LOCUS BBa_M1540 5630 bp DNA linear 17-May-2024 DEFINITION YL2 - antifreeze protein - II ACCESSION BBa_M1540 VERSION BBa_M1540.1 FEATURES Location/Qualifiers RBS 209..220 /label=BBa_B0034 promoter 1..200 /label=BBa_R0010 promoter 1..220 /label=BBa_K208010 misc_feature 227..229 /label=start misc_feature 230..253 /label=tag misc_feature 227..253 /label=BBa_M1534 misc_feature 260..280 /label=start misc_feature 730..740 /label=stop CDS 260..742 /label=BBa_M1538 misc_feature 751..936 /label=HlyA misc_feature 751..753 /label=Start misc_feature 751..936 /label=BBa_K208006 RBS 945..958 /label=BBa_B0035 misc_feature 965..967 /label=Start Codon misc_feature 3086..3088 /label=Stop Codon CDS 965..3085 /label=HlyB CDS 965..3088 /label=BBa_M1003 RBS 3097..3110 /label=BBa_B0035 misc_feature 3117..3119 /label=Start Codon CDS 3117..4550 /label=HlyD misc_feature 4551..4553 /label=Stop Codon CDS 3117..4553 /label=BBa_M1004 misc_feature 945..4553 /label=BBa_M1528 misc_feature 4566..4569 /label=conserved RBS 4562..4573 /label=BBa_B0034 misc_feature 4580..4582 /label=Start CDS 4580..5299 /label=GFP Coding Region CDS 4580..5299 /label=BBa_K208000 misc_feature 5308..5493 /label=HlyA misc_feature 5308..5310 /label=Start misc_feature 5308..5493 /label=BBa_K208006 stem_loop 5513..5556 /label=stem_loop misc_feature 5502..5581 /label=BBa_B0010 terminator 5502..5581 /label=BBa_B0010 stem_loop 5597..5616 /label=T7 TE misc_feature 5623..5623 /label=stop polyA_site 5617..5630 /label=polya misc_feature 5590..5630 /label=BBa_B0012 terminator 5590..5630 /label=BBa_B0012 terminator 5502..5630 /label=BBa_B0015 ORIGIN 1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca 61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc 121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga 181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatgc atcatcacca 241 tcatcaccat cactactaga tggctttcaa aacgtgcgga ttcagcaaga aatggttggt 301 tattgccgtt atagtcatgt gcctttgtac tgaatgctat tgtcactgta cgggtggagc 361 cgattgcacc tcctgtactg atgcttgcac gggttgtggt aattgcccaa acgcgcatac 421 ctgtactgac agcaagaatg tttgtaaggc ggcaacctgt acagatagtg agaattgtgt 481 gaaagcccat acatgtaccg gcagtacgaa atgtaacacg gcgagaacat gtactaactc 541 taaggactgc tttgaagcga aaacgtgtac cgggagtacg cattgtcatc gtgcgaccac 601 ttgcactaat tcaaaagatt gtttcgaagc caccacctgt acagggagtt cgaattgtta 661 tacagcgacc acgtgtacag acagtaccaa ttgctataaa gctaccgcgt gtaccaattc 721 gaccgggtgt ccaggacatt agtactagag ttagcctatg gaagtcaggg tgatcttaat 781 ccattaatta atgaaatcag caaaatcatt tcagcagcag gtagcttcga tgttaaagag 841 gaaagaaccg cagcttcttt attgcagttg tccggtaatg ccagtgattt ttcatatgga 901 cggaactcaa taaccctgac cacatcagca taataatact agagattaaa gaggagaata 961 ctagatggat tcctgtcaca agattgatta cggcctgtat gcactggaaa tcctggcaca 1021 atatcataac gtctccgtca acccggagga aattaaacat cgcttcgata ctgacggcac 1081 tggcctgggt ctgaccagct ggctgctggc agcgaagtcc ctggagctga aggtaaagca 1141 agtgaaaaag acgattgatc gtctgaactt catcagcctg ccggcactgg tttggcgtga 1201 agatggccgt cacttcatcc tgacgaaagt gtccaaagaa gcgaaccgct acctgatctt 1261 tgatctggag cagcgcaacc cgcgcgtact ggaacagagc gagttcgagg ctctgtatca 1321 gggccacatc attctgattg catctcgttc ttctgttgca ggtaaactgg cgaaattcga 1381 cttcacctgg ttcattccgg ctattatcaa gtaccgtcgt attttcatcg aaaccctggt 1441 tgtatctgtt ttcctgcaac tgttcgctct gattactccg ctgttctttc aggttgtcat 1501 ggacaaagtt ctggttcacc gtggcttttc caccctgaac gtcattactg ttgcgctgag 1561 cgttgttgtt gtgttcgaaa tcattctgtc cggtctgcgt acctacatct tcgcgcactc 1621 tacctcccgc atcgacgttg aactgggtgc gaagctgttc cgtcacctgc tggcactgcc 1681 tatcagctac ttcgaatctc gtcgtgtggg cgatactgtg gcccgtgttc gtgaactgga 1741 tcagatccgc aactttctga ccggccaggc cctgaccagc gttctggatc tgctgttcag 1801 ctttatcttc ttcgcagtaa tgtggtacta cagcccgaag ctgactctgg tgattctgtt 1861 ctctctgccg tgttacgcag cttggtccgt cttcatctct ccaatcctgc gtcgccgtct 1921 ggacgacaaa tttagccgta atgcggataa tcagtccttc ctggtcgaat ctgtgactgc 1981 tatcaacact atcaaagcca tggcggtttc tccacagatg actaacatct gggataagca 2041 gctggcaggt tacgttgctg cgggcttcaa agtgaccgtt ctggcaacca ttggtcaaca 2101 gggcattcag ctgattcaga aaaccgtgat gatcattaac ctgtggctgg gtgcgcacct 2161 ggtcatcagc ggtgacctgt ccattggcca gctgatcgct ttcaacatgc tggcgggtca 2221 aattgttgcc ccagtaatcc gtctggccca gatctggcag gacttccagc aggtcggtat 2281 ttctgtgacc cgcctgggtg acgtcctgaa ctctccgacc gaaagctacc acggtaaact 2341 ggctctgccg gagatcaacg gcgatattac tttccgtaac atccgttttc gttacaaacc 2401 ggattccccg gtaatcctgg ataacatcaa tctgtctatc aaacagggcg aagtcatcgg 2461 catcgtgggc cgtagcggca gcggcaagtc cactctgact aaactgattc agcgctttta 2521 cattccagaa aacggtcagg ttctgattga cggtcacgac ctggctctgg cggacccgaa 2581 ctggctgcgt cgtcaggtgg gcgtagtgct gcaagataac gtgctgctga accgtagcat 2641 catcgataac atcagcctgg ctaacccggg catgtccgtg gaaaaagtta tctatgctgc 2701 gaaactggca ggcgcgcacg atttcatctc tgaactgcgt gaaggctaca acaccatcgt 2761 aggcgaacag ggcgctggcc tgtccggcgg tcaacgtcag cgtatcgcta ttgcacgtgc 2821 actggtgaac aacccgaaaa tcctgatctt cgacgaggcc acttctgctc tggattacga 2881 atctgaacat attatcatgc gtaatatgca caagatctgc aaaggtcgca ccgtaatcat 2941 catcgcccac cgtctgagca ccgtaaagaa cgccgaccgc atcatcgtga tggaaaaagg 3001 taagatcgtt gaacagggta aacataaaga gctgctgtcc gaaccggaat ctctgtattc 3061 ttatctgtac cagctgcaat ctgattaata ctagagatta aagaggagaa tactagatga 3121 aaacttggct gatgggcttc tctgagttcc tgctgcgtta taagctggta tggagcgaaa 3181 cttggaaaat ccgtaagcaa ctggataccc cggtacgtga aaaagacgaa aacgaatttc 3241 tgccagcaca tctggaactg attgagacgc cggtttctcg ccgtccacgt ctggttgctt 3301 acttcattat gggcttcctg gttattgctg taatcctgtc tgttctgggt caggtggaaa 3361 tcgttgcgac cgcgaatggt aaactgaccc tgtccggtcg ttctaaagaa atcaaaccga 3421 tcgaaaactc catcgttaaa gagattatcg ttaaagaagg tgaatccgta cgcaaaggcg 3481 atgttctgct gaaactgacc gcactgggcg cagaagcgga cactctgaaa actcagtcct 3541 ctctgctgca aacccgtctg gaacagaccc gttaccaaat cctgagccgt tctattgaac 3601 tgaacaaact gccggaactg aaactgccgg atgaaccata cttccagaac gtaagcgagg 3661 aggaagttct gcgcctgact tccctgatca aagagcagtt ctctacttgg cagaaccaga 3721 aataccaaaa ggaactgaac ctggacaaaa agcgtgcgga gcgcctgact atcctggccc 3781 gcatcaaccg ttacgagaac ctgtctcgtg ttgaaaaatc tcgcctggac gacttccgta 3841 gcctgctgca caaacaggcc atcgcaaaac acgctgtgct ggaacaggaa aataaatacg 3901 tggaagctgc caacgaactg cgtgtatata aaagccagct ggagcagatc gagagcgaaa 3961 tcctgtctgc gaaggaggag taccagctgg tgacgcagct gttcaaaaat gaaatcctgg 4021 acaaactgcg tcaaaccacg gactctatcg aactgctgac cctggaactg gagaaaaacg 4081 aggagcgcca gcaggccagc gtcatccgcg ctccggtatc tggtaaagtt cagcaactga 4141 aagttcacac cgaaggtggc gttgttacca ccgcagaaac cctgatggtt attgtgccgg 4201 aagatgacac cctggaagtt accgctctgg tgcagaacaa agatatcggt ttcatcaacg 4261 tcggtcagaa cgcgatcatc aaagtagagg ctttcccgta cactcgttac ggctacctgg 4321 tgggcaaggt caaaaacatc aacctggacg ctatcgaaga tcagaaactg ggcctggtgt 4381 tcaacgtgat cgttagcgta gaagagaacg atctgtctac cggtaacaaa cacattccac 4441 tgtcctccgg catggcggtt actgctgaaa tcaaaaccgg tatgcgtagc gtgatctcct 4501 atctgctgtc tccgctggaa gaaagcgtca ccgaatctct gcacgaacgc taatactaga 4561 gaaagaggag aaatactaga tggctagcaa aggagaagaa cttttcactg gagttgtccc 4621 aattcttgtt gaattagatg gtgatgttaa tgggcacaaa ttttctgtca gtggagaggg 4681 tgaaggtgat gctacatacg gaaagcttac ccttaaattt atttgcacta ctggaaaact 4741 acctgttcca tggccaacac ttgtcactac tttctcttat ggtgttcaat gcttttcccg 4801 ttatccggat catatgaaac ggcatgactt tttcaagagt gccatgcccg aaggttatgt 4861 acaggaacgc actatatctt tcaaagatga cgggaactac aagacgcgtg ctgaagtcaa 4921 gtttgaaggt gatacccttg ttaatcgtat cgagttaaaa ggtattgatt ttaaagaaga 4981 tggaaacatt ctcggacaca aactcgagta caactataac tcacacaatg tatacatcac 5041 ggcagacaaa caaaagaatg gaatcaaagc taacttcaaa attcgccaca acattgaaga 5101 tggatccgtt caactagcag accattatca acaaaatact ccaattggcg atggccctgt 5161 ccttttacca gacaaccatt acctgtcgac acaatctgcc ctttcgaaag atcccaacga 5221 aaagcgtgac cacatggtcc ttcttgagtt tgtaactgct gctgggatta cacatggcat 5281 ggatgagctc tacaaataat actagagtta gcctatggaa gtcagggtga tcttaatcca 5341 ttaattaatg aaatcagcaa aatcatttca gcagcaggta gcttcgatgt taaagaggaa 5401 agaaccgcag cttctttatt gcagttgtcc ggtaatgcca gtgatttttc atatggacgg 5461 aactcaataa ccctgaccac atcagcataa taatactaga gccaggcatc aaataaaacg 5521 aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct 5581 ctactagagt cacactggct caccttcggg tgggcctttc tgcgtttata //