BBa_M36765

BBa_M36765 Version 1

Component

Source: http://parts.igem.org/Part:BBa_M36765

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Shankara Anand

Date created: 2014-10-23 11:00:00

Date modified: 2015-05-08 01:14:06

Optimized Heat Shock Promoter (2x HSE)

Details

• Types: DnaRegion
• Roles: promoter; Regulatory
• Sequences: BBa_M36765_sequence (Version 1)

Description

This part is an optimized Heat Shock Expression (HSE) promoter that has been developed as a replacement for HSPA1a, a common promoter used for detection of the transcription factor HSF1. These Heat Shock Proteins (HSPs) are crucial for preventing cell death/enhancing survival. In the study where this sequence was developed, the authors describe a repeating sequence of HSE elements, measuring the change in response with respect to number of elements incorporated. Although 5 HSE's were identified to optimize HSP expression, this sequence uses 2 HSE elements.

These HSE's are more efficient due to selective detection of Heat Shock pathway activity as opposed to their HSPA1a counterparts which have other confounding stress factors included.

Notes

Because we needed to synthesize this sequence, we only used 2x HSE's due to the repetitive nature of this promoter. If users would like a stronger heat shock recognizing promoter, refer to the source provided.

Source

http://www.ncbi.nlm.nih.gov/pubmed/25168173

Other Properties

• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_M36765/1

Member of these Collections

• iGEM Parts Registry