LOCUS       BBa_P121103             2394 bp    DNA     linear       10-Jun-2024
DEFINITION  JMT Expression Cassette (F2620, PCR)
ACCESSION   BBa_P121103
VERSION     BBa_P121103.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    67..70
                     /label=conserved
     RBS             63..74
                     /label=BBa_B0034
     misc_feature    81..82
                     /label=prefix
     misc_feature    254..254
                     /label=T
     misc_feature    572..572
                     /label=A
     CDS             81..830
                     /label=luxR
     misc_feature    81..836
                     /label=BBa_C0062
     misc_feature    837..861
                     /label=Help:Barcodes
     CDS             81..836
                     /label=BBa_C0062
     stem_loop       881..924
                     /label=stem_loop
     misc_feature    870..949
                     /label=BBa_B0010
     terminator      870..949
                     /label=BBa_B0010
     stem_loop       965..984
                     /label=T7 TE
     misc_feature    991..991
                     /label=stop
     polyA_site      985..998
                     /label=polya
     misc_feature    958..998
                     /label=BBa_B0012
     terminator      958..998
                     /label=BBa_B0012
     terminator      870..998
                     /label=BBa_B0015
     misc_feature    1007..1026
                     /label=LuxR/HSL
     promoter        1026..1031
                     /label=-35
     promoter        1048..1053
                     /label=-10
     misc_feature    1059..1059
                     /label=start
     misc_feature    1007..1061
                     /label=BBa_R0062
     promoter        1007..1061
                     /label=BBa_R0062
     misc_feature    1..1061
                     /label=BBa_F2620
     misc_feature    1074..1077
                     /label=conserved
     RBS             1070..1081
                     /label=BBa_B0034
     CDS             1088..2257
                     /label=BBa_C100003
     stem_loop       2277..2320
                     /label=stem_loop
     misc_feature    2266..2345
                     /label=BBa_B0010
     terminator      2266..2345
                     /label=BBa_B0010
     stem_loop       2361..2380
                     /label=T7 TE
     misc_feature    2387..2387
                     /label=stop
     polyA_site      2381..2394
                     /label=polya
     misc_feature    2354..2394
                     /label=BBa_B0012
     terminator      2354..2394
                     /label=BBa_B0012
     misc_feature    1070..2394
                     /label=BBa_P101103
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaaagagga gaaatactag atgaaaaaca taaatgccga cgacacatac agaataatta
      121 ataaaattaa agcttgtaga agcaataatg atattaatca atgcttatct gatatgacta
      181 aaatggtaca ttgtgaatat tatttactcg cgatcattta tcctcattct atggttaaat
      241 ctgatatttc aatcctagat aattacccta aaaaatggag gcaatattat gatgacgcta
      301 atttaataaa atatgatcct atagtagatt attctaactc caatcattca ccaattaatt
      361 ggaatatatt tgaaaacaat gctgtaaata aaaaatctcc aaatgtaatt aaagaagcga
      421 aaacatcagg tcttatcact gggtttagtt tccctattca tacggctaac aatggcttcg
      481 gaatgcttag ttttgcacat tcagaaaaag acaactatat agatagttta tttttacatg
      541 cgtgtatgaa cataccatta attgttcctt ctctagttga taattatcga aaaataaata
      601 tagcaaataa taaatcaaac aacgatttaa ccaaaagaga aaaagaatgt ttagcgtggg
      661 catgcgaagg aaaaagctct tgggatattt caaaaatatt aggttgcagt gagcgtactg
      721 tcactttcca tttaaccaat gcgcaaatga aactcaatac aacaaaccgc tgccaaagta
      781 tttctaaagc aattttaaca ggagcaattg attgcccata ctttaaaaat taataacact
      841 gatagtgcta gtgtagatca ctactagagc caggcatcaa ataaaacgaa aggctcagtc
      901 gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca
      961 cactggctca ccttcgggtg ggcctttctg cgtttatata ctagagacct gtaggatcgt
     1021 acaggtttac gcaagaaaat ggtttgttat agtcgaataa atactagaga aagaggagaa
     1081 atactagatg gaggtaatgc gagttcttca catgaacaaa ggaaacgggg aaacaagtta
     1141 tgccaagaac tccaccgctc agagcaacat aatatctcta ggcagaagag taatggacga
     1201 ggccttgaag aagttaatga tgagcaattc agagatttcg agcattggaa tcgccgactt
     1261 aggctgctcc tccggtccga acagtctctt gtccatctcc aacatagttg acacgatcca
     1321 caacttgtgt cctgacctcg accgtccagt ccctgagctc agagtctctc tcaacgacct
     1381 ccctagcaat gacttcaact acatatgtgc ttctttgcca gagttttacg accgggttaa
     1441 taataacaag gagggtttag ggttcggtcg tggaggagga gaatcgtgtt ttgtgtcggc
     1501 cgtcccaggt tcgttctacg gacgtttgtt tcctcgccgg agccttcact ttgtgcattc
     1561 ttcttctagt ttacattggt tgtctcaggt tccatgtcgt gaggcggaga aggaagacag
     1621 gacaataaca gctgatttag aaaacatggg gaaaatatac atatcaaaga caagtcctaa
     1681 gagtgcacat aaagcttatg ctcttcaatt ccaaactgat ttcttggttt ttttgaggtc
     1741 acgatctgag gagttggtcc cgggaggccg aatggtttta tcgttccttg gtagaagatc
     1801 actggatccc acaaccgaag agagttgcta tcaatgggaa ctcctagctc aagctcttat
     1861 gtccatggcc aaagagggta tcatcgagga agagaagatc gatgctttca acgctcctta
     1921 ctatgctgcg agctccgaag agttgaaaat ggtgatagag aaagaagggt cattttcgat
     1981 cgataggctt gagataagtc cgattgattg ggaaggtggg agtatcagtg aggagagtta
     2041 tgaccttgca ataaggtcca aacccgaagc cctagctagt ggccgaagag tgtctaatac
     2101 cataagagct gtggtcgagc cgatgctaga acctactttc ggtgaaaatg tgatggacga
     2161 gctttttgaa aggtatgcaa agatcgtggg agagtacttc tatgtaagct cgccacgata
     2221 cgctattgtt attctttcgc tcgttagaac cggttgatac tagagccagg catcaaataa
     2281 aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg
     2341 ctctctacta gagtcacact ggctcacctt cgggtgggcc tttctgcgtt tata
//