LOCUS       BBa_M45138              1062 bp    DNA     linear       21-May-2024
DEFINITION  Type II Secretion of Chromate and Uranium Reductase with TorA
ACCESSION   BBa_M45138
VERSION     BBa_M45138.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    213..216
                     /label=conserved
     RBS             209..220
                     /label=BBa_B0034
     misc_feature    227..352
                     /label=TorA
     misc_feature    227..229
                     /label=Start
     misc_feature    227..352
                     /label=BBa_K208005
     misc_feature    359..361
                     /label=Start Codon
     CDS             359..925
                     /label=Coding Sequence
     misc_feature    923..925
                     /label=Stop Codon
     CDS             359..925
                     /label=BBa_M45133
     stem_loop       945..988
                     /label=stem_loop
     misc_feature    934..1013
                     /label=BBa_B0010
     terminator      934..1013
                     /label=BBa_B0010
     stem_loop       1029..1048
                     /label=T7 TE
     misc_feature    1055..1055
                     /label=stop
     polyA_site      1049..1062
                     /label=polya
     misc_feature    1022..1062
                     /label=BBa_B0012
     terminator      1022..1062
                     /label=BBa_B0012
     terminator      934..1062
                     /label=BBa_B0015
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatga acaataacga
      241 tctctttcag gcatcacgtc ggcgttttct ggcacaactc ggcggcttaa ccgtcgccgg
      301 gatgctgggg ccgtcattgt taacgccgcg acgtgcgact gcggcgcaag cgtactagat
      361 gtctgaaaaa ttgcaggtgg ttacgttact ggggagcctg cgcaaaggct catttaatgg
      421 catggttgca cgtaccctgc cgaaaattgc tccggcgagc atggaagtca atgcgttacc
      481 atccattgcc gacattccct tgtatgacgc tgacgtacag caggaagaag gttttccagc
      541 aacggttgaa gctctggcgg aacagatccg tcaggctgac ggtgtggtga tcgtcacgcc
      601 ggaatataac tactcggtac cgggtgggct gaaaaatgcc atcgactggc tttcccgcct
      661 gccggatcaa ccgctggccg gtaaaccggt attgattcag accagctcaa tgggcgtgat
      721 tggcggcgcg cgctgtcagg atcacctgcg ccagattctg gttttcctcg atgcaatggt
      781 gatgaacaag ccggaattta tgggcagcgt gattcagacc aaagttgatc cgcaaaccgg
      841 agaagtgatt gatcagggta cgctggacca cctgaccggg caattgaccg catttggtga
      901 gtttattcag cgagttaaga tctaatacta gagccaggca tcaaataaaa cgaaaggctc
      961 agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctctactaga
     1021 gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta ta
//