BBa_B0012
1
BBa_B0012
TE from coliphageT7
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
Released HQ 2013
Transcription terminator for the <i>E.coli</i> RNA polymerase.
false
false
_1_
0
24
7
In stock
false
<P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
false
Reshma Shetty
annotation7020
1
BBa_B0012
range7020
1
1
41
annotation1686
1
T7 TE
range1686
1
8
27
annotation1690
1
polya
range1690
1
28
41
annotation1687
1
stop
range1687
1
34
34
BBa_B0010
1
BBa_B0010
T1 from E. coli rrnB
2003-11-19T12:00:00Z
2015-08-31T04:07:20Z
Transcriptional terminator consisting of a 64 bp stem-loop.
false
false
_1_
0
24
7
In stock
false
true
Randy Rettberg
annotation7018
1
BBa_B0010
range7018
1
1
80
annotation4184
1
stem_loop
range4184
1
12
55
BBa_B0030
1
BBa_B0030
RBS.1 (strong) -- modified from R. Weiss
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
Strong RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>.
false
true
_44_46_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("orig" in figure 4-14 of Ron Weiss thesis). <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation7025
1
BBa_B0030
range7025
1
1
15
annotation1701
1
RBS-1\Strong
range1701
1
1
15
annotation1702
1
RBS
range1702
1
8
12
BBa_M1600
1
BBa_M1600
CXCR3
2012-03-26T11:00:00Z
2015-05-08T01:13:57Z
Aspergillus oryzae
putative type III polyketide synthase (PKS) gene
false
false
_768_
0
12289
9
Not in stock
false
na
false
Yoshitaro Heshiki
annotation2171438
1
Start
range2171438
1
1
3
annotation2171439
1
Coding Sequence
range2171439
1
1
1101
annotation2171440
1
Stop
range2171440
1
1099
1101
BBa_J23100
1
BBa_J23100
constitutive promoter family member
2006-08-03T11:00:00Z
2015-08-31T04:08:40Z
Isolated from library of promoters
Released HQ 2013
Replace later
false
true
_52_
0
483
95
In stock
true
N/A
true
John Anderson
BBa_M1601
1
BBa_M1601
CXCR3 Output Module
2012-03-27T11:00:00Z
2015-05-08T01:13:57Z
Constructed from Standard Registry promoter, rbs, and terminators. Coupled with previous biobrick (BBa_M1600).
This gene encodes a G protein-coupled receptor with selectivity for three chemokines, termed CXCL9/Mig (monokine induced by interferon-g), CXCL10/IP10 (interferon-g-inducible 10 kDa protein) and CXCL11/I-TAC (interferon-inducible T cell a-chemoattractant).
false
false
_768_
0
12289
9
Not in stock
false
NA
false
Yoshitaro Heshiki
component2223051
1
BBa_M1600
component2223052
1
BBa_B0010
component2223044
1
BBa_J23100
component2223054
1
BBa_B0012
component2223046
1
BBa_B0030
annotation2223054
1
BBa_B0012
range2223054
1
1262
1302
annotation2223052
1
BBa_B0010
range2223052
1
1174
1253
annotation2223051
1
BBa_M1600
range2223051
1
65
1165
annotation2223044
1
BBa_J23100
range2223044
1
1
35
annotation2223046
1
BBa_B0030
range2223046
1
44
58
BBa_M1601_sequence
1
ttgacggctagctcagtcctaggtacagtgctagctactagagattaaagaggagaaatactagatggtgctggaggttagcgaccatcaggtgctcaacgacgcagaggttgcggcgctgaattttagcagcagctatgactatggcgaaaacgagtcggattcatgctgcacttccccaccatgcccgcaggatttcagtctgaattttgaccgggcctttctgccggccttgtattcgctcttatttcttctgggtctgctcggtaacggcgctgtggctgcggttttattgagtcgtcggactgccttaagttctaccgacacatttcttcttcacttagcagttgcagataccctgctggttcttaccttgcccctgtgggctgtcgatgctgcggttcaatgggttttcggtagtggactttgtaaagttgcgggcgctctgtttaatattaatttctacgccggcgcgcttctgttggcctgtatttcctttgatcgttacttaaacattgtgcatgccacgcagctctatcggagaggaccgccggcacgcgtgacattgacgtgcctggctgtctggggtttgtgtctgctgtttgctctcccggattttatttttttatcagcccatcatgacgaacgtcttaatgcaacgcattgtcaatataattttccgcaagtgggtcggaccgccctccgcgtcttacagctcgtagcaggctttctgcttccgctgctggttatggcttattgttatgctcatattcttgcagtgttactggtttcacgtggacagcgccggctgcgcgctatgaggctcgttgtcgtagttgtggtagccttcgcgctctgttggacgccgtatcatctggttgtgctggttgatattctgatggacctgggcgccttagcgcgcaactgtggtcgggagagccgtgtagatgtggcaaaaagcgttacctccggtctaggttatatgcattgttgcttaaatccgctgctgtatgcgttcgtaggcgttaaatttagagaacgcatgtggatgctgctcctgcgactggggtgcccaaaccagcgtggcttacagcgtcagccgtcatcgtcccgccgggatagttcttggagcgaaacatctgaggcatcgtattcaggcttatagtactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_M1600_sequence
1
atggtgctggaggttagcgaccatcaggtgctcaacgacgcagaggttgcggcgctgaattttagcagcagctatgactatggcgaaaacgagtcggattcatgctgcacttccccaccatgcccgcaggatttcagtctgaattttgaccgggcctttctgccggccttgtattcgctcttatttcttctgggtctgctcggtaacggcgctgtggctgcggttttattgagtcgtcggactgccttaagttctaccgacacatttcttcttcacttagcagttgcagataccctgctggttcttaccttgcccctgtgggctgtcgatgctgcggttcaatgggttttcggtagtggactttgtaaagttgcgggcgctctgtttaatattaatttctacgccggcgcgcttctgttggcctgtatttcctttgatcgttacttaaacattgtgcatgccacgcagctctatcggagaggaccgccggcacgcgtgacattgacgtgcctggctgtctggggtttgtgtctgctgtttgctctcccggattttatttttttatcagcccatcatgacgaacgtcttaatgcaacgcattgtcaatataattttccgcaagtgggtcggaccgccctccgcgtcttacagctcgtagcaggctttctgcttccgctgctggttatggcttattgttatgctcatattcttgcagtgttactggtttcacgtggacagcgccggctgcgcgctatgaggctcgttgtcgtagttgtggtagccttcgcgctctgttggacgccgtatcatctggttgtgctggttgatattctgatggacctgggcgccttagcgcgcaactgtggtcgggagagccgtgtagatgtggcaaaaagcgttacctccggtctaggttatatgcattgttgcttaaatccgctgctgtatgcgttcgtaggcgttaaatttagagaacgcatgtggatgctgctcctgcgactggggtgcccaaaccagcgtggcttacagcgtcagccgtcatcgtcccgccgggatagttcttggagcgaaacatctgaggcatcgtattcaggcttatag
BBa_B0010_sequence
1
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
BBa_J23100_sequence
1
ttgacggctagctcagtcctaggtacagtgctagc
BBa_B0030_sequence
1
attaaagaggagaaa
BBa_B0012_sequence
1
tcacactggctcaccttcgggtgggcctttctgcgtttata
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z