BBa_B1006
1
BBa_B1006
Terminator (artificial, large, %T~>90)
2006-08-30T11:00:00Z
2015-08-31T04:07:21Z
modified E. coli thr terminator, replaced all A-T pairs in stem with C-G pairs
Released HQ 2013
Artificial terminator, estimated %T~>90%
*8bp stem, 6nt loop
*Bidirectional, estimated reverse %T~>90%
false
true
_41_
0
745
41
In stock
false
Bidirectional, with the reverse estimated to be less effective than the forward. Has a polyA tail of 9 residues.
true
Haiyao Huang
annotation1898430
1
PolyA
range1898430
1
32
39
annotation1898428
1
B1006
range1898428
1
1
39
annotation1898431
1
PolyA
range1898431
1
1
9
annotation1898429
1
modified thr terminator
range1898429
1
10
31
BBa_M45810
1
BBa_M45810
Promoter sequence
2015-04-19T11:00:00Z
2015-05-08T01:14:10Z
Tuc2009 and B. subtilis
Rightward promoter sequence using Tuc2009
false
false
_1855_
0
25679
9
Not in stock
false
Optimized for L. lactis
false
Kaitlyn C. Anderson
component2431266
1
BBa_B0030
component2431264
1
BBa_M45802
component2431271
1
BBa_M45805
component2431276
1
BBa_B1006
annotation2431276
1
BBa_B1006
range2431276
1
516
554
annotation2431264
1
BBa_M45802
range2431264
1
1
97
annotation2431271
1
BBa_M45805
range2431271
1
127
507
annotation2431266
1
BBa_B0030
range2431266
1
106
120
BBa_M45802
1
BBa_M45802
Mutated Tuc2009 bidirectional promoter for decreased CI sensitivity
2015-04-14T11:00:00Z
2015-05-08T01:14:09Z
Kenny JG, Leach S, Hoz AB, Venema G, Kok J, Fitzgerald GF, Nauta S, Alonso JC, Sinderen D (2006) Characterization of the lytic-lysogenic switch of the lactococcal bacteriophage Tuc2009. Virology 347:434-446
A modified bi-directional promoter from lactococcal bacteriophage Tuc2009 containing a bp mutation for decreased binding affinity of the CI repressor protein to the operator OL-CI at high CI concentrations. Allowing for continued up-regulated transcription from the leftward promoter (PL). See BBa_M45801 for detailed description of bi-directional promoter and intergenic switch region regulation with CI and Cro repressor proteins.
false
false
_1855_
0
25667
9
Not in stock
false
G to T change at bp 32 from BBa_M45801 on Fwd strand
false
Thomas Overbeck
annotation2431043
1
OR-CI
range2431043
1
65
92
annotation2431041
1
OR-CRO
range2431041
1
40
78
annotation2431039
1
PR
range2431039
1
64
92
annotation2431037
1
G to T
range2431037
1
32
32
annotation2431042
1
OL-CI
range2431042
1
17
44
annotation2431040
1
OL-CRO
range2431040
1
5
33
annotation2431038
1
PL
range2431038
1
7
36
BBa_B0030
1
BBa_B0030
RBS.1 (strong) -- modified from R. Weiss
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
Strong RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>.
false
true
_44_46_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("orig" in figure 4-14 of Ron Weiss thesis). <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation1702
1
RBS
range1702
1
8
12
annotation1701
1
RBS-1\Strong
range1701
1
1
15
annotation7025
1
BBa_B0030
range7025
1
1
15
BBa_M45805
1
BBa_M45805
Promoter
2015-04-16T11:00:00Z
2015-05-08T01:14:10Z
Originates from B. subtilis
A transcription factor gene that activates P(pspollE)
false
false
_1855_
0
25679
9
Not in stock
false
This sequence has been codon optimized for Lactococcus lactis
false
Kaitlyn C. Anderson
annotation2431106
1
Start Codon
range2431106
1
1
3
annotation2431108
1
Coding Sequence
range2431108
1
4
377
annotation2431107
1
Stop Codon
range2431107
1
378
381
BBa_M45805_sequence
1
atgagcagccagcctgaaccaaagaagaaaaatctcgacgcgagcatcacaagcattatccatgaaatcggcgtcccagcccatattaaaggctatctctatctgcgcgaagcaatctcaatggtatacaatgacatcgaattgctcggcagcattacaaaagtcctctatccggacatcgccaaaaaatttaacacaaccgcaagccgtgtagaaagagcgatccgccatgcaattgaagtggcatggagcagaggaaacattgattccatttcctcgttgtttggttatactgtcagcatgacaaaagctaaacctaccaacagtgaatttattgcaatggttgcggataagctgaggttagagcataaggcttcttaa
BBa_M45810_sequence
1
aaacacattatataagaaaaaaccacgtttttcaaactttttttgtcaaatgacgtttttttcttgacaaaccacgaaaagtggactataattaacttactagagattaaagaggagaaatactagatgagcagccagcctgaaccaaagaagaaaaatctcgacgcgagcatcacaagcattatccatgaaatcggcgtcccagcccatattaaaggctatctctatctgcgcgaagcaatctcaatggtatacaatgacatcgaattgctcggcagcattacaaaagtcctctatccggacatcgccaaaaaatttaacacaaccgcaagccgtgtagaaagagcgatccgccatgcaattgaagtggcatggagcagaggaaacattgattccatttcctcgttgtttggttatactgtcagcatgacaaaagctaaacctaccaacagtgaatttattgcaatggttgcggataagctgaggttagagcataaggcttcttaatactagagaaaaaaaaaccccgcccctgacagggcggggtttttttt
BBa_B0030_sequence
1
attaaagaggagaaa
BBa_M45802_sequence
1
aaacacattatataagaaaaaaccacgtttttcaaactttttttgtcaaatgacgtttttttcttgacaaaccacgaaaagtggactataattaact
BBa_B1006_sequence
1
aaaaaaaaaccccgcccctgacagggcggggtttttttt
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z