BBa_M45901 1 BBa_M45901 Syntaxin-1A 2015-03-31T11:00:00Z 2015-05-08T01:14:10Z Back translation of the amino acid sequence for human Syntaxin-1A optimized for E. coli. Should be able to be cleaved by BoNT/C. ATGAAAGATCGCACTCAGGAACTTAGAACTGCTAAAGATTCGGACGATGACGACGATGTGGCGGTTACGGTTGACCGAGACAGATTTATGGATGAGTTCTTTGAGCAAGTGGAGGAAATTCGAGGGTTTATTGATAAAATCGCGGAAAATGTTGAAGAGGTAAAACGAAAACATAGCGCCATCCTTGCGAGTCCCAATCCGGATGAAAAGACCAAGGAGGAGCTTGAAGAACTAATGTCTGACATTAAGAAAACCGCTAATAAAGTTCGCTCAAAGCTAAAGAGCATTGAGCAGTCCATTGAACAGGAGGAAGGTTTGAATCGGTCGTCAGCAGATCTTCGTATTCGAAAAACACAGCACAGTACACTTAGCCGTAAATTCGTGGAGGTTATGTCGGAATACAATGCGACTCAAAGTGACTATAGGGAACGCTGTAAGGGACGGATTCAGCGTCAGCTCGAAATCACTGGCCGGACAACAACCTCTGAAGAATTAGAAGACATGCTGGAGTCAGGGAATCCGGCTATTTTTGCCTCGGGTATTATCATGGATTCTAGCATATCTAAACAGGCGCTATCCGAAATTGAAACCCGCCACAGTGAAATCATTAAACTAGAGAATAGTATCCGCGAATTGCATGACATGTTCATGGATATGGCCATGCTAGTTGAGTCCCAAGGCGAGATGATTGATCGTATCGAGTATAACGTGGAACATGCAGTCGATTACGTAGAAAGGGCGGTCAGCGACACTAAAAAGGCTGTCAAATATCAGAGCAAAGCGCGCCGGAAGAAAATAATGATTATAATCTGTTGTGTTATTCTGGGCATTGTGATTGCTTCAACCGTTGGGGGCATTTTTGCA false false _1855_ 0 25685 9 Not in stock false Optimized for E. coli false Chad Lawrence Nielsen annotation2430716 1 Coding sequence for Syntaxin-1A range2430716 1 1 864 annotation2430717 1 End of Syntaxin-1A sequence range2430717 1 864 864 annotation2430713 1 Start Codon for Syntaxin-1A range2430713 1 1 3 BBa_M45902 1 BBa_M45902 Botulism Toxin Type C Detector 2015-04-09T11:00:00Z 2015-05-08T01:14:10Z Composite part created from existing parts. This part is designed to detect the presence of botulism toxin type C. It is composed of a ribosome binding site, a fluorescent protein (SBFP2), a cleavage site that the toxin attacks (Syntaxin-1A), and a 10x his-tag. Ideally, this should be able to be purified after production using a nickel or copper column, eluted, and then mixed with the sample. If the toxin is in solution, it will cleave the Syntaxin-1A sequence, separating the fluorescent protein from the his-tag. This means that if the solution is run through a column again, the fluorescent protein should come out during the flow throughs rather than the elutions. false false _1855_ 0 25685 9 Not in stock false Syntaxin-1A is from humans, eukaryotic organisms, so it may not work with E. coli. A blue fluorescent protein was chosen because this part is part of a set for a class project and will be different from GFP and RFP that will be released by different strains of botulism toxin. false Chad Lawrence Nielsen component2430730 1 BBa_B0035 component2430734 1 BBa_M45901 component2430731 1 BBa_K156010 component2430738 1 BBa_K844000 annotation2430730 1 BBa_B0035 range2430730 1 1 14 annotation2430731 1 BBa_K156010 range2430731 1 21 740 annotation2430738 1 BBa_K844000 range2430738 1 1619 1654 annotation2430734 1 BBa_M45901 range2430734 1 747 1610 BBa_B0035 1 BBa_B0035 RBS (B0030 derivative) 2004-01-27T12:00:00Z 2015-08-31T04:07:20Z -- No description -- false false _1_ 0 24 7 Not in stock false false Jason Kelly BBa_K156010 1 SBFP2 SBFP2 (strongly enhanced blue fluorescent protein) 2008-10-28T12:00:00Z 2016-01-26T09:51:52Z OFP was cloned from the tentacles of ''Cerianthus'' sp., a tube anemone. The original sequence was obtained from GenBank (VERSION AY296063.1, GI:31616578). This part codes for orange fluorescent protein (OFP). Fluorescent proteins are useful tools for visualizing and quantifying protein expression in cells. OFP is yet another fluorescent protein, like GFP, that can be utilized as a reporter. false false _246_ 4206 1475 9 It's complicated true This part was codon-optimized for expression in ''E. coli''. The following restriction enzyme sites were also removed: EcoRI, NotI, XbaI, SpeI, PstI, NheI, AvrII, ApoI, MfeI, NsiI, SbfI. false George McArthur IV BBa_K844000 1 BBa_K844000 10x-Histidine (10x-His) Tag with double stop codon (TAATAA) 2012-10-01T11:00:00Z 2015-05-08T01:13:33Z Constructed through oligonucleotide annealing Released HQ 2013 10x-Histidine tag with double stop codon TAATAA to allow for better extraction of tagged products and protein termination in a single part. false false _1104_ 0 9404 9 In stock true none false Kathleen Miller annotation2206609 1 Stop range2206609 1 34 36 annotation2206608 1 Stop range2206608 1 31 33 annotation2206607 1 10x-Histidine Tag range2206607 1 1 30 BBa_B0035_sequence 1 attaaagaggagaa BBa_M45901_sequence 1 atgaaagatcgcactcaggaacttagaactgctaaagattcggacgatgacgacgatgtggcggttacggttgaccgagacagatttatggatgagttctttgagcaagtggaggaaattcgagggtttattgataaaatcgcggaaaatgttgaagaggtaaaacgaaaacatagcgccatccttgcgagtcccaatccggatgaaaagaccaaggaggagcttgaagaactaatgtctgacattaagaaaaccgctaataaagttcgctcaaagctaaagagcattgagcagtccattgaacaggaggaaggtttgaatcggtcgtcagcagatcttcgtattcgaaaaacacagcacagtacacttagccgtaaattcgtggaggttatgtcggaatacaatgcgactcaaagtgactatagggaacgctgtaagggacggattcagcgtcagctcgaaatcactggccggacaacaacctctgaagaattagaagacatgctggagtcagggaatccggctatttttgcctcgggtattatcatggattctagcatatctaaacaggcgctatccgaaattgaaacccgccacagtgaaatcattaaactagagaatagtatccgcgaattgcatgacatgttcatggatatggccatgctagttgagtcccaaggcgagatgattgatcgtatcgagtataacgtggaacatgcagtcgattacgtagaaagggcggtcagcgacactaaaaaggctgtcaaatatcagagcaaagcgcgccggaagaaaataatgattataatctgttgtgttattctgggcattgtgattgcttcaaccgttgggggcatttttgca BBa_K844000_sequence 1 catcatcaccatcaccaccatcatcaccattaataa BBa_K156010_sequence 1 atggttagcaaaggtgaagagctgtttactggtgttgttccgatcctggttgaactggacggtgatgttaacggccacaagttctccgtgtctggcgaaggcgagggcgatgctacctacggtaaactgaccctgaagttcatctgtaccactggtaaactgccggtaccgtggccgaccctggttaccaccctgagccatggtgttcagtgttttgcacgttacccggatcacatgaagcagcatgatttcttcaagtctgcgatgccggagggttacgtccaggaacgcactatcttcttcaaagacgacggcaactataaaacccgtgccgaggttaagtttgagggtgacaccctggtcaaccgtattgaactgaaaggtatcgacttcaaagaagacggcaacattctgggtcacaaactggagtacaacttcaacagccataacgtgtacatcaccgccgataaacagaagaacggcatcaaagctaacttcaagattcgtcacaacatcgaagatggtggtgttcaactggctgatcactaccagcagaacacgccgatcggtgatggcccggtactgctgcctgacaaccattatctgtctacccagtctaaactgagcaaagacccgaatgagaaacgtgatcacatggtgctgctggagttcgtgactgcggcaggtatcacgctgggtatggacgaactgtataaatga BBa_M45902_sequence 1 attaaagaggagaatactagatggttagcaaaggtgaagagctgtttactggtgttgttccgatcctggttgaactggacggtgatgttaacggccacaagttctccgtgtctggcgaaggcgagggcgatgctacctacggtaaactgaccctgaagttcatctgtaccactggtaaactgccggtaccgtggccgaccctggttaccaccctgagccatggtgttcagtgttttgcacgttacccggatcacatgaagcagcatgatttcttcaagtctgcgatgccggagggttacgtccaggaacgcactatcttcttcaaagacgacggcaactataaaacccgtgccgaggttaagtttgagggtgacaccctggtcaaccgtattgaactgaaaggtatcgacttcaaagaagacggcaacattctgggtcacaaactggagtacaacttcaacagccataacgtgtacatcaccgccgataaacagaagaacggcatcaaagctaacttcaagattcgtcacaacatcgaagatggtggtgttcaactggctgatcactaccagcagaacacgccgatcggtgatggcccggtactgctgcctgacaaccattatctgtctacccagtctaaactgagcaaagacccgaatgagaaacgtgatcacatggtgctgctggagttcgtgactgcggcaggtatcacgctgggtatggacgaactgtataaatgatactagatgaaagatcgcactcaggaacttagaactgctaaagattcggacgatgacgacgatgtggcggttacggttgaccgagacagatttatggatgagttctttgagcaagtggaggaaattcgagggtttattgataaaatcgcggaaaatgttgaagaggtaaaacgaaaacatagcgccatccttgcgagtcccaatccggatgaaaagaccaaggaggagcttgaagaactaatgtctgacattaagaaaaccgctaataaagttcgctcaaagctaaagagcattgagcagtccattgaacaggaggaaggtttgaatcggtcgtcagcagatcttcgtattcgaaaaacacagcacagtacacttagccgtaaattcgtggaggttatgtcggaatacaatgcgactcaaagtgactatagggaacgctgtaagggacggattcagcgtcagctcgaaatcactggccggacaacaacctctgaagaattagaagacatgctggagtcagggaatccggctatttttgcctcgggtattatcatggattctagcatatctaaacaggcgctatccgaaattgaaacccgccacagtgaaatcattaaactagagaatagtatccgcgaattgcatgacatgttcatggatatggccatgctagttgagtcccaaggcgagatgattgatcgtatcgagtataacgtggaacatgcagtcgattacgtagaaagggcggtcagcgacactaaaaaggctgtcaaatatcagagcaaagcgcgccggaagaaaataatgattataatctgttgtgttattctgggcattgtgattgcttcaaccgttgggggcatttttgcatactagagcatcatcaccatcaccaccatcatcaccattaataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z