LOCUS       BBa_M50051              1226 bp    DNA     linear       01-Jun-2024
DEFINITION  IPTG Inducible Plasmid for the Expression of LsrR Repressor
ACCESSION   BBa_M50051
VERSION     BBa_M50051.1  
FEATURES             Location/Qualifiers
     promoter        1..126
                     /label=T5-lac promoter
     misc_feature    1..26
                     /label=lacO1 site
     misc_feature    69..86
                     /label=lacO1 site
     misc_feature    94..123
                     /label=lacOi site
     promoter        63..68
                     /label=-35 signal
     promoter        87..93
                     /label=-10 signal
     promoter        1..126
                     /label=BBa_K592008
     RBS             135..146
                     /label=BBa_J61100
     promoter        153..1109
                     /label=BBa_M50028
     misc_feature    1118..1138
                     /label=BBa_K133133
     stem_loop       1158..1201
                     /label=stem_loop
     misc_feature    1147..1226
                     /label=BBa_B0010
     terminator      1147..1226
                     /label=BBa_B0010
ORIGIN
        1 tgtggaattg tgagcggata acaattacga gcttcatgca cagtgaaatc atgaaaaatt
       61 tatttgcttt gtgagcggat aacaattata atatgtggaa ttgtgagcgc tcacaattcc
      121 acaacgtact agagaaagag gggacatact agatgacaat caacgattcg gcaatttcag
      181 aacagggaat gtgtgaagaa gaacaggtcg cgcggatcgc gtggttttac tatcacgacg
      241 ggctgaccca gagcgagatc agcgatcgtc tcggcctgac acgtttgaaa gtgtcgcgat
      301 tgctggagaa agggcatcag tccggcatta ttcgcgtaca gattaattct cgctttgaag
      361 gctgtctgga atatgaaact caattacgtc gtcagttttc gctgcaacat gtccgggtga
      421 tccctgggct tgcggatgct gatgtcggtg ggcgactggg gataggcgcg gcgcatatgt
      481 tgatgagttt acttcaacca caacagatgc tggcgattgg ttttggcgag gcaaccatga
      541 atacgctgca acgcttaagt ggttttattt cgtcacagca aattcgcctg gtcacgctct
      601 ccggtggcgt cggttcttat atgacgggaa tcgggcagct taacgcggcg tgcagtgtga
      661 atattattcc ggctccgttg cgggcatcct ccgctgacat tgcccgtacg ctaaaaaatg
      721 aaaattgcgt caaagatgtt ctgttagccg cgcaagcagc ggatgtggcg attgtcggca
      781 ttggtgctgt gagtcaacag gacgatgcga caatcattcg ctccggatat attagccagg
      841 gcgaacagtt aatgattggc cgaaaagggg cggttggcga cattttaggc tacttttttg
      901 atgcaaaagg tgacgttgtc acgaatatca aaatacataa cgaactgatt ggcttacctt
      961 taagcgcgct gaagaccata cccgtccggg ttggcgtggc agggggagaa aataaagccg
     1021 aagcaattgc cgctgcaatg aaaggcggtt atatcaacgc actggttacc gatcaggaca
     1081 cagcagcggc gattttacgt agttaatagt actagagcac caccaccacc accactagta
     1141 ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta
     1201 tctgttgttt gtcggtgaac gctctc
//