BBa_M45122 1 BBa_M45122 Autolysin N-acetylmuramoyl-L-alanine amidase (cwlB) gene for E coli, complete cds 2014-04-14T11:00:00Z 2015-05-08T01:14:08Z asdfasdf asdfsd false false _1855_ 0 20933 9 Not in stock false asdfasdf false Sherissa Ward annotation2372630 1 Protein Coding Sequence range2372630 1 3 1419 annotation2372583 1 Start Codon range2372583 1 1 3 annotation2372631 1 Stop Codon range2372631 1 1420 1423 BBa_B0030 1 BBa_B0030 RBS.1 (strong) -- modified from R. Weiss 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 Strong RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>. false true _44_46_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix (&quot;orig&quot; in figure 4-14 of Ron Weiss thesis). <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation1701 1 RBS-1\Strong range1701 1 1 15 annotation1702 1 RBS range1702 1 8 12 annotation7025 1 BBa_B0030 range7025 1 1 15 BBa_M45125 1 BBa_M45125 F-Plasmid OriT with Autolysin for Cell Death Induced Conjugation 2014-04-20T11:00:00Z 2015-05-08T01:14:08Z This part was made using an autolysin from Bacillus subtilis and other parts in the registry. OriT is the nic region on the F Plasmid that allows for conjugation in the presence of transfer genes encoded in TraJ genes. This OriT gene is followed by an autolysin N-acetylmuramoyl-L-alanine amidase. This codes for an Autolysin that will cause cell death to the cell upon conjugation. This composite part is meant to be incorporated into a plasmid with the TraJ genes knocked out with a kanamycin resistance gene. This will further prevent conjugation by the bacteria. false false _1855_ 0 20933 9 Not in stock false There may need to be up-regulation of the autolysin in this case to increase the rate of cell death. false Sherissa Ward component2372655 1 BBa_J01002 component2372665 1 BBa_M45122 component2372660 1 BBa_B0030 component2372658 1 BBa_J54200 annotation2372658 1 BBa_J54200 range2372658 1 374 423 annotation2372665 1 BBa_M45122 range2372665 1 453 1940 annotation2372655 1 BBa_J01002 range2372655 1 1 365 annotation2372660 1 BBa_B0030 range2372660 1 432 446 BBa_J01002 1 OriTF OriT-F (Origin of Transfer for the F-plasmid nic region) 2005-10-17T11:00:00Z 2015-08-31T04:08:11Z OriTf (Origin of Transfer for the F-type conjugative plasmid) is the nic region, is where the relaxosome nicks the plasmid and conjugative transfer by F plasmid machinery begins via rolling circle replication. <br> This particular plasmid has been derived from a F-type plasmid donated by Professor Laura Frost University of Alberta. <br> <small> NOTE: OriTf is NOT the same as the host cell's native Origin of Transfer (this is a separate site within the bacterial host's main genome). <br> </small> false false _13_ 0 395 13 It's complicated false true Golden Bear BBa_J54200 1 BBa_J54200 lacq_Promoter 2006-10-14T11:00:00Z 2015-08-31T03:48:12Z DNA synthesis Promoter that express always. false false _76_ 0 762 76 Not in stock false As promoter false Yusaku Nakashima annotation1903214 1 BamHI range1903214 1 7 13 annotation1903215 1 lacq Promoter range1903215 1 14 48 BBa_J54200_sequence 1 ccgtgacggatcctggtgcaaaacctttcgcggtatggcatgatagcgcc BBa_B0030_sequence 1 attaaagaggagaaa BBa_J01002_sequence 1 aaggctcaacaggttggtggttctcaccaccaaaagcaccacaccccacgcaaaaacaagtttttgctgatttttctttataaatagagtgttatgaaaaattagtttctcttactctctttatgatatttaaaaaagcggtgtcggcgcggctacaacaacgcgccgacaccgttttgtaggggtggtactgactatttttataaaaaacattattttatattaggggtgctgctagcggcgcggtgtgtttttttataggataccgctaggggcgctgctagcggtgcgtccctgtttgcattatgaattttagtgtttcgaaattaactttattttatgttcaaaaaaggtaatctctaatg BBa_M45122_sequence 1 atgcgctcctacataaaagttcttacgatgtgctttctgggtcttatcttgtttgttcctaccgctctggctgataattctgtgaaaagagtgggcggatcgaatcgctacggcaccgctgtgcaaatctcgaaacagatgtatagcacagcctctacggctgttattgtaggtggtagctcatatgctgatgcaatctccgctgctccgctggcttaccaaaaaaacgcacccttactgtatactaactcggataaactttcatacgagactaaaacccgcctgaaagaaatgcaaaccaaaaacgtgataattgtcgggggtaccccggctgtttcaagcaatacggcaaatcaaatcaagtcactgggaatttcaataaaaaggatcgcaggttcgaatcgttatgacaccgccgcacgggtagcgaaagcaatgggagcaacaagtaaagcagtcattctgaatggcttcttatatgcagacgcgcccgcagtgatcccttatgccgcaaaaaacggatatccgattcttttcactaataaaacatccatcaactctgcgactacttcagtcattaaggataaagggatatctagtaccgtggtagtaggtggcacgggtagcatatccaatacggtttacaataaactaccaagcccgacaagaatttcggggagtaaccgttacgagctcgcggcgaatattgtacagaaattaaatctgagcacgtccacagtgtatgtatctaacgggtttagttacccagatagtattgcaggcgccactttagccgctaaaaagaagcaaagcctgatcttgaccaatggtgaaaatctttcgactggtgcacgcaaaattattggtagcaaaaatatgagtaattttatgatcatcgggaacactccggccgtgagcacaaaagtcgccaaccaactgaagaacccagttgtgggcgaaacaattttcatcgacccgggtcatggcgaccaagatagcggcgcaattggcaatggtttattggaaaaagaagtgaacctggatatagctaaaagagtaaacacaaaactgaatgcctctggagccttgcccgtcctgtccagatcgaacgatactttctactctctccaggaacgtgtaaacaaagcagcatcagcgcaggctgatctgttcctgtccatccatgctaacgccaatgattcatcttcaccgaacggcagtgaaacttattatgacactacttatcaggcggctaacagcaaaagactggcagagcagattcagcctaaattagcagcaaacctgggtacacgtgaccgtggtgtgaaaaccgcagccttttacgttataaaatattcaaaaatgccaagtgtgcttgtggaaacggcgttcataaccaatgcctcagacgcctccaaattaaaacaggcggtctacaaagataaagctgctcaagcaattcacgacgggacggttagctactatcgt BBa_M45125_sequence 1 aaggctcaacaggttggtggttctcaccaccaaaagcaccacaccccacgcaaaaacaagtttttgctgatttttctttataaatagagtgttatgaaaaattagtttctcttactctctttatgatatttaaaaaagcggtgtcggcgcggctacaacaacgcgccgacaccgttttgtaggggtggtactgactatttttataaaaaacattattttatattaggggtgctgctagcggcgcggtgtgtttttttataggataccgctaggggcgctgctagcggtgcgtccctgtttgcattatgaattttagtgtttcgaaattaactttattttatgttcaaaaaaggtaatctctaatgtactagagccgtgacggatcctggtgcaaaacctttcgcggtatggcatgatagcgcctactagagattaaagaggagaaatactagatgcgctcctacataaaagttcttacgatgtgctttctgggtcttatcttgtttgttcctaccgctctggctgataattctgtgaaaagagtgggcggatcgaatcgctacggcaccgctgtgcaaatctcgaaacagatgtatagcacagcctctacggctgttattgtaggtggtagctcatatgctgatgcaatctccgctgctccgctggcttaccaaaaaaacgcacccttactgtatactaactcggataaactttcatacgagactaaaacccgcctgaaagaaatgcaaaccaaaaacgtgataattgtcgggggtaccccggctgtttcaagcaatacggcaaatcaaatcaagtcactgggaatttcaataaaaaggatcgcaggttcgaatcgttatgacaccgccgcacgggtagcgaaagcaatgggagcaacaagtaaagcagtcattctgaatggcttcttatatgcagacgcgcccgcagtgatcccttatgccgcaaaaaacggatatccgattcttttcactaataaaacatccatcaactctgcgactacttcagtcattaaggataaagggatatctagtaccgtggtagtaggtggcacgggtagcatatccaatacggtttacaataaactaccaagcccgacaagaatttcggggagtaaccgttacgagctcgcggcgaatattgtacagaaattaaatctgagcacgtccacagtgtatgtatctaacgggtttagttacccagatagtattgcaggcgccactttagccgctaaaaagaagcaaagcctgatcttgaccaatggtgaaaatctttcgactggtgcacgcaaaattattggtagcaaaaatatgagtaattttatgatcatcgggaacactccggccgtgagcacaaaagtcgccaaccaactgaagaacccagttgtgggcgaaacaattttcatcgacccgggtcatggcgaccaagatagcggcgcaattggcaatggtttattggaaaaagaagtgaacctggatatagctaaaagagtaaacacaaaactgaatgcctctggagccttgcccgtcctgtccagatcgaacgatactttctactctctccaggaacgtgtaaacaaagcagcatcagcgcaggctgatctgttcctgtccatccatgctaacgccaatgattcatcttcaccgaacggcagtgaaacttattatgacactacttatcaggcggctaacagcaaaagactggcagagcagattcagcctaaattagcagcaaacctgggtacacgtgaccgtggtgtgaaaaccgcagccttttacgttataaaatattcaaaaatgccaagtgtgcttgtggaaacggcgttcataaccaatgcctcagacgcctccaaattaaaacaggcggtctacaaagataaagctgctcaagcaattcacgacgggacggttagctactatcgt igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z