LOCUS       BBa_M30100              2692 bp    DNA     linear       23-May-2024
DEFINITION  Dspelke: M13.1 - Refactored Genome
ACCESSION   BBa_M30100
VERSION     BBa_M30100.1  
FEATURES             Location/Qualifiers
     promoter        1144..1160
                     /label=promoter-g5
     promoter        381..428
                     /label=promoter-g10
     RBS             838..853
                     /label=rbs-g10
     RBS             1202..1217
                     /label=rbs-g5
     CDS             1218..1481
                     /label=g5 ORF
     misc_feature    1396..1400
                     /label=BsrGI
     RBS             1488..1503
                     /label=rbs-g7
     promoter        1557..1603
                     /label=promoter-g8
     RBS             1624..1639
                     /label=rbs-g9
     misc_feature    1702..1707
                     /label=SnaBI
     RBS             1751..1766
                     /label=rbs-g8
     CDS             1767..1988
                     /label=g8 ORF
     promoter        1966..2013
                     /label=promoter-g3
     RBS             2035..2050
                     /label=rbs-g3
     CDS             2051..2692
                     /label=g3 ORF (portion)
     misc_feature    2692..2692
                     /label=BamHI (cut)
     misc_feature    1..3
                     /label=HpaI (cut)
     CDS             1..831
                     /label=g2 ORF (portion)
     CDS             854..1189
                     /label=g10 ORF
     CDS             1510..1611
                     /label=g7 ORF
     CDS             1640..1738
                     /label=g9 ORF
     misc_feature    832..837
                     /label=AatII
     misc_feature    1190..1195
                     /label=ApaI
     misc_feature    1196..1201
                     /label=BmtI
     misc_feature    1482..1487
                     /label=EcoRI
     misc_feature    1504..1509
                     /label=KasI
     misc_feature    1612..1617
                     /label=NcoI
     misc_feature    1618..1623
                     /label=Nhe1
     misc_feature    1739..1744
                     /label=SalI
     misc_feature    1745..1750
                     /label=SpeI
     misc_feature    1989..1994
                     /label=XbaI
ORIGIN
        1 aacgctacta ctattagtag aattgatgcc accttttcag ctcgcgcccc aaatgaaaat
       61 atagctaaac aggttattga ccatttgcga aatgtatcta atggtcaaac taaatctact
      121 cgttcgcaga attgggaatc aactgttaca tggaatgaaa cttccagaca ccgtacttta
      181 gttgcatatt taaaacatgt tgagctacag caccagattc agcaattaag ctctaagcca
      241 tccgcaaaaa tgacctctta tcaaaaggag caattaaagg tactctctaa tcctgacctg
      301 ttggagtttg cttccggtct ggttcgcttt gaagctcgaa ttaaaacgcg atatttgaag
      361 tctttcgggc ttcctcttaa tctttttgat gcaatccgct ttgcttctga ctataatagt
      421 cagggtaaag acctgatttt tgatttatgg tcattctcgt tttctgaact gtttaaagca
      481 tttgaggggg attcaatgaa tatttatgac gattccgcag tattggacgc tatccagtct
      541 aaacatttta ctattacccc ctctggcaaa acttcttttg caaaagcctc tcgctatttt
      601 ggtttttatc gtcgtctggt aaacgagggt tatgatagtg ttgctcttac tatgcctcgt
      661 aattcctttt ggcgttatgt atctgcatta gttgaatgtg gtattcctaa atctcaactg
      721 atgaatcttt ctacctgtaa taatgttgtt ccgttagttc gttttattaa cgtagatttt
      781 tcttcccaac gtcctgactg gtataatgag ccagttctta aaatcgcata agacgtcatt
      841 tgagggggat tcaatgaata tttatgacga ttccgcagta ttggacgcta tccagtctaa
      901 acattttact attaccccct ctggcaaaac ttcttttgca aaagcctctc gctattttgg
      961 tttttatcgt cgtctggtaa acgagggtta tgatagtgtt gctcttacta tgcctcgtaa
     1021 ttccttttgg cgttatgtat ctgcattagt tgaatgtggt attcctaaat ctcaactgat
     1081 gaatctttct acctgtaata atgttgttcc gttagttcgt tttattaacg tagatttttc
     1141 ttcccaacgt cctgactggt ataatgagcc agttcttaaa atcgcataag ggcccgctag
     1201 ccataaggta attcacaatg attaaagttg aaattaaacc atctcaagcc caatttacta
     1261 ctcgttctgg tgtttctcgt cagggcaagc cttattcact gaatgagcag ctttgttacg
     1321 ttgatttggg taatgaatat ccggttcttg tcaagattac tcttgatgaa ggtcagccag
     1381 cctatgcgcc tggtctgtac accgttcatc tgtcctcttt caaagttggt cagttcggtt
     1441 cccttatgat tgaccgtctg cgcctcgttc cggctaagta agaattcgtt ccggctaagt
     1501 aacggcgcca tggagcaggt cgcggatttc gacacaattt atcaggcgat gatacaaatc
     1561 tccgttgtac tttgtttcgc gcttggtata atcgctgggg gtcaaagatg accatgggct
     1621 agctcgctgg gggtcaaaga tgagtgtttt agtgtattct ttcgcctctt tcgttttagg
     1681 ttggtgcctt cgtagtggca ttacgtattt tacccgttta atggaaactt cctcatgagt
     1741 cgacactagt taatggaaac ttcctcatga aaaagtcttt agtcctcaaa gcctctgtag
     1801 ccgttgctac cctcgttccg atgctgtctt tcgctgctga gggtgacgat cccgcaaaag
     1861 cggcctttaa ctccctgcaa gcctcagcga ccgaatatat cggttatgcg tgggcgatgg
     1921 ttgttgtcat tgtcggcgca actatcggta tcaagctgtt taagaaattc acctcgaaag
     1981 caagctgatc tagataaacc gatacaatta aaggctcctt ttggagcctt tttttttgga
     2041 gattttcaac gtgaaaaaat tattattcgc aattccttta gttgttcctt tctattctca
     2101 ctccgctgaa actgttgaaa gttgtttagc aaaaccccat acagaaaatt catttactaa
     2161 cgtctggaaa gacgacaaaa ctttagatcg ttacgctaac tatgagggtt gtctgtggaa
     2221 tgctacaggc gttgtagttt gtactggtga cgaaactcag tgttacggta catgggttcc
     2281 tattgggctt gctatccctg aaaatgaggg tggtggctct gagggtggcg gttctgaggg
     2341 tggcggttct gagggtggcg gtactaaacc tcctgagtac ggtgatacac ctattccggg
     2401 ctatacttat atcaaccctc tcgacggcac ttatccgcct ggtactgagc aaaaccccgc
     2461 taatcctaat ccttctcttg aggagtctca gcctcttaat actttcatgt ttcagaataa
     2521 taggttccga aataggcagg gggcattaac tgtttatacg ggcactgtta ctcaaggcac
     2581 tgaccccgtt aaaacttatt accagtacac tcctgtatca tcaaaagcca tgtatgacgc
     2641 ttactggaac ggtaaattca gagactgcgc tttccattct ggctttaatg ag
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