LOCUS       BBa_M45125              1940 bp    DNA     linear       09-Jun-2024
DEFINITION  F-Plasmid OriT with Autolysin for Cell Death Induced Conjugation
ACCESSION   BBa_M45125
VERSION     BBa_M45125.1  
FEATURES             Location/Qualifiers
     oriT            1..365
                     /label=BBa_J01002
     misc_feature    380..386
                     /label=BamHI
     promoter        387..421
                     /label=lacq Promoter
     promoter        374..423
                     /label=BBa_J54200
     RBS             432..446
                     /label=RBS-1\Strong
     RBS             439..443
                     /label=RBS
     misc_feature    432..446
                     /label=BBa_B0030
     RBS             432..446
                     /label=BBa_B0030
     misc_feature    453..455
                     /label=Start Codon
     CDS             455..1871
                     /label=Protein Coding Sequence
     misc_feature    1872..1875
                     /label=Stop Codon
     CDS             453..1940
                     /label=BBa_M45122
ORIGIN
        1 aaggctcaac aggttggtgg ttctcaccac caaaagcacc acaccccacg caaaaacaag
       61 tttttgctga tttttcttta taaatagagt gttatgaaaa attagtttct cttactctct
      121 ttatgatatt taaaaaagcg gtgtcggcgc ggctacaaca acgcgccgac accgttttgt
      181 aggggtggta ctgactattt ttataaaaaa cattatttta tattaggggt gctgctagcg
      241 gcgcggtgtg tttttttata ggataccgct aggggcgctg ctagcggtgc gtccctgttt
      301 gcattatgaa ttttagtgtt tcgaaattaa ctttatttta tgttcaaaaa aggtaatctc
      361 taatgtacta gagccgtgac ggatcctggt gcaaaacctt tcgcggtatg gcatgatagc
      421 gcctactaga gattaaagag gagaaatact agatgcgctc ctacataaaa gttcttacga
      481 tgtgctttct gggtcttatc ttgtttgttc ctaccgctct ggctgataat tctgtgaaaa
      541 gagtgggcgg atcgaatcgc tacggcaccg ctgtgcaaat ctcgaaacag atgtatagca
      601 cagcctctac ggctgttatt gtaggtggta gctcatatgc tgatgcaatc tccgctgctc
      661 cgctggctta ccaaaaaaac gcacccttac tgtatactaa ctcggataaa ctttcatacg
      721 agactaaaac ccgcctgaaa gaaatgcaaa ccaaaaacgt gataattgtc gggggtaccc
      781 cggctgtttc aagcaatacg gcaaatcaaa tcaagtcact gggaatttca ataaaaagga
      841 tcgcaggttc gaatcgttat gacaccgccg cacgggtagc gaaagcaatg ggagcaacaa
      901 gtaaagcagt cattctgaat ggcttcttat atgcagacgc gcccgcagtg atcccttatg
      961 ccgcaaaaaa cggatatccg attcttttca ctaataaaac atccatcaac tctgcgacta
     1021 cttcagtcat taaggataaa gggatatcta gtaccgtggt agtaggtggc acgggtagca
     1081 tatccaatac ggtttacaat aaactaccaa gcccgacaag aatttcgggg agtaaccgtt
     1141 acgagctcgc ggcgaatatt gtacagaaat taaatctgag cacgtccaca gtgtatgtat
     1201 ctaacgggtt tagttaccca gatagtattg caggcgccac tttagccgct aaaaagaagc
     1261 aaagcctgat cttgaccaat ggtgaaaatc tttcgactgg tgcacgcaaa attattggta
     1321 gcaaaaatat gagtaatttt atgatcatcg ggaacactcc ggccgtgagc acaaaagtcg
     1381 ccaaccaact gaagaaccca gttgtgggcg aaacaatttt catcgacccg ggtcatggcg
     1441 accaagatag cggcgcaatt ggcaatggtt tattggaaaa agaagtgaac ctggatatag
     1501 ctaaaagagt aaacacaaaa ctgaatgcct ctggagcctt gcccgtcctg tccagatcga
     1561 acgatacttt ctactctctc caggaacgtg taaacaaagc agcatcagcg caggctgatc
     1621 tgttcctgtc catccatgct aacgccaatg attcatcttc accgaacggc agtgaaactt
     1681 attatgacac tacttatcag gcggctaaca gcaaaagact ggcagagcag attcagccta
     1741 aattagcagc aaacctgggt acacgtgacc gtggtgtgaa aaccgcagcc ttttacgtta
     1801 taaaatattc aaaaatgcca agtgtgcttg tggaaacggc gttcataacc aatgcctcag
     1861 acgcctccaa attaaaacag gcggtctaca aagataaagc tgctcaagca attcacgacg
     1921 ggacggttag ctactatcgt
//