BBa_R0083
1
OmpR
Promoter (OmpR, positive)
2004-01-27T12:00:00Z
2015-05-08T01:14:15Z
NC_000193 E. coli K12
Released HQ 2013
Positively regulated, OmpR-controlled promoter. This promoter is derived from the upstream region of ompC. Phosphorylated OmpR binds to the operator site and activates transcription. This is a truncated version of BBa_R0082.
false
false
_1_
0
24
7
In stock
false
The promoter deletes the C2 and C3 regions of BBa_R0082 (bases 37-74 in BBa_R0082) and inserts an 8-base linker before the -35 and -10 signal region. The efficacy of this promoter versus the full ompC upstream region (BBa_R0082) or the ompF upstream region (BBa_R0084) is currently unknown.
The a at base 41 is mutated to t to avoid XbaI
site. Speculation that this was a previous assembly site.
true
Stephen Lee, Roshan Kumar, Joe Levine, Ziyan Chu (Polkadorks, IAP 2004)
annotation301223
1
linker
range301223
1
37
44
annotation306881
1
A
range306881
1
41
41
annotation301226
1
-10
range301226
1
68
73
annotation301224
1
-35
range301224
1
45
50
annotation301221
1
C1 OmpR
range301221
1
13
30
BBa_R0083_sequence
1
tcccttgcatttacattttgaaacatctatagcgatctcttgagttggattattctgcatttttggggagaatggact
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z