Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr
Drew Endy
Date created: 2003-01-31 12:00:00
Date modified: 2015-05-08 01:14:15
Promoter, Standard (luxR and HSL regulated -- lux pR)
| Types | DnaRegion |
| Roles | promoter Regulatory |
| Sequences | BBa_R1062_sequence (Version 1) |
Description
[Note: This is the same part as R0062 except that the -10 and -35 sites and spacing have been changed to comply with BBa_S0001].Promoter activated by LuxR in concert with HSL
The lux cassette of V. fischeri contains a left and a right promoter. The right promoter gives weak constitutive expression of downstream genes.This expression is up-regulated by the action of the LuxR activator protein complexed with the autoinducer, 3-oxo-hexanoyl-HSL. Two molecules of LuxR protein form a complex with two molecules of the signalling compound homoserine lactone (HSL). This complex binds to a palindromic site on the promoter, increasing the rate of transcription.
Notes
This promoter is based on the Vibrio fischeri quorum sensing gene promoters. Two genes LuxI and LuxR and transcribed in opposite directions as shown below. The original sequence from which the parts
Modified to comply with BBa_S0001:
TTGACA-17N-GATACT