LOCUS       BBa_M36980              1333 bp    DNA     linear       21-May-2024
DEFINITION  Coenzyme B-12 Biosensor for E. coli (
ACCESSION   BBa_M36980
VERSION     BBa_M36980.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23100
     RBS             253..280
                     /label=Shine-Dalgarno Sequence
     misc_feature    185..202
                     /label=B12 box/B12 element
     misc_feature    44..283
                     /label=BBa_M36998
     CDS             290..469
                     /label=lacZa
     misc_feature    470..526
                     /label=linker
     CDS             527..1243
                     /label=GFP
     misc_feature    290..1243
                     /label=BBa_E0051
     terminator      1252..1333
                     /label=BBa_M36010
ORIGIN
        1 ttgacggcta gctcagtcct aggtacagtg ctagctacta gaggccggtc ctgtgagtta
       61 atagggaatc cagtgcgaat ctggagctga cgcgcagcgg taaggaaagg tgcgatgatt
      121 gcgttatgcg gacactgcca ttcggtggga agtcatcatc tcttagtatc ttagataccc
      181 ctccaagccc gaagacctgc cggccaacgt cgcatctggt tctcatcatc gcgtaatatt
      241 gatgaaacct gcggcatcct tcttctattg tggatgcttt acatactaga tgaccatgat
      301 tacggattca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca
      361 acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg
      421 caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatggcgcg cgggcggcag
      481 cgaaggcggc ggcagcgaac atcatcatca tcatcatggc agcgaacgta aaggagaaga
      541 acttttcact ggagttgtcc caattcttgt tgaattagat ggtgatgtta atgggcacaa
      601 attttctgtc agtggagagg gtgaaggtga tgcaacatac ggaaaactta cccttaaatt
      661 tatttgcact actggaaaac tacctgttcc atggccaaca cttgtcacta ccctgaccta
      721 tggtgttcaa tgctttgcga gatacccaga tcatatgaaa cagcatgact ttttcaagag
      781 tgccatgccc gaaggttatg tacaggaaag aactatattt ttcaaagatg acgggaacta
      841 caagacacgt gctgaagtca agtttgaagg tgataccctt gttaatagaa tcgagttaaa
      901 aggtattgat tttaaagaag atggaaacat tcttggacac aaattggaat acaactataa
      961 ctcacacaat gtatacatca tggcagacaa acaaaagaat ggaatcaaag ttaacttcaa
     1021 aattagacac aacattgaag atggaagcgt tcaactagca gaccattatc aacaaaatac
     1081 tccaattggc gatggccctg tccttttacc agacaaccat tacctgtcca cacaatctgc
     1141 cctttcgaaa gatcccaacg aaaagagaga ccacatggtc cttcttgagt ttgtaacagc
     1201 tgctgggatt acacatggca tggatgaact atacaaataa taatactaga gtcggtcagt
     1261 ttcacctgat ttacgtaaaa acccgcttcg gcgggttttt gcttttggag gggcagaaag
     1321 atgaatgact gtc
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