BBa_M1654
1
BBa_M1654
S100 calcium biomarker
2013-03-31T11:00:00Z
2015-05-08T01:13:57Z
homo sapien
Homo sapiens S100 calcium binding protein A9 (S100A9), mRNA, biomarker
false
false
_768_
0
16144
9
Not in stock
false
codon optimization was done and restriction enzymes were removed
false
whitney morgan
annotation2214565
1
cds
range2214565
1
4
342
annotation2214564
1
stop
range2214564
1
343
345
annotation2214563
1
start
range2214563
1
1
3
BBa_B0011
1
BBa_B0011
LuxICDABEG (+/-)
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Derived from luxICDABEG operon terminator of Vibrio fischeri <genbank>AF170104</genbank>.
Released HQ 2013
Bidirectional transcriptional terminator consisting of a 22 bp stem-loop.</p>
false
false
_1_
0
24
7
In stock
false
<P> <P>In the naturally-occuring sequence there is a mismatch in the stem of the stem loop. This can be corrected via an A->G mutation (at position 40 -- sequence coordinate/not MFOLD coordinate). The above sequence does not reflect this mutation (but the MFOLD image does). This terminator's location cannot be found using some inverted repeat detectors like PALINDROME because it is too short and contains a mismatch. This one was found with the help of Tom Knight. It lies between two coding regions that point towards eachother.<P>
true
Reshma Shetty
annotation7019
1
BBa_B0011
range7019
1
1
46
annotation1683
1
stem_loop
range1683
1
13
35
BBa_J61101
1
BBa_J61101
Ribosome Binding Site Family Member
2007-01-28T12:00:00Z
2015-08-31T02:03:00Z
N/A
fix
false
false
_95_
0
483
95
In stock
false
N/A
true
John Anderson
BBa_M1652
1
BBa_M1652
CXC chemokine BRAK mRNA
2013-03-25T12:00:00Z
2015-05-08T01:13:57Z
Human source
Anti-marker protein
false
false
_768_
0
16132
9
Not in stock
false
None known currently
false
Howard Cordingley
annotation2214558
1
stop
range2214558
1
298
300
annotation2214559
1
CXC
range2214559
1
4
297
annotation2214557
1
start
range2214557
1
1
3
BBa_M1661
1
BBa_M1661
composite part for 1652 1654
2013-04-16T11:00:00Z
2015-05-08T01:13:57Z
Parts 1652 and 1654
composite part for coding of cxc chemokine marker and s100 calcium biomarker
false
false
_768_
0
16364
9
Not in stock
false
Add the restriction enzyme
false
Pratima labroo
component2215188
1
BBa_B0012
component2215179
1
BBa_J61101
component2215187
1
BBa_M1654
component2215167
1
BBa_K844000
component2215178
1
BBa_I712074
component2215162
1
BBa_I712074
component2215172
1
BBa_B0012
component2215163
1
BBa_J61101
component2215183
1
BBa_K844000
component2215171
1
BBa_M1652
component2215176
1
BBa_B0011
annotation2215179
1
BBa_J61101
range2215179
1
582
593
annotation2215172
1
BBa_B0012
range2215172
1
425
465
annotation2215167
1
BBa_K844000
range2215167
1
75
110
annotation2215176
1
BBa_B0011
range2215176
1
474
519
annotation2215163
1
BBa_J61101
range2215163
1
55
66
annotation2215183
1
BBa_K844000
range2215183
1
602
637
annotation2215178
1
BBa_I712074
range2215178
1
528
573
annotation2215171
1
BBa_M1652
range2215171
1
117
416
annotation2215187
1
BBa_M1654
range2215187
1
644
988
annotation2215162
1
BBa_I712074
range2215162
1
1
46
annotation2215188
1
BBa_B0012
range2215188
1
997
1037
BBa_I712074
1
BBa_I712074
T7 promoter (strong promoter from T7 bacteriophage)
2007-10-21T11:00:00Z
2015-08-31T04:07:46Z
T7 bacteriophage
T7 promoter is very specific promoter which is transcribed only by specific T7 RNA polymerase. Usually this promoter is used in expression systems where T7 promoter is cotransfected with T7 RNA polymerase. That ensures strong transcription of desired genes.
false
false
_130_
0
1856
9
In stock
false
true
Rok Gaber
BBa_K844000
1
BBa_K844000
10x-Histidine (10x-His) Tag with double stop codon (TAATAA)
2012-10-01T11:00:00Z
2015-05-08T01:13:33Z
Constructed through oligonucleotide annealing
Released HQ 2013
10x-Histidine tag with double stop codon TAATAA to allow for better extraction of tagged products and protein termination in a single part.
false
false
_1104_
0
9404
9
In stock
true
none
false
Kathleen Miller
annotation2206609
1
Stop
range2206609
1
34
36
annotation2206608
1
Stop
range2206608
1
31
33
annotation2206607
1
10x-Histidine Tag
range2206607
1
1
30
BBa_B0012
1
BBa_B0012
TE from coliphageT7
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
Released HQ 2013
Transcription terminator for the <i>E.coli</i> RNA polymerase.
false
false
_1_
0
24
7
In stock
false
<P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
false
Reshma Shetty
annotation1690
1
polya
range1690
1
28
41
annotation1686
1
T7 TE
range1686
1
8
27
annotation7020
1
BBa_B0012
range7020
1
1
41
annotation1687
1
stop
range1687
1
34
34
BBa_J61101_sequence
1
aaagacaggacc
BBa_M1654_sequence
1
atgacctgcaaaatgagccaactagaacgtaacattgagacgattatcaatacctttcatcagtattcagtgaaactggggcatccggataccctcaaccaaggcgagtttaaggaactggtaaggaaagacctgcaaaactttcttaaaaaagaaaataaaaatgaaaaagtcattgaacatattatggaggatttggataccaatgcagacaaacaactgtcttttgaagagttcattatgctcatggcgcgtctgacgtgggcgtctcatgaaaagatgcacgaaggtgatgaaggtccgggacaccaccacaagccggggctgggggaaggaactccctag
BBa_M1661_sequence
1
taatacgactcactatagggaatacaagctacttgttctttttgcatactagagaaagacaggacctactagagcatcatcaccatcaccaccatcatcaccattaataatactagatgcgtctgctggctgcggcactgttactgctgctgttggcgttgtataccgctcgcgttgatggcagtaaatgtaaatgttcacgcaaaggtccgaaaattcgctactctgatgtgaagaaactggaaatgaaaccgaaatatccgcactgtgaagaaaaaatggttatcatcaccacgaaaagcgttagccggtatcgcgggcaggaacactgcttgcatccgaaactccagtccaccaaacgctttatcaagtggtataatgcttggaatgaaaaaagacgcgtgtacgaggagtgatactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattttactagagtaatacgactcactatagggaatacaagctacttgttctttttgcatactagagaaagacaggacctactagagcatcatcaccatcaccaccatcatcaccattaataatactagatgacctgcaaaatgagccaactagaacgtaacattgagacgattatcaatacctttcatcagtattcagtgaaactggggcatccggataccctcaaccaaggcgagtttaaggaactggtaaggaaagacctgcaaaactttcttaaaaaagaaaataaaaatgaaaaagtcattgaacatattatggaggatttggataccaatgcagacaaacaactgtcttttgaagagttcattatgctcatggcgcgtctgacgtgggcgtctcatgaaaagatgcacgaaggtgatgaaggtccgggacaccaccacaagccggggctgggggaaggaactccctagtactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_I712074_sequence
1
taatacgactcactatagggaatacaagctacttgttctttttgca
BBa_K844000_sequence
1
catcatcaccatcaccaccatcatcaccattaataa
BBa_B0011_sequence
1
agagaatataaaaagccagattattaatccggcttttttattattt
BBa_B0012_sequence
1
tcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_M1652_sequence
1
atgcgtctgctggctgcggcactgttactgctgctgttggcgttgtataccgctcgcgttgatggcagtaaatgtaaatgttcacgcaaaggtccgaaaattcgctactctgatgtgaagaaactggaaatgaaaccgaaatatccgcactgtgaagaaaaaatggttatcatcaccacgaaaagcgttagccggtatcgcgggcaggaacactgcttgcatccgaaactccagtccaccaaacgctttatcaagtggtataatgcttggaatgaaaaaagacgcgtgtacgaggagtga
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z