| Types | DnaRegion
|
| Roles | Other
sequence_feature
|
| Sequences | BBa_M36676_sequence (Version 1)
|
Description
attB and attP are identified by Bxb1 integrase to determine the bounds for cutting. For an invertase switch, place attP, then the promoter of interest upstream, then att. upstream of the gene. When used in an invertase switch, Bxb1 cuts the intervening code and substitutes attL and attR sites at attB and attP, respectively. Bxb1 will not cut attL and attP sites without additional cofactors.
Notes
Similar sequences already exist on parts registry- this may not be the most condensed sequence available.
Source
http://www.sciencedirect.com/science/article/pii/S0022283603013561
