BBa_R0186

BBa_R0186 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_R0186
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Bartholomew Canton
Date created: 2008-04-07 11:00:00
Date modified: 2015-05-08 01:14:15

T7 promoter (lacI repressible)



Types
DnaRegion

Roles
promoter

Regulatory

Sequences BBa_R0186_sequence (Version 1)

Description

A lac repressible T7 promoter. This promoter is similar to those found in the pET vectors with T7lac promoters. I mutated the -9 base to an A, which should make it less than 3% of the strength of consensus. The base numbering is as used by Studier and coworkers (JMB 1991, 219, 45-59). See BBa_R0183 for more information.

Notes

The -9 mutation from consensus was included to weaken the consensus promoter as described by Imburgio and co-workers (see BBa_R0183 for more information)

Source

This sequence is based on the T7lac promoter described by Dubendorff and Studier (JMB 1991, 219, 45-59). The only modification is the mutation at -9.

Sequence Annotation Location Component / Role(s)
LacI binding site
T7 promoter
20,44
1,20
feature/binding non_covalent_binding_site
promoter feature/promoter
igem#experience
None
 
igem#sampleStatus
Not in stock
igem#status
Unavailable
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_R0186/1