Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Shankara Anand
Date created: 2014-10-23 11:00:00
Date modified: 2015-05-08 01:14:05
HSE 2x Promoter + Bxb1 Recombinase w/ NLS + SV40 Terminator + attB site + CMV Reverse + attP site
| Types | DnaRegion |
| Roles | Composite engineered_region |
| Sequences | BBa_M36689_sequence (Version 1) |
Description
This part incorporates a HSE 2x optimized promoter for a highly elucidated heat shock transcription factor. Once this site has been activated, it signals a Bxb1 Recombinase, a serine based recombinase. When used as an invertase, Bxb1 identifies attB and attP sites, cuts at these sites, and inverts the DNA sequence in between these two markers. Bb1 integrase is capable of performing this process without additional cofactors, which has been confirmed by in vitro studies. The attB and attP sites are both incorporated into this composite with a CMV reverse complement promoter within. This reverse promoter will, as a result, be switched "on" once activated by the HSE 2x transcription factor site and may be used to activated some sort of sensor.Notes
This composite was designed for use in mammalian cells. As a result, we've incorporated an NLS within our Bxb1 Recombinase to ensure compatibility. Additionally, our serine based recombinase used here has been proven in studies to work properly with our attB and attP recognition sites.Source
HSE 2x: http://parts.igem.org/Part:BBa_M36765; http://www.ncbi.nlm.nih.gov/pubmed/25168173Bxb1 Integrase: http://parts.igem.org/Part:BBa_M36670; http://parts.igem.org/Part:BBa_K907000
attB & attP: http://link.springer.com/article/10.1007/s00438-006-0129-5
CMV Reverse Complement: http://parts.igem.org/Part:BBa_M36769; http://parts.igem.org/Part:BBa_I712004
| Access | Instance | Definition |
| public public public public public public | BBa_M36765 BBa_M36670 BBa_J52016 BBa_M36676 BBa_M36769 BBa_M36864 | BBa_M36765 BBa_M36670 BBa_J52016 BBa_M36676 BBa_M36769 BBa_M36864 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_M36765 BBa_M36670 BBa_J52016 BBa_M36676 BBa_M36769 BBa_M36864 | 1,98 105,1628 1637,1874 1883,1922 1931,2519 2528,2567 | BBa_M36765 BBa_M36670 BBa_J52016 BBa_M36676 BBa_M36769 BBa_M36864 |