Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: crackdots
Date created: 2004-01-27 12:00:00
Date modified: 2015-05-08 01:14:15
Promoter (cinR and HSL regulated, RBS+)
| Types | DnaRegion |
| Roles | promoter Regulatory |
| Sequences | BBa_R0077_sequence (Version 1) |
Description
CinR (BBa_C0077) in complex with O3-C14:1-HSL (from CinI, BBa_C0076) binds to this promoter and activates transcription.This promoter contains the RBS: TGGAGG
Notes
The regulatory locus cinRI in Rhizobium leguminosarum conrols a network of quorum-sensing lociLithgow, JK; Wilkinson, A; Hardman, A; Rodelas, B; Wisniewski-Dye, F; Williams, P; Downie, AJ
MOL. MICROBIOLOGY 37(1): 81-97, 2000
Change log: the last 5 bases (gctaa) removed to allow for the correct spacing between the RBS (TGGAGG) and the start of the downstream gene after biobricks splicing.
Source
Rhizobium leguminosarum| Sequence Annotation | Location | Component / Role(s) |
| embedded RBS stem_loop stem_loop unidentified/uncharacterized CinR dependent | 226,231 35,74 91,136 1,220 | feature/rbs ribosome_entry_site stem_loop feature/stem_loop feature/stem_loop stem_loop promoter feature/promoter |