BBa_M36009
1
BBa_M36009
5' Bicistronic UTR (strong), does not include ATG start
2011-04-25T11:00:00Z
2015-05-08T01:14:02Z
c/o Vivek Mutalik et al. BIOFAB Emeryville
5' UTR based on bicistronic junction architecture. First RBS is strong and drives expression of short leader cistron. Second RBS internal to first cistron control translation of gene of interest. Architecture is optimized so that downstream CDS for gene of interest does not have a chance to form a secondary structure with the 5' UTR. See BIOFAB.org for more information.
false
false
_848_
0
52
432
Not in stock
false
please see long description.
false
Drew Endy
BBa_M36994
1
BBa_M36994
Type II Secreted Antifreeze Actuator in E. coli (1-Repeat)
2011-04-27T11:00:00Z
2015-05-08T01:14:07Z
Based on a protein sequence from the white flounder, Pseudopleuronecta americanus.
5'UTR, signal peptide, non-glycolated antifreeze protein, and terminator that will as whole transcribe and secrete antifreeze protein outside the cell membrane.
true
false
_848_
0
9543
9
Discontinued
false
This design has a "ANAAAAAALTA" sequence that is only roughly used once, unlike other AFP sequences which have more repeats of this sequence.
false
Nathan Barnett, Meghan Bowler, Kian Torabian
component2118487
1
BBa_M36009
component2118490
1
BBa_M36010
component2118488
1
BBa_M36726
component2118489
1
BBa_M36997
annotation2118487
1
BBa_M36009
range2118487
1
1
47
annotation2118489
1
BBa_M36997
range2118489
1
130
231
annotation2118490
1
BBa_M36010
range2118490
1
240
321
annotation2118488
1
BBa_M36726
range2118488
1
56
121
BBa_M36726
1
BBa_M36726
Type II Signal Peptide
2011-04-27T11:00:00Z
2015-05-08T01:14:06Z
This peptide is a genomic sequence from E. coli.
An N-terminus peptide that carries and delivers a protein outside of the cell membrane using the Sec Pathway.
false
false
_848_
0
9543
9
Not in stock
false
This is an N-terminal protein that should precede the protein you would like exported.
false
Nathan Barnett, Meghan Bowler, Kian Torabian
annotation2119866
1
start
range2119866
1
1
3
BBa_M36997
1
BBa_M36997
Non-Glycolated Antifreeze Protein (AFP) - 1-Repeated Segment
2011-04-27T11:00:00Z
2016-01-27T10:45:22Z
Comes from the white flounder, Pseudopleuronecta americanus.
The standard non-glycolated antifreeze protein (AFP) from the Pseudopleuronecta americanus. The codons have been optimized for E. coli.
false
false
_848_
4206
9543
9
Not in stock
false
This is a reduced segment of the 5-Repeated Segment AFP, with four "ANAAAAAALTA" taken out.
false
Nathan Barnett, Meghan Bowler, Kian Torabian
annotation2119812
1
c-linker
range2119812
1
1
9
annotation2119815
1
Unit 1 (TAANAAAAAAL)
range2119815
1
46
78
annotation2119814
1
Modified Unit 0 (TASDAAAAAL)
range2119814
1
16
45
annotation2119816
1
stop
range2119816
1
91
96
annotation2119813
1
start
range2119813
1
10
12
BBa_M36010
1
BBa_M36010
Transcription Terminator (Strong)
2011-04-25T11:00:00Z
2015-05-08T01:14:02Z
Guillaume Cambray et al. c/o BIOFAB Emeryville
E. coli RNA pol transcription terminator designed and tested by Guillaume Cambray at BIOFAB Emeryville. Based on the natural E. coli rnpB T1 terminator.
false
true
_848_
0
52
432
Not in stock
false
please see long description
false
Drew Endy
BBa_M36994_sequence
1
tatagagggtattaataatgtatggattaaaaggggaggtataacaatactagaggcgaccaaactggtgctgggcgcggtgattctgggcagcaccctgctggcgggctgcagcagcaactactagagcccccccccatggacactgcctctgacgccgcagctgcagcactgacagcggctaatgctgctgcagcagcagctttgactgcacggtaataggatccgcgctactagagtcggtcagtttcacctgatttacgtaaaaacccgcttcggcgggtttttgcttttggaggggcagaaagatgaatgactgtc
BBa_M36726_sequence
1
gcgaccaaactggtgctgggcgcggtgattctgggcagcaccctgctggcgggctgcagcagcaac
BBa_M36010_sequence
1
tcggtcagtttcacctgatttacgtaaaaacccgcttcggcgggtttttgcttttggaggggcagaaagatgaatgactgtc
BBa_M36009_sequence
1
tatagagggtattaataatgtatggattaaaaggggaggtataacaa
BBa_M36997_sequence
1
cccccccccatggacactgcctctgacgccgcagctgcagcactgacagcggctaatgctgctgcagcagcagctttgactgcacggtaataggatccgcgc
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z