LOCUS       BBa_S01005              1903 bp    DNA     linear       30-Apr-2024
DEFINITION  E0432.R0071.E0422
ACCESSION   BBa_S01005
VERSION     BBa_S01005.1  
FEATURES             Location/Qualifiers
     misc_feature    5..8
                     /label=conserved
     RBS             1..12
                     /label=BBa_B0034
     CDS             19..780
                     /label=YFP (LVA)
     misc_feature    775..780
                     /label=2
     misc_feature    736..774
                     /label=SsrA
     misc_feature    260..260
                     /label=A (Q->L)
     misc_feature    19..780
                     /label=BBa_E0032
     misc_feature    19..780
                     /label=BBa_E0032
     stem_loop       800..843
                     /label=stem_loop
     misc_feature    789..868
                     /label=BBa_B0010
     terminator      789..868
                     /label=BBa_B0010
     stem_loop       884..903
                     /label=T7 TE
     misc_feature    910..910
                     /label=stop
     polyA_site      904..917
                     /label=polya
     misc_feature    877..917
                     /label=BBa_B0012
     terminator      877..917
                     /label=BBa_B0012
     misc_feature    926..945
                     /label=RhlR
     promoter        944..949
                     /label=-35
     promoter        967..972
                     /label=-10
     misc_feature    978..978
                     /label=start
     promoter        926..978
                     /label=BBa_R0071
     misc_feature    991..994
                     /label=conserved
     RBS             987..998
                     /label=BBa_B0034
     CDS             1005..1766
                     /label=CFP (LVA)
     misc_feature    1723..1760
                     /label=SsrA
     misc_feature    1761..1766
                     /label=2
     misc_feature    1073..1073
                     /label=A
     misc_feature    1005..1766
                     /label=BBa_E0022
     misc_feature    1005..1766
                     /label=BBa_E0022
     stem_loop       1786..1829
                     /label=stem_loop
     misc_feature    1775..1854
                     /label=BBa_B0010
     terminator      1775..1854
                     /label=BBa_B0010
     stem_loop       1870..1889
                     /label=T7 TE
     misc_feature    1896..1896
                     /label=stop
     polyA_site      1890..1903
                     /label=polya
     misc_feature    1863..1903
                     /label=BBa_B0012
     terminator      1863..1903
                     /label=BBa_B0012
ORIGIN
        1 aaagaggaga aatactagat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc
       61 atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc
      121 gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg
      181 cccgtgccct ggcccaccct cgtgaccacc ttcggctacg gcctgcaatg cttcgcccgc
      241 taccccgacc acatgaagct gcacgacttc ttcaagtccg ccatgcccga aggctacgtc
      301 caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag
      361 ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac
      421 ggcaacatcc tggggcacaa gctggagtac aactacaaca gccacaacgt ctatatcatg
      481 gccgacaagc agaagaacgg catcaaggtg aacttcaaga tccgccacaa catcgaggac
      541 ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg
      601 ctgctgcccg acaaccacta cctgagctac cagtccgccc tgagcaaaga ccccaacgag
      661 aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg
      721 gacgagctgt acaagaggcc tgctgcaaac gacgaaaact acgctttagt agcttaataa
      781 tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt
      841 tatctgttgt ttgtcggtga acgctctcta ctagagtcac actggctcac cttcgggtgg
      901 gcctttctgc gtttatatac tagagtcctg tgaaatctgg cagttaccgt tagctttcga
      961 attggctaaa aagtgttcta ctagagaaag aggagaaata ctagatggtg agcaagggcg
     1021 aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtgaacggcc
     1081 acaagttcag cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga
     1141 agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccctga
     1201 cctggggcgt gcagtgcttc agccgctacc ccgaccacat gaagcagcac gacttcttca
     1261 agtccgccat gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca
     1321 actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc
     1381 tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact
     1441 acatcagcca caacgtctat atcaccgccg acaagcagaa gaacggcatc aaggccaact
     1501 tcaagatccg ccacaacatc gaggacggca gcgtgcagct cgccgaccac taccagcaga
     1561 acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agcacccagt
     1621 ccgccctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga
     1681 ccgccgccgg gatcactctc ggcatggacg agctgtacaa gaggcctgct gcaaacgacg
     1741 aaaactacgc tttagtagct taataatact agagccaggc atcaaataaa acgaaaggct
     1801 cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag
     1861 agtcacactg gctcaccttc gggtgggcct ttctgcgttt ata
//