Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Reshma Shetty, Drew Endy
Date created: 2003-01-31 12:00:00
Date modified: 2015-05-08 01:14:15
Promoter, Standard (HK022 cI regulated)
| Types | DnaRegion |
| Roles | Regulatory promoter |
| Sequences | BBa_R1050_sequence (Version 1) |
Description
[Note: This is the same part as R0050 except that the -10 and -35 sites and spacing have been changed to comply with BBa_S0001].The pR regulatory region is a 98 base-pair sequence with standard BioBrick prefix and suffix sections on its ends. It contains two operator sites at which HK022 cI protein can bind cooperatively. The -35 and -10 regions from pR are included along with some flanking sequences. The cutoff for the 5' end of the regulatory region was placed just after the pM -10 region which promotes transcription of a gene upstream (thus the pM -10 region is not included). The cutoff for the 3' end of the regulatory region was placed immediately prior to the RBS.
Notes
Derived from
Modified to comply with BBa_S0001:
TTGACA-17N-GATACT
Literature claims that there is no cross-talk between HK022 cI regulatory region, Lambda cI, 434 cI and P22 cI, but this has not been verified experimentally by the synthetic biology group at MIT.
Source
Bacteriophage HK022.| Sequence Annotation | Location | Component / Role(s) |
| -35 OR2 OR1 putative -10 BBa_R1050 | 8,14 14,28 38,52 44,44 31,36 1,56 | promoter feature/promoter non_covalent_binding_site feature/binding feature/binding non_covalent_binding_site start_codon feature/start promoter feature/promoter engineered_region feature/BioBrick |