LOCUS       BBa_P121109             3024 bp    DNA     linear       10-Jun-2024
DEFINITION  LMS Expression Cassette (F2620)
ACCESSION   BBa_P121109
VERSION     BBa_P121109.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    67..70
                     /label=conserved
     RBS             63..74
                     /label=BBa_B0034
     misc_feature    81..82
                     /label=prefix
     misc_feature    254..254
                     /label=T
     misc_feature    572..572
                     /label=A
     CDS             81..830
                     /label=luxR
     misc_feature    81..836
                     /label=BBa_C0062
     misc_feature    837..861
                     /label=Help:Barcodes
     CDS             81..836
                     /label=BBa_C0062
     stem_loop       881..924
                     /label=stem_loop
     misc_feature    870..949
                     /label=BBa_B0010
     terminator      870..949
                     /label=BBa_B0010
     stem_loop       965..984
                     /label=T7 TE
     misc_feature    991..991
                     /label=stop
     polyA_site      985..998
                     /label=polya
     misc_feature    958..998
                     /label=BBa_B0012
     terminator      958..998
                     /label=BBa_B0012
     terminator      870..998
                     /label=BBa_B0015
     misc_feature    1007..1026
                     /label=LuxR/HSL
     promoter        1026..1031
                     /label=-35
     promoter        1048..1053
                     /label=-10
     misc_feature    1059..1059
                     /label=start
     misc_feature    1007..1061
                     /label=BBa_R0062
     promoter        1007..1061
                     /label=BBa_R0062
     misc_feature    1..1061
                     /label=BBa_F2620
     misc_feature    1074..1077
                     /label=conserved
     RBS             1070..1081
                     /label=BBa_B0034
     CDS             1088..2887
                     /label=BBa_C100009
     stem_loop       2907..2950
                     /label=stem_loop
     misc_feature    2896..2975
                     /label=BBa_B0010
     terminator      2896..2975
                     /label=BBa_B0010
     stem_loop       2991..3010
                     /label=T7 TE
     misc_feature    3017..3017
                     /label=stop
     polyA_site      3011..3024
                     /label=polya
     misc_feature    2984..3024
                     /label=BBa_B0012
     terminator      2984..3024
                     /label=BBa_B0012
     misc_feature    1070..3024
                     /label=BBa_P101109
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaaagagga gaaatactag atgaaaaaca taaatgccga cgacacatac agaataatta
      121 ataaaattaa agcttgtaga agcaataatg atattaatca atgcttatct gatatgacta
      181 aaatggtaca ttgtgaatat tatttactcg cgatcattta tcctcattct atggttaaat
      241 ctgatatttc aatcctagat aattacccta aaaaatggag gcaatattat gatgacgcta
      301 atttaataaa atatgatcct atagtagatt attctaactc caatcattca ccaattaatt
      361 ggaatatatt tgaaaacaat gctgtaaata aaaaatctcc aaatgtaatt aaagaagcga
      421 aaacatcagg tcttatcact gggtttagtt tccctattca tacggctaac aatggcttcg
      481 gaatgcttag ttttgcacat tcagaaaaag acaactatat agatagttta tttttacatg
      541 cgtgtatgaa cataccatta attgttcctt ctctagttga taattatcga aaaataaata
      601 tagcaaataa taaatcaaac aacgatttaa ccaaaagaga aaaagaatgt ttagcgtggg
      661 catgcgaagg aaaaagctct tgggatattt caaaaatatt aggttgcagt gagcgtactg
      721 tcactttcca tttaaccaat gcgcaaatga aactcaatac aacaaaccgc tgccaaagta
      781 tttctaaagc aattttaaca ggagcaattg attgcccata ctttaaaaat taataacact
      841 gatagtgcta gtgtagatca ctactagagc caggcatcaa ataaaacgaa aggctcagtc
      901 gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca
      961 cactggctca ccttcgggtg ggcctttctg cgtttatata ctagagacct gtaggatcgt
     1021 acaggtttac gcaagaaaat ggtttgttat agtcgaataa atactagaga aagaggagaa
     1081 atactagatg gcgctgaaag ttctgtcagt cgcgacccaa atggcgatcc cttctaacct
     1141 gactacttgc cttcagccga gccacttcaa gtcttcgccg aaactgcttt ccagcaccaa
     1201 ctcctctagc cgtagccgtc tgcgtgtgta ctgttctagc agccagctga ccaccgaacg
     1261 tcgtagcggt aactacaacc caagccgttg ggatgttaac tttatccaat ccctgctgtc
     1321 cgactataag gaagacaaac atgtgattcg tgccagtgaa ctggtgaccc tggttaaaat
     1381 ggagctggaa aaagaaaccg atcagatccg tcaactggaa ctgattgatg acctgcaacg
     1441 tatgggctta tcggaccact tccagaatga atttaaagaa atcctgtctt cgatctattt
     1501 ggaccatcat tattacaaaa acccgttccc gaaagaggaa cgtgatctgt attctacgtc
     1561 cctggctttc cgcctgctcc gcgagcacgg ctttcaggtg gctcaagaag ttttcgactc
     1621 cttcaagaac gaagaagggg agttcaaaga atcgctgagc gatgacaccc gcggcttact
     1681 ccagctgtat gaagcgagct tcctgctgac cgaaggcgaa actaccctgg aaagcgcacg
     1741 cgagtttgcc accaaatttc tcgaagaaaa agtaaatgaa ggcggtgtcg atggagatct
     1801 gctgacccgc attgcgtatt ctctggacat cccgctgcac tggcgtatca aacgtccaaa
     1861 cgcgccggta tggattgagt ggtatcgtaa acgtccggac atgaacccag tcgttctgga
     1921 gctggcaatc ctcgatctga acatcgttca ggcgcagttc caggaagaac tgaaagaatc
     1981 tttccgttgg tggcgcaata ccggtttcgt tgaaaaactg ccattcgctc gcgaccgtct
     2041 ggtggagtgc tacttctgga acactggaat tattgaaccg cgccagcatg cttcagcccg
     2101 cattatgatg ggtaaagtta acgctctgat taccgtaatc gatgacattt acgacgtata
     2161 cggcaccctg gaagaactgg aacagtttac cgatctgatc cgtcgttggg atatcaactc
     2221 gatcgatcag ctgccggact acatgcagct gtgcttcctg gctctcaaca acttcgttga
     2281 tgacacgtct tatgacgtta tgaaggaaaa aggtgtaaac gttatcccgt acctgcgcca
     2341 gagctgggtt gatctggcgg acaagtatat ggtcgaagca cgttggttct acggtggcca
     2401 taaaccgtct ctggaagagt atctggagaa ctcttggcag agcatcagcg gcccgtgcat
     2461 gctcacccac atcttcttcc gtgttaccga ttctttcacc aaagaaactg tggattcgct
     2521 gtataaatac cacgatctgg tccgttggtc ttctttcgtg ctgcgcctgg ctgacgacct
     2581 gggcaccagc gtagaagaag tcagccgtgg tgatgtaccg aagtcgctgc aatgttacat
     2641 gtctgattac aacgcatctg aagcggaagc tcgcaaacat gtgaaatggt tgatcgctga
     2701 ggtttggaaa aagatgaacg ccgaacgtgt gtccaaagat agcccattcg gtaaggactt
     2761 tattggttgc gctgtggacc tgggtcgtat ggcacagctg atgtaccaca acggtgatgg
     2821 tcacggcacc cagcacccga tcatccacca acagatgacg cgcaccctgt ttgaaccgtt
     2881 cgcttaatac tagagccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct
     2941 ttcgttttat ctgttgtttg tcggtgaacg ctctctacta gagtcacact ggctcacctt
     3001 cgggtgggcc tttctgcgtt tata
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