ccdB

BBa_P1016 Version 1

Component

This part has been discontinued.

Source:
http://parts.igem.org/Part:BBa_P1016
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Reshma Shetty
Date created: 2007-01-29 12:00:00
Date modified: 2015-06-15 01:13:47

ccdB cassette without ccdA-



    • Details

    Types
    DnaRegion

    Roles
    Generator

    engineered_region

    Sequences BBa_P1016_sequence (Version 1)

    Description

    This part is a differs from BBa_P1011 in that the mutations introduced in BBa_P1011 to remove restriction sites are not present in this part. It does have the "normal" ccd promoter and ccdB coding region. It also has a double TAA stop codon as per BioBrick conventions.

    Notes

    ccdA- was eliminated since it is an inactive form of ccdA and should not be required for the toxic phenotype of ccdB.

    Source

    This part is derived from BBa_P1010 but it lacks ccdA-.

    Sequence Annotation Location Component / Role(s)
    -35
    -10
    ccdB
    		41,46
    64,69
    108,416
    		feature/promoter promoter
    feature/promoter promoter
    feature/cds CDS

    igem#experience
    Works
     
    igem#sampleStatus
    Not in stock
    igem#status
    Deleted
     
    synbiohub#ownedBy
    user/james
     
    synbiohub#ownedBy
    user/myers
     
    synbiohub#topLevel
    BBa_P1016/1
     

    Attachments

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