BBa_M36659 1 BBa_M36659 Poly-3-hydroxybutyrate Growth Actuator 2015-10-22T11:00:00Z 2015-12-04T06:55:33Z Xanthomonas campestris pv. raphani 756C is the organism from which the DNA sequence for poly-beta-hydroxybutyrate polymerase. References include http://www.uniprot.org/uniprot/G0CFI5 and http://www.google.com/patents/WO1995020621A1?cl=en. Part codes for the mRNA sequence to produce poly-beta-hydroxybutyrate polymerase, which synthesizes poly-3-hydroxybutyrate in the presence of glucose. This composite part is simply the DNA sequence collected from Xanthomonas campestris pv. raphani 756C, specifically from the gene G0CFI5. false false _848_ 29108 29108 9 false Design considerations include limiting the DNA sequence to 1500 bp, avoiding BbsI, BsaI, and BsmBI, and ensuring less than five repeats of 10 bp. Restriction sites were avoided to not conflict with DNA 2.0 synthesis process, as was the minimization of repeats in the sequence. false Taylor Marie Chavez, Maria Iglesias, Alison Jahansouz component2478433 1 BBa_M36658 component2478432 1 BBa_M36660 annotation2478432 1 BBa_M36660 range2478432 1 1 1077 annotation2478433 1 BBa_M36658 range2478433 1 1084 1806 BBa_M36658 1 BBa_M36658 mRNA Sequence for Acetoacetyl-CoA Reductase 2015-12-04T12:00:00Z 2015-12-04T06:52:29Z Part comes from the genomic sequence of Phaeobacter inhibens (strain ATCC 700781/DSM 17395/CIP 105210/NBRC 16654/BS107). Referenced http://www.uniprot.org/uniprot/G0CFI5 and http://www.google.com/patents/WO1995020621A1?cl=en, which both contributed to the discovery of the gene sequence for the necessary protein. Part codes for the mRNA sequence to produce acetoacetyl-CoA reductase, which synthesizes poly-3-hydroxybutyrate in from acetoacetyl-CoA. This composite part is the DNA sequence collected from an amino acid sequence obtained from Phaeobacter inhibens (strain ATCC 700781/DSM 17395/CIP 105210/NBRC 16654/BS107), specifically from the gene I7EIV2. Part is intended to be inserted into pD454-GST, with a high copy number and ampicillin resistance. Complete system should be transformed into E. Coli. false false _848_ 29108 29108 9 false Part was designed to meet the following conditions: be less than 1500 bp, avoid BbsI, BsaI, and BsmBI, and have less than five repeats 10 bp. There was an active attempt to avoid the restriction sites, stated above, that DNA 2.0 uses in the synthesis of DNA and to minimize repeats in the sequence to ensure success during synthesis. A limit of 1500 bp was imposed by the course. false Taylor Marie Chavez, Maria Iglesias, Alison Jahansouz BBa_M36660 1 BBa_M36660 mRNA Sequence for Poly-beta-Hydroxybutyrate Polymerase 2015-10-22T11:00:00Z 2015-12-04T06:52:56Z Part comes from the genomic sequence of Xanthomonas campestris pv. raphani 756C, specifically G0CFI5. Referenced http://www.uniprot.org/uniprot/G0CFI5 and http://www.google.com/patents/WO1995020621A1?cl=en, which both contributed to the discovery of the gene sequence for the necessary protein. Part codes for the mRNA sequence to produce poly-beta-hydroxybutyrate polymerase, which synthesizes poly-3-hydroxybutyrate in the presence of glucose. Poly-3-hydroxybutyrate, or P3HB, is a biodegradable polymer which will break down after it has fulfilled its intended use. Part is intended to be inserted into pD431-SR, with a high copy number and kanamycin resistance. Complete system should be transformed into E. Coli. Amino Acid sequence is as follows: MKGPLGFSAEDLMQETLSMQRKLREGLKLLPGVEDVDYGVTERQEVWRDGKVVLYRFVGDAAPVARTPLLIVYALVNRPYMVDLQADRSLVKGLLGHGQDVYVLDWGYPDRSERYLTLEDYLLRYIDGAVDHLRAASGLEAVDVLGICQGGTFALCYAALERAKIRNLITMVTPVDFHTPDNMLSNWARMVDVDLFVDTMGNVPADLMNASYLMLKPFRLNLQKYVGLLDILDDKQALEDFLRMEKWIFDSPDLAGEAFREFVTQFYQRNGLVTGDVRIGGQAVDLHAVDMPVLNIYAEHDHLVPPDASRALRGLVGSEDYTELSFRGGHIGIYVSGRAQREVPVAIHRWLQARGGNG* false false _848_ 29108 29108 9 false Part was designed to meet the following conditions: be less than 1500 bp, avoid BbsI, BsaI, and BsmBI, and have less than five repeats 10 bp. There was an active attempt to avoid the restriction sites, stated above, that DNA 2.0 uses in the synthesis of DNA and to minimize repeats in the sequence to ensure success during synthesis. A limit of 1500 bp was imposed by the course. false Taylor Marie Chavez, Maria Iglesias, Alison Jahansouz BBa_M36660_sequence 1 atgaagggtcctcttggtttttctgccgaagatttgatgcaagagacgttgtccatgcagcgtaaactgcgtgaaggcctgaaactcctgcctggtgtggaggatgttgactatggtgttactgaacgccaggaagtttggcgcgacggcaaagtcgtcttgtatcgtttcgtcggagacgcggcaccagttgcacgtacaccgttgctgattgtgtatgctttagttaatcgtccttacatggtagatctgcaggccgaccgttccctcgttaaaggtctgttgggccatggccaggatgtgtatgtgctggattggggttatccggatcgttccgaacgttatctgacgctggaagattatttacttcgctacatcgatggcgccgttgaccatcttcgcgctgcaagtggtctggaggcggttgatgttctgggtatctgccaaggtgggacgtttgctctctgctacgcagcattagaacgcgctaaaatccgcaatctgatcaccatggtgaccccagtcgacttccacaccccggacaatatgttatcgaactgggcccgcatggtggacgttgatctgtttgtggataccatggggaatgtaccggcggatttaatgaacgcgagctatctcatgctgaaaccgttccgcctgaacctccaaaagtatgtgggattactggatattctcgatgacaagcaggccttagaagacttcctgcgtatggaaaaatggatctttgatagccccgacctcgcgggcgaagcattccgtgagttcgtgacccagttttatcagcgtaatggcctggtgaccggcgatgtccgtattggcggtcaagctgtggatctgcacgcagtggatatgccggtcctgaacatctatgcagaacatgatcatctggttccgccggacgcttctcgcgcgctgcgtggacttgtcgggtcggaagattataccgaactgtcatttcgtggagggcacattggtatttatgtctcggggcgcgcccagcgcgaagtgcctgtagctattcatcgttggttacaggcccgcggtggtaacggttga BBa_M36659_sequence 1 atgaagggtcctcttggtttttctgccgaagatttgatgcaagagacgttgtccatgcagcgtaaactgcgtgaaggcctgaaactcctgcctggtgtggaggatgttgactatggtgttactgaacgccaggaagtttggcgcgacggcaaagtcgtcttgtatcgtttcgtcggagacgcggcaccagttgcacgtacaccgttgctgattgtgtatgctttagttaatcgtccttacatggtagatctgcaggccgaccgttccctcgttaaaggtctgttgggccatggccaggatgtgtatgtgctggattggggttatccggatcgttccgaacgttatctgacgctggaagattatttacttcgctacatcgatggcgccgttgaccatcttcgcgctgcaagtggtctggaggcggttgatgttctgggtatctgccaaggtgggacgtttgctctctgctacgcagcattagaacgcgctaaaatccgcaatctgatcaccatggtgaccccagtcgacttccacaccccggacaatatgttatcgaactgggcccgcatggtggacgttgatctgtttgtggataccatggggaatgtaccggcggatttaatgaacgcgagctatctcatgctgaaaccgttccgcctgaacctccaaaagtatgtgggattactggatattctcgatgacaagcaggccttagaagacttcctgcgtatggaaaaatggatctttgatagccccgacctcgcgggcgaagcattccgtgagttcgtgacccagttttatcagcgtaatggcctggtgaccggcgatgtccgtattggcggtcaagctgtggatctgcacgcagtggatatgccggtcctgaacatctatgcagaacatgatcatctggttccgccggacgcttctcgcgcgctgcgtggacttgtcgggtcggaagattataccgaactgtcatttcgtggagggcacattggtatttatgtctcggggcgcgcccagcgcgaagtgcctgtagctattcatcgttggttacaggcccgcggtggtaacggttgatactagatggcccgtaacgcgctggtcaccggcggtagtcgcgggatcggagcagccatctcacaggcgctgaaagcggagggctacaccgtggccgctacctacgctggcaatgacgaagcggcggcgaaattcacgaatgagactggcattaaaacatataaatgggatgttgcgagctatgaagattcggccgccggtatcgccaaggttgaagcggacattgggccgattgatattgttgtggcaaatgctgggattacccgtgatgctcccttccataaaatgacgctcgagcagtggcagcaggtcatagataccaacctcaccggtgtgtttaacaccgtgcatccgatctggcctggtatgcgcgaacgcaagttcggccgtgtgatcgtcatttcttccatcaacgggcaaaaagggcagtttgcgcaggtaaattatgcggctactaaagccggtgatcttggtatcgtgaaatccctggcgcaagaaggagctcgcgcgggcatcaccgccaatgccatttgccccggttatatcgcgacagaaatggtcatggccgttcctgaaaaggttcgtgagagcattatcggccagattcctgccggacgtttaggcgaacccgaagaaattgctcgttgcgtggcattcttggcgtccgaggactcgggctttattaacgggtctacaatcagcgcgaatggcgcccagtttttcgtataa BBa_M36658_sequence 1 atggcccgtaacgcgctggtcaccggcggtagtcgcgggatcggagcagccatctcacaggcgctgaaagcggagggctacaccgtggccgctacctacgctggcaatgacgaagcggcggcgaaattcacgaatgagactggcattaaaacatataaatgggatgttgcgagctatgaagattcggccgccggtatcgccaaggttgaagcggacattgggccgattgatattgttgtggcaaatgctgggattacccgtgatgctcccttccataaaatgacgctcgagcagtggcagcaggtcatagataccaacctcaccggtgtgtttaacaccgtgcatccgatctggcctggtatgcgcgaacgcaagttcggccgtgtgatcgtcatttcttccatcaacgggcaaaaagggcagtttgcgcaggtaaattatgcggctactaaagccggtgatcttggtatcgtgaaatccctggcgcaagaaggagctcgcgcgggcatcaccgccaatgccatttgccccggttatatcgcgacagaaatggtcatggccgttcctgaaaaggttcgtgagagcattatcggccagattcctgccggacgtttaggcgaacccgaagaaattgctcgttgcgtggcattcttggcgtccgaggactcgggctttattaacgggtctacaatcagcgcgaatggcgcccagtttttcgtataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z