LOCUS       BBa_P0474                603 bp    DNA     linear       08-May-2024
DEFINITION  Pen1 protein generator
ACCESSION   BBa_P0474
VERSION     BBa_P0474.1  
FEATURES             Location/Qualifiers
     misc_feature    5..8
                     /label=conserved
     RBS             1..12
                     /label=BBa_B0034
     CDS             19..405
                     /label=penI
     misc_feature    403..435
                     /label=LVA
     misc_feature    442..466
                     /label=Help:Barcodes
     CDS             19..441
                     /label=BBa_C0074
     stem_loop       486..529
                     /label=stem_loop
     misc_feature    475..554
                     /label=BBa_B0010
     terminator      475..554
                     /label=BBa_B0010
     stem_loop       570..589
                     /label=T7 TE
     misc_feature    596..596
                     /label=stop
     polyA_site      590..603
                     /label=polya
     misc_feature    563..603
                     /label=BBa_B0012
     terminator      563..603
                     /label=BBa_B0012
ORIGIN
        1 aaagaggaga aatactagat gaaaaaaata ccacagattt ctgatgcgga actcgaagtc
       61 atgaaagtga tttggaagca ttcttccatt aatactaatg aggtaatcaa agagctcagt
      121 aaaacttcaa cgtggagccc aaaaactatt cagactatgc tgctgcgcct tatcaaaaaa
      181 ggcgctctca accaccataa agaaggtcgg gttttcgttt acacgcctaa tatagacgaa
      241 tcagattata tagaggttaa gtcacactca tttctcaacc ggttttacaa tggtacatta
      301 aattccatgg tactcaactt tttggagaat gatcaactgt cgggagaaga aatcaatgaa
      361 ttgtatcaga tactcgaaga acataagaac cgtaagaagg aagctgcaaa cgacgaaaac
      421 tacgctttag tagcttaata actctgatag tgctagtgta gatctctact agagccaggc
      481 atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt
      541 cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct ttctgcgttt
      601 ata
//