LOCUS       BBa_Q09400               912 bp    DNA     linear       29-May-2024
DEFINITION  tetR inverter, RBS2
ACCESSION   BBa_Q09400
VERSION     BBa_Q09400.1  
FEATURES             Location/Qualifiers
     RBS             1..22
                     /label=BBa_B0039
     CDS             32..648
                     /label=tetR
     misc_feature    649..682
                     /label=SsrA
     misc_feature    689..713
                     /label=Help:Barcodes
     CDS             29..688
                     /label=BBa_C0040
     stem_loop       733..776
                     /label=stem_loop
     misc_feature    722..801
                     /label=BBa_B0010
     terminator      722..801
                     /label=BBa_B0010
     stem_loop       817..836
                     /label=T7 TE
     misc_feature    843..843
                     /label=stop
     polyA_site      837..850
                     /label=polya
     misc_feature    810..850
                     /label=BBa_B0012
     terminator      810..850
                     /label=BBa_B0012
     misc_feature    859..877
                     /label=TetR 1
     misc_feature    859..912
                     /label=BBa_R0040
     promoter        878..883
                     /label=-35
     misc_feature    884..902
                     /label=TetR 2
     promoter        901..906
                     /label=-10
     promoter        859..912
                     /label=BBa_R0040
ORIGIN
        1 tcacacagga aaggcctcga tgtactagat gtccagatta gataaaagta aagtgattaa
       61 cagcgcatta gagctgctta atgaggtcgg aatcgaaggt ttaacaaccc gtaaactcgc
      121 ccagaagcta ggtgtagagc agcctacatt gtattggcat gtaaaaaata agcgggcttt
      181 gctcgacgcc ttagccattg agatgttaga taggcaccat actcactttt gccctttaga
      241 aggggaaagc tggcaagatt ttttacgtaa taacgctaaa agttttagat gtgctttact
      301 aagtcatcgc gatggagcaa aagtacattt aggtacacgg cctacagaaa aacagtatga
      361 aactctcgaa aatcaattag cctttttatg ccaacaaggt ttttcactag agaatgcatt
      421 atatgcactc agcgctgtgg ggcattttac tttaggttgc gtattggaag atcaagagca
      481 tcaagtcgct aaagaagaaa gggaaacacc tactactgat agtatgccgc cattattacg
      541 acaagctatc gaattatttg atcaccaagg tgcagagcca gccttcttat tcggccttga
      601 attgatcata tgcggattag aaaaacaact taaatgtgaa agtgggtccg ctgcaaacga
      661 cgaaaactac gctttagtag cttaataaca ctgatagtgc tagtgtagat cactactaga
      721 gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt
      781 tgtttgtcgg tgaacgctct ctactagagt cacactggct caccttcggg tgggcctttc
      841 tgcgtttata tactagagtc cctatcagtg atagagattg acatccctat cagtgataga
      901 gatactgagc ac
//