BBa_M45901
1
BBa_M45901
Syntaxin-1A
2015-03-31T11:00:00Z
2015-05-08T01:14:10Z
Back translation of the amino acid sequence for human Syntaxin-1A optimized for E. coli. Should be able to be cleaved by BoNT/C.
ATGAAAGATCGCACTCAGGAACTTAGAACTGCTAAAGATTCGGACGATGACGACGATGTGGCGGTTACGGTTGACCGAGACAGATTTATGGATGAGTTCTTTGAGCAAGTGGAGGAAATTCGAGGGTTTATTGATAAAATCGCGGAAAATGTTGAAGAGGTAAAACGAAAACATAGCGCCATCCTTGCGAGTCCCAATCCGGATGAAAAGACCAAGGAGGAGCTTGAAGAACTAATGTCTGACATTAAGAAAACCGCTAATAAAGTTCGCTCAAAGCTAAAGAGCATTGAGCAGTCCATTGAACAGGAGGAAGGTTTGAATCGGTCGTCAGCAGATCTTCGTATTCGAAAAACACAGCACAGTACACTTAGCCGTAAATTCGTGGAGGTTATGTCGGAATACAATGCGACTCAAAGTGACTATAGGGAACGCTGTAAGGGACGGATTCAGCGTCAGCTCGAAATCACTGGCCGGACAACAACCTCTGAAGAATTAGAAGACATGCTGGAGTCAGGGAATCCGGCTATTTTTGCCTCGGGTATTATCATGGATTCTAGCATATCTAAACAGGCGCTATCCGAAATTGAAACCCGCCACAGTGAAATCATTAAACTAGAGAATAGTATCCGCGAATTGCATGACATGTTCATGGATATGGCCATGCTAGTTGAGTCCCAAGGCGAGATGATTGATCGTATCGAGTATAACGTGGAACATGCAGTCGATTACGTAGAAAGGGCGGTCAGCGACACTAAAAAGGCTGTCAAATATCAGAGCAAAGCGCGCCGGAAGAAAATAATGATTATAATCTGTTGTGTTATTCTGGGCATTGTGATTGCTTCAACCGTTGGGGGCATTTTTGCA
false
false
_1855_
0
25685
9
Not in stock
false
Optimized for E. coli
false
Chad Lawrence Nielsen
annotation2430716
1
Coding sequence for Syntaxin-1A
range2430716
1
1
864
annotation2430717
1
End of Syntaxin-1A sequence
range2430717
1
864
864
annotation2430713
1
Start Codon for Syntaxin-1A
range2430713
1
1
3
BBa_M45902
1
BBa_M45902
Botulism Toxin Type C Detector
2015-04-09T11:00:00Z
2015-05-08T01:14:10Z
Composite part created from existing parts.
This part is designed to detect the presence of botulism toxin type C. It is composed of a ribosome binding site, a fluorescent protein (SBFP2), a cleavage site that the toxin attacks (Syntaxin-1A), and a 10x his-tag. Ideally, this should be able to be purified after production using a nickel or copper column, eluted, and then mixed with the sample. If the toxin is in solution, it will cleave the Syntaxin-1A sequence, separating the fluorescent protein from the his-tag. This means that if the solution is run through a column again, the fluorescent protein should come out during the flow throughs rather than the elutions.
false
false
_1855_
0
25685
9
Not in stock
false
Syntaxin-1A is from humans, eukaryotic organisms, so it may not work with E. coli. A blue fluorescent protein was chosen because this part is part of a set for a class project and will be different from GFP and RFP that will be released by different strains of botulism toxin.
false
Chad Lawrence Nielsen
component2430730
1
BBa_B0035
component2430734
1
BBa_M45901
component2430731
1
BBa_K156010
component2430738
1
BBa_K844000
annotation2430730
1
BBa_B0035
range2430730
1
1
14
annotation2430731
1
BBa_K156010
range2430731
1
21
740
annotation2430738
1
BBa_K844000
range2430738
1
1619
1654
annotation2430734
1
BBa_M45901
range2430734
1
747
1610
BBa_B0035
1
BBa_B0035
RBS (B0030 derivative)
2004-01-27T12:00:00Z
2015-08-31T04:07:20Z
-- No description --
false
false
_1_
0
24
7
Not in stock
false
false
Jason Kelly
BBa_K156010
1
SBFP2
SBFP2 (strongly enhanced blue fluorescent protein)
2008-10-28T12:00:00Z
2016-01-26T09:51:52Z
OFP was cloned from the tentacles of ''Cerianthus'' sp., a tube anemone. The original sequence was obtained from GenBank (VERSION AY296063.1, GI:31616578).
This part codes for orange fluorescent protein (OFP). Fluorescent proteins are useful tools for visualizing and quantifying protein expression in cells. OFP is yet another fluorescent protein, like GFP, that can be utilized as a reporter.
false
false
_246_
4206
1475
9
It's complicated
true
This part was codon-optimized for expression in ''E. coli''. The following restriction enzyme sites were also removed: EcoRI, NotI, XbaI, SpeI, PstI, NheI, AvrII, ApoI, MfeI, NsiI, SbfI.
false
George McArthur IV
BBa_K844000
1
BBa_K844000
10x-Histidine (10x-His) Tag with double stop codon (TAATAA)
2012-10-01T11:00:00Z
2015-05-08T01:13:33Z
Constructed through oligonucleotide annealing
Released HQ 2013
10x-Histidine tag with double stop codon TAATAA to allow for better extraction of tagged products and protein termination in a single part.
false
false
_1104_
0
9404
9
In stock
true
none
false
Kathleen Miller
annotation2206609
1
Stop
range2206609
1
34
36
annotation2206608
1
Stop
range2206608
1
31
33
annotation2206607
1
10x-Histidine Tag
range2206607
1
1
30
BBa_B0035_sequence
1
attaaagaggagaa
BBa_M45901_sequence
1
atgaaagatcgcactcaggaacttagaactgctaaagattcggacgatgacgacgatgtggcggttacggttgaccgagacagatttatggatgagttctttgagcaagtggaggaaattcgagggtttattgataaaatcgcggaaaatgttgaagaggtaaaacgaaaacatagcgccatccttgcgagtcccaatccggatgaaaagaccaaggaggagcttgaagaactaatgtctgacattaagaaaaccgctaataaagttcgctcaaagctaaagagcattgagcagtccattgaacaggaggaaggtttgaatcggtcgtcagcagatcttcgtattcgaaaaacacagcacagtacacttagccgtaaattcgtggaggttatgtcggaatacaatgcgactcaaagtgactatagggaacgctgtaagggacggattcagcgtcagctcgaaatcactggccggacaacaacctctgaagaattagaagacatgctggagtcagggaatccggctatttttgcctcgggtattatcatggattctagcatatctaaacaggcgctatccgaaattgaaacccgccacagtgaaatcattaaactagagaatagtatccgcgaattgcatgacatgttcatggatatggccatgctagttgagtcccaaggcgagatgattgatcgtatcgagtataacgtggaacatgcagtcgattacgtagaaagggcggtcagcgacactaaaaaggctgtcaaatatcagagcaaagcgcgccggaagaaaataatgattataatctgttgtgttattctgggcattgtgattgcttcaaccgttgggggcatttttgca
BBa_K844000_sequence
1
catcatcaccatcaccaccatcatcaccattaataa
BBa_K156010_sequence
1
atggttagcaaaggtgaagagctgtttactggtgttgttccgatcctggttgaactggacggtgatgttaacggccacaagttctccgtgtctggcgaaggcgagggcgatgctacctacggtaaactgaccctgaagttcatctgtaccactggtaaactgccggtaccgtggccgaccctggttaccaccctgagccatggtgttcagtgttttgcacgttacccggatcacatgaagcagcatgatttcttcaagtctgcgatgccggagggttacgtccaggaacgcactatcttcttcaaagacgacggcaactataaaacccgtgccgaggttaagtttgagggtgacaccctggtcaaccgtattgaactgaaaggtatcgacttcaaagaagacggcaacattctgggtcacaaactggagtacaacttcaacagccataacgtgtacatcaccgccgataaacagaagaacggcatcaaagctaacttcaagattcgtcacaacatcgaagatggtggtgttcaactggctgatcactaccagcagaacacgccgatcggtgatggcccggtactgctgcctgacaaccattatctgtctacccagtctaaactgagcaaagacccgaatgagaaacgtgatcacatggtgctgctggagttcgtgactgcggcaggtatcacgctgggtatggacgaactgtataaatga
BBa_M45902_sequence
1
attaaagaggagaatactagatggttagcaaaggtgaagagctgtttactggtgttgttccgatcctggttgaactggacggtgatgttaacggccacaagttctccgtgtctggcgaaggcgagggcgatgctacctacggtaaactgaccctgaagttcatctgtaccactggtaaactgccggtaccgtggccgaccctggttaccaccctgagccatggtgttcagtgttttgcacgttacccggatcacatgaagcagcatgatttcttcaagtctgcgatgccggagggttacgtccaggaacgcactatcttcttcaaagacgacggcaactataaaacccgtgccgaggttaagtttgagggtgacaccctggtcaaccgtattgaactgaaaggtatcgacttcaaagaagacggcaacattctgggtcacaaactggagtacaacttcaacagccataacgtgtacatcaccgccgataaacagaagaacggcatcaaagctaacttcaagattcgtcacaacatcgaagatggtggtgttcaactggctgatcactaccagcagaacacgccgatcggtgatggcccggtactgctgcctgacaaccattatctgtctacccagtctaaactgagcaaagacccgaatgagaaacgtgatcacatggtgctgctggagttcgtgactgcggcaggtatcacgctgggtatggacgaactgtataaatgatactagatgaaagatcgcactcaggaacttagaactgctaaagattcggacgatgacgacgatgtggcggttacggttgaccgagacagatttatggatgagttctttgagcaagtggaggaaattcgagggtttattgataaaatcgcggaaaatgttgaagaggtaaaacgaaaacatagcgccatccttgcgagtcccaatccggatgaaaagaccaaggaggagcttgaagaactaatgtctgacattaagaaaaccgctaataaagttcgctcaaagctaaagagcattgagcagtccattgaacaggaggaaggtttgaatcggtcgtcagcagatcttcgtattcgaaaaacacagcacagtacacttagccgtaaattcgtggaggttatgtcggaatacaatgcgactcaaagtgactatagggaacgctgtaagggacggattcagcgtcagctcgaaatcactggccggacaacaacctctgaagaattagaagacatgctggagtcagggaatccggctatttttgcctcgggtattatcatggattctagcatatctaaacaggcgctatccgaaattgaaacccgccacagtgaaatcattaaactagagaatagtatccgcgaattgcatgacatgttcatggatatggccatgctagttgagtcccaaggcgagatgattgatcgtatcgagtataacgtggaacatgcagtcgattacgtagaaagggcggtcagcgacactaaaaaggctgtcaaatatcagagcaaagcgcgccggaagaaaataatgattataatctgttgtgttattctgggcattgtgattgcttcaaccgttgggggcatttttgcatactagagcatcatcaccatcaccaccatcatcaccattaataa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z