lux pL

BBa_R0063 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_R0063
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr
Date created: 2003-01-31 12:00:00
Date modified: 2015-05-08 01:14:15

Promoter (luxR & HSL regulated -- lux pL)



    • Details

    Types
    DnaRegion

    Roles
    Regulatory

    promoter

    Sequences BBa_R0063_sequence (Version 1)

    Description

    The lux cassette of V. fischeri contains a left and a right promoter. The left promoter gives weak constitutive expression of downstream genes.This expression is down-regulated by the action of the Lux repressor, LuxR. Two molecules of LuxR protein form a complex with two molecules the signalling compound homoserine lactone (HSL). This complex binds to a palindromic site on the promoter, increasing the rate of transcription from Pr, repressing transcription from Pl



    Notes

    This promoter is based on the Vibrio fischeri quorum sensing gene promoters. Two genes LuxI and LuxR and transcribed in opposite directions as shown below. The original sequence from which the parts BBa_R0062 and BBa_R0063 were derived is shown in the picture below.Includes most of Lux reulatory region, including the LuxR binding site which activates the right promoter. A putative LuxR autorepression binding site is also identified adjacent to the -10 site of the right promoter. This 2nd site has 55% identity with the first site. Putative inverted repeats (of size 18-27 bp) also exist between these two sites (not marked above), which may represent binding sites for other regulatory proteins.

    Unspecified.

    Source

    V. fischeri.

    Sequence Annotation Location Component / Role(s)
    LuxR/HSL
    -10
    -35
    start
    Putative LuxR/HSL
    BBa_R0063
    		1,20
    115,122
    89,94
    128,128
    130,149
    1,151
    		non_covalent_binding_site feature/binding
    feature/promoter promoter
    feature/promoter promoter
    feature/start start_codon
    non_covalent_binding_site feature/binding
    feature/BioBrick engineered_region

    igem#experience
    None
     
    igem#partStatus
    Released HQ 2013
    igem#sampleStatus
    In stock
    igem#status
    Available
     
    synbiohub#ownedBy
    user/james
     
    synbiohub#ownedBy
    user/myers
     
    synbiohub#topLevel
    BBa_R0063/1
     

    Attachments

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