cI lam

BBa_R1051 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_R1051
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Vinay S Mahajan, Brian Chow, Peter Carr, Voichita Marinescu and Alexander D. Wissner-Gross
Drew Endy
Date created: 2003-01-31 12:00:00
Date modified: 2015-05-08 01:14:15

Promoter, Standard (lambda cI regulated)



    • Details

    Types
    DnaRegion

    Roles
    promoter

    Regulatory

    Sequences BBa_R1051_sequence (Version 1)

    Description

    [Note: This is the same part as R0051 except that the -10 and -35 sites and spacing have been changed to comply with BBa_S0001].

    The cI regulated promoter is based on the pR promtoer from bacteriohage lambda. The promoter has two two DNA binding sites for lambda cI repressor BBa_C0051. cI binding results in repression of transcription. The specific sequence used here is based on the cI repressible promoter used in the Elowitz repressilator (and references therein).



    Notes

    In order to address concerns about the promoter transcribing in the reverse direction, we have removed the -35 and -10 signals responsible for the promoter activity in the reverse direction. (More details needed here! DE, 2/24/03)

    Modified to comply with BBa_S0001:
    TTGACA-17N-GATACT

    Incompatible with host expressing cI repressor.

    Source

    A synthetic oscillatory network of transcriptional regulators , Elowitz M.B. , Leibler S., Nature(403),335-38: 2000

    Sequence Annotation Location Component / Role(s)
    -35
    OR1
    OR2
    -10
    BBa_R1051
    		15,20
    25,41
    1,17
    38,43
    1,49
    		feature/promoter promoter
    promoter feature/promoter
    promoter feature/promoter
    promoter feature/promoter
    engineered_region feature/BioBrick

    igem#experience
    None
     
    igem#partStatus
    Released HQ 2013
    igem#sampleStatus
    In stock
    igem#status
    Available
     
    synbiohub#ownedBy
    user/james
     
    synbiohub#ownedBy
    user/myers
     
    synbiohub#topLevel
    BBa_R1051/1
     

    Attachments

    © 2018 Newcastle University, University of Utah, and collaborators
    About SynBioHub | View Source on Github | Report an Issue
    | v1.6.1 (fc7e8fac)