RhlR+C4

BBa_R0071 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_R0071
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Ronny Krashinsky
Date created: 2004-01-24 12:00:00
Date modified: 2015-05-08 01:14:15

Promoter (RhlR & C4-HSL regulated)



    • Details

    Types
    DnaRegion

    Roles
    promoter

    Regulatory

    Sequences BBa_R0071_sequence (Version 1)

    Description

    Promoter activated by RhlR in concert with C4-HSL. C4-HSL is produced by RhlI, and acts as a quorum-sensing autoinducer.


    This is the natural sequence taken from Pseudomonas aeruginosa.


    Crosstalk: this promoter is also activated at a low level by LasR with its associated HSL.

    Notes

    The -10 and -35 sites are labeled as identified in [Pearson97].


    The part begins with the RhlR binding site, and (rather arbitrarily) ends with the first transcribed base (+1).

    Source

    Pseudomonas aeruginosa rhlAB promoter

    Sequence Annotation Location Component / Role(s)
    RhlR
    -35
    -10
    start
    		1,20
    19,24
    42,47
    53,53
    		non_covalent_binding_site feature/binding
    feature/promoter promoter
    promoter feature/promoter
    start_codon feature/start

    igem#experience
    None
     
    igem#partStatus
    Released HQ 2013
    igem#sampleStatus
    In stock
    igem#status
    Available
     
    synbiohub#ownedBy
    user/james
     
    synbiohub#ownedBy
    user/myers
     
    synbiohub#topLevel
    BBa_R0071/1
     

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