgacggt cacgacctgg
     1621 ctctggcgga cccgaactgg ctgcgtcgtc aggtgggcgt agtgctgcaa gataacgtgc
     1681 tgctgaaccg tagcatcatc gataacatca gcctggctaa cccgggcatg tccgtggaaa
     1741 aagttatcta tgctgcgaaa ctggcaggcg cgcacgattt catctctgaa ctgcgtgaag
     1801 gctacaacac catcgtaggc gaacagggcg ctggcctgtc cggcggtcaa cgtcagcgta
     1861 tcgctattgc acgtgcactg gtgaacaacc cgaaaatcct gatcttcgac gaggccactt
     1921 ctgctctgga ttacgaatct gaacatatta tcatgcgtaa tatgcacaag atctgcaaag
     1981 gtcgcaccgt aatcatcatc gcccaccgtc tgagcaccgt aaagaacgcc gaccgcatca
     2041 tcgtgatgga aaaaggtaag atcgttgaac agggtaaaca taaagagctg ctgtccgaac
     2101 cggaatctct gtattcttat ctgtaccagc tgcaatctga ttaatactag agattaaaga
     2161 ggagaatact agatgaaaac ttggctgatg ggcttctctg agttcctgct gcgttataag
     2221 ctggtatgga gcgaaacttg gaaaatccgt aagcaactgg ataccccggt acgtgaaaaa
     2281 gacgaaaacg aatttctgcc agcacatctg gaactgattg agacgccggt ttctcgccgt
     2341 ccacgtctgg ttgcttactt cattatgggc ttcctggtta ttgctgtaat cctgtctgtt
     2401 ctgggtcagg tggaaatcgt tgcgaccgcg aatggtaaac tgaccctgtc cggtcgttct
     2461 aaagaaatca aaccgatcga aaactccatc gttaaagaga ttatcgttaa agaaggtgaa
     2521 tccgtacgca aaggcgatgt tctgctgaaa ctgaccgcac tgggcgcaga agcggacact
     2581 ctgaaaactc agtcctctct gctgcaaacc cgtctggaac agacccgtta ccaaatcctg
     2641 agccgttcta ttgaactgaa caaactgccg gaactgaaac tgccggatga accatacttc
     2701 cagaacgtaa gcgaggagga agttctgcgc ctgacttccc tgatcaaaga gcagttctct
     2761 acttggcaga accagaaata ccaaaaggaa ctgaacctgg acaaaaagcg tgcggagcgc
     2821 ctgactatcc tggcccgcat caaccgttac gagaacctgt ctcgtgttga aaaatctcgc
     2881 ctggacgact tccgtagcct gctgcacaaa caggccatcg caaaacacgc tgtgctggaa
     2941 caggaaaata aatacgtgga agctgccaac gaactgcgtg tatataaaag ccagctggag
     3001 cagatcgaga gcgaaatcct gtctgcgaag gaggagtacc agctggtgac gcagctgttc
     3061 aaaaatgaaa tcctggacaa actgcgtcaa accacggact ctatcgaact gctgaccctg
     3121 gaactggaga aaaacgagga gcgccagcag gccagcgtca tccgcgctcc ggtatctggt
     3181 aaagttcagc aactgaaagt tcacaccgaa ggtggcgttg ttaccaccgc agaaaccctg
     3241 atggttattg tgccggaaga tgacaccctg gaagttaccg ctctggtgca gaacaaagat
     3301 atcggtttca tcaacgtcgg tcagaacgcg atcatcaaag tagaggcttt cccgtacact
     3361 cgttacggct acctggtggg caaggtcaaa aacatcaacc tggacgctat cgaagatcag
     3421 aaactgggcc tggtgttcaa cgtgatcgtt agcgtagaag agaacgatct gtctaccggt
     3481 aacaaacaca ttccactgtc ctccggcatg gcggttactg ctgaaatcaa aaccggtatg
     3541 cgtagcgtga tctcctatct gctgtctccg ctggaagaaa gcgtcaccga atctctgcac
     3601 gaacgctaa
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