LOCUS BBa_M1541 5486 bp DNA linear 17-May-2024 DEFINITION Thermal Hysteresis (Antifreeze) Protein (variant YL3) with 8-His tag with secretion tag and GFP ACCESSION BBa_M1541 VERSION BBa_M1541.1 FEATURES Location/Qualifiers RBS 209..220 /label=BBa_B0034 promoter 1..200 /label=BBa_R0010 promoter 1..220 /label=BBa_K208010 misc_feature 227..229 /label=Translation Start CDS 227..598 /label=Coding Sequence CDS 227..598 /label=BBa_M1521 misc_feature 607..792 /label=HlyA misc_feature 607..609 /label=Start misc_feature 607..792 /label=BBa_K208006 RBS 801..814 /label=BBa_B0035 misc_feature 821..823 /label=Start Codon misc_feature 2942..2944 /label=Stop Codon CDS 821..2941 /label=HlyB CDS 821..2944 /label=BBa_M1003 RBS 2953..2966 /label=BBa_B0035 misc_feature 2973..2975 /label=Start Codon CDS 2973..4406 /label=HlyD misc_feature 4407..4409 /label=Stop Codon CDS 2973..4409 /label=BBa_M1004 misc_feature 801..4409 /label=BBa_M1528 misc_feature 4422..4425 /label=conserved RBS 4418..4429 /label=BBa_B0034 misc_feature 4436..4438 /label=Start CDS 4436..5155 /label=GFP Coding Region CDS 4436..5155 /label=BBa_K208000 misc_feature 5164..5349 /label=HlyA misc_feature 5164..5166 /label=Start misc_feature 5164..5349 /label=BBa_K208006 stem_loop 5369..5412 /label=stem_loop misc_feature 5358..5437 /label=BBa_B0010 terminator 5358..5437 /label=BBa_B0010 stem_loop 5453..5472 /label=T7 TE misc_feature 5479..5479 /label=stop polyA_site 5473..5486 /label=polya misc_feature 5446..5486 /label=BBa_B0012 terminator 5446..5486 /label=BBa_B0012 terminator 5358..5486 /label=BBa_B0015 ORIGIN 1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca 61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc 121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga 181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatgg cctttaaaac 241 atgcggattt agtaaaaaat ggttaatcat agccgttatt gtgatgtgct tatgtacaga 301 atgctattgc cagtgcacag gaggtgctga ttgtaccagt tgcaccgctg cctgcacggg 361 ctgcggctct tgtcccaatg ctcatacgtg tacagattca aaaaattgtg ttcgtgcaga 421 aacctgtaca gattccgaaa attgcgtgaa agcccatact tgtacgggtt cccgcaattg 481 caataccgca atgacgtgta caaactcgaa agactgcttt gaagcaaaaa cctgtaccga 541 ttccaccaat tgttataagg caaccgcgtg tactaattct accgggtgtc caggacatta 601 ctagagttag cctatggaag tcagggtgat cttaatccat taattaatga aatcagcaaa 661 atcatttcag cagcaggtag cttcgatgtt aaagaggaaa gaaccgcagc ttctttattg 721 cagttgtccg gtaatgccag tgatttttca tatggacgga actcaataac cctgaccaca 781 tcagcataat aatactagag attaaagagg agaatactag atggattcct gtcacaagat 841 tgattacggc ctgtatgcac tggaaatcct ggcacaatat cataacgtct ccgtcaaccc 901 ggaggaaatt aaacatcgct tcgatactga cggcactggc ctgggtctga ccagctggct 961 gctggcagcg aagtccctgg agctgaaggt aaagcaagtg aaaaagacga ttgatcgtct 1021 gaacttcatc agcctgccgg cactggtttg gcgtgaagat ggccgtcact tcatcctgac 1081 gaaagtgtcc aaagaagcga accgctacct gatctttgat ctggagcagc gcaacccgcg 1141 cgtactggaa cagagcgagt tcgaggctct gtatcagggc cacatcattc tgattgcatc 1201 tcgttcttct gttgcaggta aactggcgaa attcgacttc acctggttca ttccggctat 1261 tatcaagtac cgtcgtattt tcatcgaaac cctggttgta tctgttttcc tgcaactgtt 1321 cgctctgatt actccgctgt tctttcaggt tgtcatggac aaagttctgg ttcaccgtgg 1381 cttttccacc ctgaacgtca ttactgttgc gctgagcgtt gttgttgtgt tcgaaatcat 1441 tctgtccggt ctgcgtacct acatcttcgc gcactctacc tcccgcatcg acgttgaact 1501 gggtgcgaag ctgttccgtc acctgctggc actgcctatc agctacttcg aatctcgtcg 1561 tgtgggcgat actgtggccc gtgttcgtga actggatcag atccgcaact ttctgaccgg 1621 ccaggccctg accagcgttc tggatctgct gttcagcttt atcttcttcg cagtaatgtg 1681 gtactacagc ccgaagctga ctctggtgat tctgttctct ctgccgtgtt acgcagcttg 1741 gtccgtcttc atctctccaa tcctgcgtcg ccgtctggac gacaaattta gccgtaatgc 1801 ggataatcag tccttcctgg tcgaatctgt gactgctatc aacactatca aagccatggc 1861 ggtttctcca cagatgacta acatctggga taagcagctg gcaggttacg ttgctgcggg 1921 cttcaaagtg accgttctgg caaccattgg tcaacagggc attcagctga ttcagaaaac 1981 cgtgatgatc attaacctgt ggctgggtgc gcacctggtc atcagcggtg acctgtccat 2041 tggccagctg atcgctttca acatgctggc gggtcaaatt gttgccccag taatccgtct 2101 ggcccagatc tggcaggact tccagcaggt cggtatttct gtgacccgcc tgggtgacgt 2161 cctgaactct ccgaccgaaa gctaccacgg taaactggct ctgccggaga tcaacggcga 2221 tattactttc cgtaacatcc gttttcgtta caaaccggat tccccggtaa tcctggataa 2281 catcaatctg tctatcaaac agggcgaagt catcggcatc gtgggccgta gcggcagcgg 2341 caagtccact ctgactaaac tgattcagcg cttttacatt ccagaaaacg gtcaggttct 2401 gattgacggt cacgacctgg ctctggcgga cccgaactgg ctgcgtcgtc aggtgggcgt 2461 agtgctgcaa gataacgtgc tgctgaaccg tagcatcatc gataacatca gcctggctaa 2521 cccgggcatg tccgtggaaa aagttatcta tgctgcgaaa ctggcaggcg cgcacgattt 2581 catctctgaa ctgcgtgaag gctacaacac catcgtaggc gaacagggcg ctggcctgtc 2641 cggcggtcaa cgtcagcgta tcgctattgc acgtgcactg gtgaacaacc cgaaaatcct 2701 gatcttcgac gaggccactt ctgctctgga ttacgaatct gaacatatta tcatgcgtaa 2761 tatgcacaag atctgcaaag gtcgcaccgt aatcatcatc gcccaccgtc tgagcaccgt 2821 aaagaacgcc gaccgcatca tcgtgatgga aaaaggtaag atcgttgaac agggtaaaca 2881 taaagagctg ctgtccgaac cggaatctct gtattcttat ctgtaccagc tgcaatctga 2941 ttaatactag agattaaaga ggagaatact agatgaaaac ttggctgatg ggcttctctg 3001 agttcctgct gcgttataag ctggtatgga gcgaaacttg gaaaatccgt aagcaactgg 3061 ataccccggt acgtgaaaaa gacgaaaacg aatttctgcc agcacatctg gaactgattg 3121 agacgccggt ttctcgccgt ccacgtctgg ttgcttactt cattatgggc ttcctggtta 3181 ttgctgtaat cctgtctgtt ctgggtcagg tggaaatcgt tgcgaccgcg aatggtaaac 3241 tgaccctgtc cggtcgttct aaagaaatca aaccgatcga aaactccatc gttaaagaga 3301 ttatcgttaa agaaggtgaa tccgtacgca aaggcgatgt tctgctgaaa ctgaccgcac 3361 tgggcgcaga agcggacact ctgaaaactc agtcctctct gctgcaaacc cgtctggaac 3421 agacccgtta ccaaatcctg agccgttcta ttgaactgaa caaactgccg gaactgaaac 3481 tgccggatga accatacttc cagaacgtaa gcgaggagga agttctgcgc ctgacttccc 3541 tgatcaaaga gcagttctct acttggcaga accagaaata ccaaaaggaa ctgaacctgg 3601 acaaaaagcg tgcggagcgc ctgactatcc tggcccgcat caaccgttac gagaacctgt 3661 ctcgtgttga aaaatctcgc ctggacgact tccgtagcct gctgcacaaa caggccatcg 3721 caaaacacgc tgtgctggaa caggaaaata aatacgtgga agctgccaac gaactgcgtg 3781 tatataaaag ccagctggag cagatcgaga gcgaaatcct gtctgcgaag gaggagtacc 3841 agctggtgac gcagctgttc aaaaatgaaa tcctggacaa actgcgtcaa accacggact 3901 ctatcgaact gctgaccctg gaactggaga aaaacgagga gcgccagcag gccagcgtca 3961 tccgcgctcc ggtatctggt aaagttcagc aactgaaagt tcacaccgaa ggtggcgttg 4021 ttaccaccgc agaaaccctg atggttattg tgccggaaga tgacaccctg gaagttaccg 4081 ctctggtgca gaacaaagat atcggtttca tcaacgtcgg tcagaacgcg atcatcaaag 4141 tagaggcttt cccgtacact cgttacggct acctggtggg caaggtcaaa aacatcaacc 4201 tggacgctat cgaagatcag aaactgggcc tggtgttcaa cgtgatcgtt agcgtagaag 4261 agaacgatct gtctaccggt aacaaacaca ttccactgtc ctccggcatg gcggttactg 4321 ctgaaatcaa aaccggtatg cgtagcgtga tctcctatct gctgtctccg ctggaagaaa 4381 gcgtcaccga atctctgcac gaacgctaat actagagaaa gaggagaaat actagatggc 4441 tagcaaagga gaagaacttt tcactggagt tgtcccaatt cttgttgaat tagatggtga 4501 tgttaatggg cacaaatttt ctgtcagtgg agagggtgaa ggtgatgcta catacggaaa 4561 gcttaccctt aaatttattt gcactactgg aaaactacct gttccatggc caacacttgt 4621 cactactttc tcttatggtg ttcaatgctt ttcccgttat ccggatcata tgaaacggca 4681 tgactttttc aagagtgcca tgcccgaagg ttatgtacag gaacgcacta tatctttcaa 4741 agatgacggg aactacaaga cgcgtgctga agtcaagttt gaaggtgata cccttgttaa 4801 tcgtatcgag ttaaaaggta ttgattttaa agaagatgga aacattctcg gacacaaact 4861 cgagtacaac tataactcac acaatgtata catcacggca gacaaacaaa agaatggaat 4921 caaagctaac ttcaaaattc gccacaacat tgaagatgga tccgttcaac tagcagacca 4981 ttatcaacaa aatactccaa ttggcgatgg ccctgtcctt ttaccagaca accattacct 5041 gtcgacacaa tctgcccttt cgaaagatcc caacgaaaag cgtgaccaca tggtccttct 5101 tgagtttgta actgctgctg ggattacaca tggcatggat gagctctaca aataatacta 5161 gagttagcct atggaagtca gggtgatctt aatccattaa ttaatgaaat cagcaaaatc 5221 atttcagcag caggtagctt cgatgttaaa gaggaaagaa ccgcagcttc tttattgcag 5281 ttgtccggta atgccagtga tttttcatat ggacggaact caataaccct gaccacatca 5341 gcataataat actagagcca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc 5401 ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac tagagtcaca ctggctcacc 5461 ttcgggtggg cctttctgcg tttata //