BBa_M36629

BBa_M36629 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_M36629
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Benjamin Yeh, Jodie Sheffels, Ali Hoffer
Date created: 2015-10-23 11:00:00
Date modified: 2015-10-24 05:34:12

FLAG Epitope



Types
DnaRegion

Roles
Reporter

engineered_region

Sequences BBa_M36629_sequence (Version 1)

Description

Encodes the FLAG epitope, which has the amino acid sequence of DYKDDDDK. Can be added to either the N- or C- terminus of a protein. No start codon included.

Notes

1. Avoid BsaI, BbsI, and BsmBI restriction sites
2. Avoid repeats over 10bp in length.

Source

The DNA sequence was generated from the amino acid sequence (DYKDDDDK) using IDT Codon Optimizer for E. coli K12. It was then modified to remove certain restriction sites and repeats.

igem#experience
None
 
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_M36629/1