BBa_B0030
1
BBa_B0030
RBS.1 (strong) -- modified from R. Weiss
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
Strong RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>.
false
true
_44_46_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("orig" in figure 4-14 of Ron Weiss thesis). <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation1701
1
RBS-1\Strong
range1701
1
1
15
annotation7025
1
BBa_B0030
range7025
1
1
15
annotation1702
1
RBS
range1702
1
8
12
BBa_B0010
1
BBa_B0010
T1 from E. coli rrnB
2003-11-19T12:00:00Z
2015-08-31T04:07:20Z
Transcriptional terminator consisting of a 64 bp stem-loop.
false
false
_1_
0
24
7
In stock
false
true
Randy Rettberg
annotation4184
1
stem_loop
range4184
1
12
55
annotation7018
1
BBa_B0010
range7018
1
1
80
BBa_C2005
1
BBa_C2005
Homodimeric zinc finger
2006-10-20T11:00:00Z
2015-08-31T04:07:25Z
This part is derived from BBa_C2002.
This is protein based on Scot Wolfe's Zif268-GCN4 homodimeric zinc finger protein. It is a fusion of fingers 2 and 3 of Zif268 (mouse) and the leucine zipper domain of GCN4 (yeast).
false
false
_41_
0
126
70
Not in stock
false
This protein contains no tags (like an LVA tag or His tag).
false
Reshma Shetty
annotation1903906
1
GCN4 leucine zipper homodimerization domain
range1903906
1
166
261
annotation1903902
1
finger 2 of Zif268
range1903902
1
4
90
annotation1903904
1
start codon
range1903904
1
1
3
annotation1903905
1
domain linker
range1903905
1
151
165
annotation1903907
1
double stop codon
range1903907
1
262
267
annotation1903903
1
finger 3 of Zif268
range1903903
1
91
150
annotation1903901
1
Zif268.GCN4
range1903901
1
1
267
BBa_Q200514
1
BBa_Q200514
Dimeric zinc finger based inverter
2007-03-27T11:00:00Z
2015-05-08T01:14:14Z
To be entered.
To be entered.
false
false
_41_
0
126
70
It's complicated
false
To be entered.
false
Reshma Shetty
component1922889
1
BBa_R2114
component1922879
1
BBa_B0010
component1922869
1
BBa_B0030
component1922878
1
BBa_C2005
component1922881
1
BBa_B0012
annotation1922878
1
BBa_C2005
range1922878
1
22
288
annotation1922879
1
BBa_B0010
range1922879
1
297
376
annotation1922889
1
BBa_R2114
range1922889
1
434
505
annotation1922881
1
BBa_B0012
range1922881
1
385
425
annotation1922869
1
BBa_B0030
range1922869
1
1
15
BBa_B0012
1
BBa_B0012
TE from coliphageT7
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
Released HQ 2013
Transcription terminator for the <i>E.coli</i> RNA polymerase.
false
false
_1_
0
24
7
In stock
false
<P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
false
Reshma Shetty
annotation1687
1
stop
range1687
1
34
34
annotation1690
1
polya
range1690
1
28
41
annotation1686
1
T7 TE
range1686
1
8
27
annotation7020
1
BBa_B0012
range7020
1
1
41
BBa_R2114
1
BBa_R2114
Promoter with operator site for C2003
2006-10-22T11:00:00Z
2015-05-08T01:14:16Z
This part was inadvertently made while trying to make BBa_R2111. It contains a single base pair mutation in the -10 site of BBa_R2111.
This promoter is designed to bind to C2003.
false
false
_41_
0
126
70
It's complicated
false
The placement of the operator site was designed to be near optimal for steric interference between C2003 and RNA polymerase.
false
Reshma Shetty
annotation1904662
1
C2003 operator site
range1904662
1
23
34
annotation1904659
1
-10 site
range1904659
1
40
45
annotation1904660
1
-35 site
range1904660
1
17
22
annotation1904661
1
transcription start site
range1904661
1
53
53
BBa_B0010_sequence
1
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
BBa_R2114_sequence
1
tttatcaaaaagagtgttgactcccacgcgtgggaataggatatttagattcataaatttgagagaggagtt
BBa_B0030_sequence
1
attaaagaggagaaa
BBa_C2005_sequence
1
atgaaaccgttccagtgccgtatctgcatgcgtaacttctcccgttccgaccacctgaccacccacatccgtacccacaccggtgaaaaaccgttcgcttgcgacatctgcggtcgtaaattcgctcgttccgacgaacgtaaacgtcaccgtaaattgcagcacatgaaacagctggaagacaaagttgaagaactgctgtccaaaaactaccacctggaaaacgaagttgctcgtctgaaaaaactggttggtgaacgttaataa
BBa_B0012_sequence
1
tcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_Q200514_sequence
1
attaaagaggagaaatactagatgaaaccgttccagtgccgtatctgcatgcgtaacttctcccgttccgaccacctgaccacccacatccgtacccacaccggtgaaaaaccgttcgcttgcgacatctgcggtcgtaaattcgctcgttccgacgaacgtaaacgtcaccgtaaattgcagcacatgaaacagctggaagacaaagttgaagaactgctgtccaaaaactaccacctggaaaacgaagttgctcgtctgaaaaaactggttggtgaacgttaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtttatcaaaaagagtgttgactcccacgcgtgggaataggatatttagattcataaatttgagagaggagtt
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z