BBa_B0100
1
OmpA 5
OmpA 5
2007-02-28T12:00:00Z
2015-08-31T04:07:21Z
MG1655 genome, OmpA gene
5 prime UTR from the OmpA gene in E.coli used for stabilizing the downstream RNA transcript
false
false
_11_
0
571
10
Not in stock
false
This region was PCR amplified from the genome of MG1655.
false
Heather Keller
annotation1919681
1
hp1
range1919681
1
1
63
annotation1919682
1
ss1
range1919682
1
64
74
annotation1919684
1
ss2
range1919684
1
104
115
annotation1919683
1
hp2
range1919683
1
75
103
BBa_B0104
1
AvrII/XbaI
AvrII/XbaI mixed site
2007-03-06T12:00:00Z
2015-08-31T04:07:21Z
n/a
This is the 6 bp mixed site that results when ligating the compatible ends (CTAG 5' overhang) that result from AvrII cleavage (CCTAGA) of the upstream part and XbaI (TCTAGA) cleavage of the downstream part.
false
false
_11_
0
571
10
Not in stock
false
n/a
false
Heather Keller
annotation1920669
1
AvrII/XbaI mixed site
range1920669
1
1
6
BBa_S00164
1
BBa_S00164
R0011 + B0100 + AvrII/XbaI scar
2007-03-06T12:00:00Z
2015-05-08T01:14:16Z
n/a
This is a theoretical intermediate part that will never be physically built, but is necessary for creating the part files of my RBS measurement devices which do not use Biobricks Cloning Sites for inserting the RBS sequence. This is the region found upstream of the RBS sequence in all of the GFP and mCherry measurement devices, including the AvrII/XbaI scar site that is created between this part and the RBS during cloning.
false
false
_11_
0
571
10
Not in stock
false
n/a
false
Heather Keller
component1920702
1
BBa_R0011
component1920711
1
BBa_B0100
component1920713
1
BBa_B0104
annotation1920711
1
BBa_B0100
range1920711
1
56
170
annotation1920702
1
BBa_R0011
range1920702
1
1
54
annotation1920713
1
BBa_B0104
range1920713
1
171
176
BBa_R0011
1
lacI+pL
Promoter (lacI regulated, lambda pL hybrid)
2003-01-31T12:00:00Z
2015-05-08T01:14:14Z
represillator of Elowitz and Leibler (2000)
Released HQ 2013
Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) <p> The PLlac 0-1 promoter is a hybrid regulatory region consisting of the promoter P(L) of phage lambda with the cI binding sites replaced with lacO1. The hybrid design allows for strong promotion that can nevertheless be tightly repressed by LacI, the Lac inhibitor (i.e. repressor) (<bb_part>BBa_C0010</bb_part>) ([LUTZ97]). The activity of the promoter can be regulated over a >600-fold range by IPTG in E.Coli DH5-alpha-Z1 (same paper reference).
false
true
_1_
0
24
7
In stock
false
<P> <P>hybrid promoter design to create strong promoter that is, at the same time, highly repressible. note that the upstream operator installed in this hybrid is slightly different than the one in the original source (Lutz and Bujard, 1997). the most upstream operator region is slightly truncated in the represillator version, so that both operators in the hybrid are the same sequence. see references for details. also, the sequence has been truncated after the transcriptional start site.<P>LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and increase transcription. This part is incompatible with environments containing lactose or lactose analogs.
true
Neelaksh Varshney, Grace Kenney, Daniel Shen, Samantha Sutton
annotation2002
1
-10
range2002
1
43
48
annotation2001
1
lac O1
range2001
1
26
42
annotation2000
1
-35
range2000
1
20
25
annotation7064
1
BBa_R0011
range7064
1
1
54
annotation1999
1
lac O1
range1999
1
3
19
BBa_B0100_sequence
1
gccaggggtgctcggcataagccgaagatatcggtagagttaatattgagcagatcccccggtgaaggatttaaccgtgttatctcgttggagatattcatggcgtattttggat
BBa_S00164_sequence
1
aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacagccaggggtgctcggcataagccgaagatatcggtagagttaatattgagcagatcccccggtgaaggatttaaccgtgttatctcgttggagatattcatggcgtattttggatcctaga
BBa_B0104_sequence
1
cctaga
BBa_R0011_sequence
1
aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcaca
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z