LOCUS       BBa_M39412              1347 bp    DNA     linear       13-May-2024
DEFINITION  DNAseI generator inhibited by recombinant pCat promoter
ACCESSION   BBa_M39412
VERSION     BBa_M39412.1  
FEATURES             Location/Qualifiers
     stem_loop       12..55
                     /label=stem_loop
     misc_feature    1..80
                     /label=BBa_B0010
     terminator      1..80
                     /label=BBa_B0010
     stem_loop       96..115
                     /label=T7 TE
     misc_feature    122..122
                     /label=stop
     polyA_site      116..129
                     /label=polya
     misc_feature    89..129
                     /label=BBa_B0012
     terminator      89..129
                     /label=BBa_B0012
     terminator      1..129
                     /label=BBa_B0015
     CDS             139..755
                     /label=tetR
     misc_feature    756..789
                     /label=SsrA
     misc_feature    796..820
                     /label=Help:Barcodes
     CDS             136..820
                     /label=BBa_C0040
     RBS             829..840
                     /label=BBa_J61106
     RBS             849..860
                     /label=BBa_J61105
     RBS             869..880
                     /label=BBa_J61104
     misc_feature    889..905
                     /label=IRL in
     misc_feature    907..923
                     /label=IRL out
     misc_feature    889..923
                     /label=BBa_K137010
     stem_loop       943..986
                     /label=stem_loop
     misc_feature    932..1011
                     /label=BBa_B0010
     terminator      932..1011
                     /label=BBa_B0010
     stem_loop       1027..1046
                     /label=T7 TE
     misc_feature    1053..1053
                     /label=stop
     polyA_site      1047..1060
                     /label=polya
     misc_feature    1020..1060
                     /label=BBa_B0012
     terminator      1020..1060
                     /label=BBa_B0012
     terminator      932..1060
                     /label=BBa_B0015
     promoter        1072..1077
                     /label=-35
     promoter        1095..1100
                     /label=-10
     misc_feature    1069..1106
                     /label=P(Cat)
     promoter        1069..1106
                     /label=BBa_I14033
     misc_feature    1115..1131
                     /label=IRR out
     misc_feature    1133..1149
                     /label=IRR in
     misc_feature    1115..1149
                     /label=BBa_K137008
     RBS             1158..1169
                     /label=BBa_J61103
     misc_feature    1178..1210
                     /label=WT SsrA tag AANDENYALAA
     misc_feature    1178..1210
                     /label=BBa_M0050
     stem_loop       1230..1273
                     /label=stem_loop
     misc_feature    1219..1298
                     /label=BBa_B0010
     terminator      1219..1298
                     /label=BBa_B0010
     stem_loop       1314..1333
                     /label=T7 TE
     misc_feature    1340..1340
                     /label=stop
     polyA_site      1334..1347
                     /label=polya
     misc_feature    1307..1347
                     /label=BBa_B0012
     terminator      1307..1347
                     /label=BBa_B0012
     terminator      1219..1347
                     /label=BBa_B0015
ORIGIN
        1 ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt
       61 gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct
      121 gcgtttatat actagatgtc cagattagat aaaagtaaag tgattaacag cgcattagag
      181 ctgcttaatg aggtcggaat cgaaggttta acaacccgta aactcgccca gaagctaggt
      241 gtagagcagc ctacattgta ttggcatgta aaaaataagc gggctttgct cgacgcctta
      301 gccattgaga tgttagatag gcaccatact cacttttgcc ctttagaagg ggaaagctgg
      361 caagattttt tacgtaataa cgctaaaagt tttagatgtg ctttactaag tcatcgcgat
      421 ggagcaaaag tacatttagg tacacggcct acagaaaaac agtatgaaac tctcgaaaat
      481 caattagcct ttttatgcca acaaggtttt tcactagaga atgcattata tgcactcagc
      541 gctgtggggc attttacttt aggttgcgta ttggaagatc aagagcatca agtcgctaaa
      601 gaagaaaggg aaacacctac tactgatagt atgccgccat tattacgaca agctatcgaa
      661 ttatttgatc accaaggtgc agagccagcc ttcttattcg gccttgaatt gatcatatgc
      721 ggattagaaa aacaacttaa atgtgaaagt gggtccgctg caaacgacga aaactacgct
      781 ttagtagctt aataacactg atagtgctag tgtagatcac tactagagaa agataggaga
      841 tactagagaa agacatgacg tactagagaa agaagggaca tactagagtc tatgagtcaa
      901 aatggcccca attgtcttgt atttactaga gccaggcatc aaataaaacg aaaggctcag
      961 tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct ctactagagt
     1021 cacactggct caccttcggg tgggcctttc tgcgtttata tactagaggg cacgtaagag
     1081 gttccaactt tcaccataat gaaacatact agagaagatg aaacatttgg ggccaaactg
     1141 tccatattat actagagaaa gattagacat actagaggct gctaacgacg aaaactacgc
     1201 tctggctgct tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg
     1261 cctttcgttt tatctgttgt ttgtcggtga acgctctcta ctagagtcac actggctcac
     1321 cttcgggtgg gcctttctgc gtttata
//