LOCUS       BBa_M45905               961 bp    DNA     linear       25-May-2024
DEFINITION  Composite part for fluorescent detection of botulinum neurotoxin A 
            (BoNT A)
ACCESSION   BBa_M45905
VERSION     BBa_M45905.1  
FEATURES             Location/Qualifiers
     RBS             1..14
                     /label=BBa_B0035
     CDS             21..740
                     /label=GFP protein
     CDS             21..740
                     /label=BBa_E0040
     misc_feature    917..917
                     /label=End of sequence
     misc_feature    747..749
                     /label=Start codon
     misc_feature    758..758
                     /label=Point mutation to make it RFC compatible
     CDS             747..917
                     /label=BBa_M45904
     misc_feature    926..955
                     /label=10x-Histidine Tag
     misc_feature    956..958
                     /label=Stop
     misc_feature    959..961
                     /label=Stop
     misc_feature    926..961
                     /label=BBa_K844000
ORIGIN
        1 attaaagagg agaatactag atgcgtaaag gagaagaact tttcactgga gttgtcccaa
       61 ttcttgttga attagatggt gatgttaatg ggcacaaatt ttctgtcagt ggagagggtg
      121 aaggtgatgc aacatacgga aaacttaccc ttaaatttat ttgcactact ggaaaactac
      181 ctgttccatg gccaacactt gtcactactt tcggttatgg tgttcaatgc tttgcgagat
      241 acccagatca tatgaaacag catgactttt tcaagagtgc catgcccgaa ggttatgtac
      301 aggaaagaac tatatttttc aaagatgacg ggaactacaa gacacgtgct gaagtcaagt
      361 ttgaaggtga tacccttgtt aatagaatcg agttaaaagg tattgatttt aaagaagatg
      421 gaaacattct tggacacaaa ttggaataca actataactc acacaatgta tacatcatgg
      481 cagacaaaca aaagaatgga atcaaagtta acttcaaaat tagacacaac attgaagatg
      541 gaagcgttca actagcagac cattatcaac aaaatactcc aattggcgat ggccctgtcc
      601 ttttaccaga caaccattac ctgtccacac aatctgccct ttcgaaagat cccaacgaaa
      661 agagagacca catggtcctt cttgagtttg taacagctgc tgggattaca catggcatgg
      721 atgaactata caaataataa tactagatgg acgagaacct agaacaagta agcgggatca
      781 ttggcaatct gcgccatatg gcactagata tgggtaacga gattgacact cagaaccgcc
      841 aaattgatcg aataatggaa aaagcagatt ccaacaaaac gcgtattgac gaagcgaatc
      901 agcgcgctac aaagatgtac tagagcatca tcaccatcac caccatcatc accattaata
      961 a
//