LOCUS       BBa_P20020               505 bp    DNA     linear       28-May-2024
DEFINITION  Constitutive expression of Zif23-GCN4
ACCESSION   BBa_P20020
VERSION     BBa_P20020.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     RBS             63..77
                     /label=RBS-1\Strong
     RBS             70..74
                     /label=RBS
     misc_feature    63..77
                     /label=BBa_B0030
     RBS             63..77
                     /label=BBa_B0030
     misc_feature    84..86
                     /label=start codon
     misc_feature    87..173
                     /label=Zif268 finger 2
     misc_feature    126..146
                     /label=DNA recognition site
     misc_feature    174..233
                     /label=Zif268 finger 3
     misc_feature    210..230
                     /label=DNA recognition site
     misc_feature    234..248
                     /label=domain linker
     misc_feature    249..344
                     /label=GCN4 homodimerization domain
     misc_feature    345..362
                     /label=hexaHis tag
     misc_feature    363..368
                     /label=double stop codon
     CDS             84..368
                     /label=BBa_C2002
     stem_loop       388..431
                     /label=stem_loop
     misc_feature    377..456
                     /label=BBa_B0010
     terminator      377..456
                     /label=BBa_B0010
     stem_loop       472..491
                     /label=T7 TE
     misc_feature    498..498
                     /label=stop
     polyA_site      492..505
                     /label=polya
     misc_feature    465..505
                     /label=BBa_B0012
     terminator      465..505
                     /label=BBa_B0012
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agattaaaga ggagaaatac tagatgaaac cgttccagtg ccgtatctgc atgcgtaact
      121 tctcccgttc cgaccacctg accacccaca tccgtaccca caccggtgaa aaaccgttcg
      181 cttgcgacat ctgcggtcgt aaattcgctc gttccgacga acgtaaacgt caccgtaaat
      241 tgcagcacat gaaacagctg gaagacaaag ttgaagaact gctgtccaaa aactaccacc
      301 tggaaaacga agttgctcgt ctgaaaaaac tggttggtga acgtcatcac catcaccatc
      361 actaataata ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc
      421 tttcgtttta tctgttgttt gtcggtgaac gctctctact agagtcacac tggctcacct
      481 tcgggtgggc ctttctgcgt ttata
//