LOCUS       BBa_P2002                443 bp    DNA     linear       10-May-2024
DEFINITION  PoPS->Zif23-GCN4
ACCESSION   BBa_P2002
VERSION     BBa_P2002.1  
FEATURES             Location/Qualifiers
     RBS             1..15
                     /label=RBS-1\Strong
     RBS             8..12
                     /label=RBS
     misc_feature    1..15
                     /label=BBa_B0030
     RBS             1..15
                     /label=BBa_B0030
     misc_feature    22..24
                     /label=start codon
     misc_feature    25..111
                     /label=Zif268 finger 2
     misc_feature    64..84
                     /label=DNA recognition site
     misc_feature    112..171
                     /label=Zif268 finger 3
     misc_feature    148..168
                     /label=DNA recognition site
     misc_feature    172..186
                     /label=domain linker
     misc_feature    187..282
                     /label=GCN4 homodimerization domain
     misc_feature    283..300
                     /label=hexaHis tag
     misc_feature    301..306
                     /label=double stop codon
     CDS             22..306
                     /label=BBa_C2002
     stem_loop       326..369
                     /label=stem_loop
     misc_feature    315..394
                     /label=BBa_B0010
     terminator      315..394
                     /label=BBa_B0010
     stem_loop       410..429
                     /label=T7 TE
     misc_feature    436..436
                     /label=stop
     polyA_site      430..443
                     /label=polya
     misc_feature    403..443
                     /label=BBa_B0012
     terminator      403..443
                     /label=BBa_B0012
ORIGIN
        1 attaaagagg agaaatacta gatgaaaccg ttccagtgcc gtatctgcat gcgtaacttc
       61 tcccgttccg accacctgac cacccacatc cgtacccaca ccggtgaaaa accgttcgct
      121 tgcgacatct gcggtcgtaa attcgctcgt tccgacgaac gtaaacgtca ccgtaaattg
      181 cagcacatga aacagctgga agacaaagtt gaagaactgc tgtccaaaaa ctaccacctg
      241 gaaaacgaag ttgctcgtct gaaaaaactg gttggtgaac gtcatcacca tcaccatcac
      301 taataatact agagccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt
      361 tcgttttatc tgttgtttgt cggtgaacgc tctctactag agtcacactg gctcaccttc
      421 gggtgggcct ttctgcgttt ata
//