BBa_M36152 1 BBa_M36152 nifHDK promoter 2014-10-22T11:00:00Z 2015-05-08T01:14:03Z NifHDK sequence is downstream of nifA sequence and upstream of nifB sequence. Sequence determined from the genome of Azotobacter vinelandii. NifHDK promoter follows the NifA transcription factor and comprises three structural genes nifK,nif D, and nifH. nifK encodes for B-subunit of Component 1 of nitrogenase. nifD encodes for alpha subunit of component 1 of nitrogenase. nifH encodes for component 2 of nitrogenase. false false _848_ 0 24150 9 Not in stock false We selected the genomic region upstream of nifB but following nifA. This is the general region where the nifHDK promoter is found. false Monica Chin BBa_M36151 1 BBa_M36151 nifA transcription factor 2014-10-22T11:00:00Z 2015-05-08T01:14:03Z This sequence comes from the genome of Azotobacter vinelandii, specifically the nif cluster. NifA protein is nitrogen senitive and regulates transcription of nif genes. When there isn't enough fixed nitrogen available for the organism's use, NifA is expressed, and NifA activates the rest of the nif genes. If there is a sufficient amount of reduced nitrogen or oxygen is present, another protein is activated: NifL. NifL inhibits the action of NifA and results in inhibition of nitrogenase formation. false false _848_ 0 24150 9 Not in stock false NifA transcription factor corresponds with NifRLA promoter. false Monica Chin BBa_M36154 1 BBa_M36154 NifA sensor 2014-10-22T11:00:00Z 2015-05-08T01:14:03Z The sequences in this part come from the genome of Azotobacter vinelandii. This sensor functions by responding to nitrogen. The NifRLA promoter induces the transcription factor NifA. NifA regulates transcription of the rest of the nif gene. In this case, NifA activates the NifHDK promoter, which will interact with our reporter. false false _848_ 0 24150 9 Not in stock false In designing this part, we estimated the locations of the promoter sequences within the nif cluster. This may not be entirely accurate and may include extraneous sequences. false Monica Chin component2429014 1 BBa_M36152 component2429011 1 BBa_M36150 component2429013 1 BBa_M36151 component2429012 1 BBa_M36804 annotation2429011 1 BBa_M36150 range2429011 1 1 414 annotation2429014 1 BBa_M36152 range2429014 1 2019 2305 annotation2429012 1 BBa_M36804 range2429012 1 423 435 annotation2429013 1 BBa_M36151 range2429013 1 442 2010 BBa_M36804 1 BBa_M36804 pRBS-SD1+6A 2014-04-29T11:00:00Z 2015-05-08T01:14:06Z This part comes from GeneDesigner and DNA 2.0 This is a prokaryotic ribosome binding site from GeneDesigner (made by DNA 2.0). It has a 6A tail to provide spacing. false false _848_ 0 22143 9 Not in stock false No considerations made. false Bradley Hammoor BBa_M36150 1 BBa_M36150 nifRLA promoter 2014-10-22T11:00:00Z 2015-05-08T01:14:03Z The nifRLA promoter sequence was determined from the nif gene cluster sequence for Azotobacter vinelandii. It is found upstream of the nifL repressor. The nifRLA operon regulates the expression of the nitrogen fixation (nif) genes. NifA activates transcription of nif genes by the alternative form of RNA-polymerase, s54-holoenzyme. NifL is a negative regulatory gene which inhibits the activation of other nif genes by nifA protein. NifR is a repressor binding site, between the promoter of the nifRLA operon and the nifL gene. false false _848_ 0 24150 9 Not in stock false We selected the genomic region upstream of the nifL repressor but following the nifR repressor binding site. This is the general region where the nifRLA promoter is found. false Monica Chin BBa_M36152_sequence 1 tgacgggaccctccggcaatggatggccggctcctcccccctccccgttctccccgccccctcgtccgctacggacgcccgtccacgacggacatcgcctccgactcccgccacactgcttgtcgcttgtcgtacagaagcgcgcgaagggcggtgcggatttgtccgttgcgcgacaaattcggcccctcgttttcaaataaatcattcaaaaacaattacttgaataatcggcacgggtattgctcggctcttggggtaaagactctctcagccggagacagaac BBa_M36804_sequence 1 aggaggtaaaaaa BBa_M36150_sequence 1 ctgctcggctaaactttgtcgatgcgcctttgtcgggcgcagtctttatgccggaagttgtgcatcaagcatgccagcgctcaaaatttgcacaggcgtatcgcgggagcccctctaaaattgacctggatcaacaaatagcttcggcacgccagccgcctatccacccggcggccccggttttgtaaggtttgtgacagctcgttactgagcctgccgcccggcttgtgcgctttcgcacagctagagggcgaccaccccgaaaatccatgtttcgaggtttttccgagcaattcggcgcacccgggcgattaaggtgcggcacaggatttgctaatcttctctcaggcccaacacgcccctccggcggacgcagccgcgctcgccggttttcttggatagacgaggcacagc BBa_M36151_sequence 1 atgaacgcaacgattccgcagagaagcgcaaagcaaaacccggttgagctgtacgatctgcagctgcaagcgctggcgagcatcgctcgcacgctgagccgtgagcaacaaattgacgaactgttggaacaagtcctggcagttctgcacaacgatctgggtctgttacatggtctggttaccatcagcgacccggagcatggtgcgctgcagattggtgcgatccataccgactccgaagccgtggcgcaggcgtgtgagggcgtccgctaccgtagcggcgagggcgtcatcggtaacgtcctgaaacacggtaattcggtcgtgctgggtcgtattagcgcagacccacgctttttggaccgtctggccctgtatgacttggagatgccgttcatcgcagtgccgattaagaacccggaaggtaacactatcggtgtgctggctgcgcaacctgattgtcgcgcggacgaacacatgccggcacgcacgcgtctgctggaaattgtcgccaatctgctggcgcagaccgtgcgtctggttgtcaacatcgaagatggccgtgaagcggcagatgagcgtgacgagttgcgtcgcgaagttcgcggtaaatacggctttgagaatatggttgtcggtcatacgccgaccatgcgtcgtgtgttcgatcagattcgtcgcgtggcgaaatggaacagcacggttttggttctgggtgagtctggcaccggcaaagaattgatcgccagcgcgattcactataactccccgcgtgcgcaccgcccattcgtgcgtctgaattgcgctgcgctgccggaaacgctgctggagtccgagctgtttggtcacgagaaaggtgcgtttactggtgcagtgaagcaacgtaagggtcgttttgagcaagctgacggcggcaccctgttcctggatgaaatcggcgagattagcccgatgttccaggcgaaactgctgcgcgtcctgcaagagggcgagtttgagcgcgttggcggcaaccagaccgttcgtgtcaatgtgcgcatcgttgccgcgactaatcgcgacctggaaagcgaagtggagaaaggtaagttccgcgaagatctgtactaccgcttgaatgtgatggcaatccgtattccgccgctgcgcgagcgtaccgcagacattccggaactcgcagagttcttgctgggtaagatcggtcgtcaacagggccgtccgctgaccgttaccgacagcgcgatccgtctgcttatgagccatcgttggccgggtaacgttcgtgagctggagaattgcctggagcgtagcgccattatgagcgaagatggcacgattacccgtgacgtcgtgagcctgaccggtgtcgataacgaatctccgccgctggcggcaccgctgcctgaagtcaatctggccgatgaaacgctggacgatcgtgagcgcgttatcgcggcactggaacaggctggctgggttcaggcgaaagccgctcgtctgttgggtatgaccccgcgtcaaatcgcctatcgcattcagaccctgaacattcacatgcgtaagatctaa BBa_M36154_sequence 1 ctgctcggctaaactttgtcgatgcgcctttgtcgggcgcagtctttatgccggaagttgtgcatcaagcatgccagcgctcaaaatttgcacaggcgtatcgcgggagcccctctaaaattgacctggatcaacaaatagcttcggcacgccagccgcctatccacccggcggccccggttttgtaaggtttgtgacagctcgttactgagcctgccgcccggcttgtgcgctttcgcacagctagagggcgaccaccccgaaaatccatgtttcgaggtttttccgagcaattcggcgcacccgggcgattaaggtgcggcacaggatttgctaatcttctctcaggcccaacacgcccctccggcggacgcagccgcgctcgccggttttcttggatagacgaggcacagctactagagaggaggtaaaaaatactagatgaacgcaacgattccgcagagaagcgcaaagcaaaacccggttgagctgtacgatctgcagctgcaagcgctggcgagcatcgctcgcacgctgagccgtgagcaacaaattgacgaactgttggaacaagtcctggcagttctgcacaacgatctgggtctgttacatggtctggttaccatcagcgacccggagcatggtgcgctgcagattggtgcgatccataccgactccgaagccgtggcgcaggcgtgtgagggcgtccgctaccgtagcggcgagggcgtcatcggtaacgtcctgaaacacggtaattcggtcgtgctgggtcgtattagcgcagacccacgctttttggaccgtctggccctgtatgacttggagatgccgttcatcgcagtgccgattaagaacccggaaggtaacactatcggtgtgctggctgcgcaacctgattgtcgcgcggacgaacacatgccggcacgcacgcgtctgctggaaattgtcgccaatctgctggcgcagaccgtgcgtctggttgtcaacatcgaagatggccgtgaagcggcagatgagcgtgacgagttgcgtcgcgaagttcgcggtaaatacggctttgagaatatggttgtcggtcatacgccgaccatgcgtcgtgtgttcgatcagattcgtcgcgtggcgaaatggaacagcacggttttggttctgggtgagtctggcaccggcaaagaattgatcgccagcgcgattcactataactccccgcgtgcgcaccgcccattcgtgcgtctgaattgcgctgcgctgccggaaacgctgctggagtccgagctgtttggtcacgagaaaggtgcgtttactggtgcagtgaagcaacgtaagggtcgttttgagcaagctgacggcggcaccctgttcctggatgaaatcggcgagattagcccgatgttccaggcgaaactgctgcgcgtcctgcaagagggcgagtttgagcgcgttggcggcaaccagaccgttcgtgtcaatgtgcgcatcgttgccgcgactaatcgcgacctggaaagcgaagtggagaaaggtaagttccgcgaagatctgtactaccgcttgaatgtgatggcaatccgtattccgccgctgcgcgagcgtaccgcagacattccggaactcgcagagttcttgctgggtaagatcggtcgtcaacagggccgtccgctgaccgttaccgacagcgcgatccgtctgcttatgagccatcgttggccgggtaacgttcgtgagctggagaattgcctggagcgtagcgccattatgagcgaagatggcacgattacccgtgacgtcgtgagcctgaccggtgtcgataacgaatctccgccgctggcggcaccgctgcctgaagtcaatctggccgatgaaacgctggacgatcgtgagcgcgttatcgcggcactggaacaggctggctgggttcaggcgaaagccgctcgtctgttgggtatgaccccgcgtcaaatcgcctatcgcattcagaccctgaacattcacatgcgtaagatctaatactagagtgacgggaccctccggcaatggatggccggctcctcccccctccccgttctccccgccccctcgtccgctacggacgcccgtccacgacggacatcgcctccgactcccgccacactgcttgtcgcttgtcgtacagaagcgcgcgaagggcggtgcggatttgtccgttgcgcgacaaattcggcccctcgttttcaaataaatcattcaaaaacaattacttgaataatcggcacgggtattgctcggctcttggggtaaagactctctcagccggagacagaac igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z